Rapid Detection of Food Allergens by Microfluidics ELISA-Based Optical Sensor

Weng, Xuan; Gaur, Gautam; Neethirajan, Suresh

doi:10.3390/bios6020024

Cited by 93 publications

(44 citation statements)

References 30 publications

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“…Biosensors detect gliadin contamination in GF food products with promising LOD [43][44][45], but they are not available on the market yet [46].…”

Section: Immunosensors Dipsticks and Lateral Flow Devices Western Blotmentioning

confidence: 99%

Immunological Methods in Gluten Risk Analysis: A Snapshot

Melini¹,

Melini²

2018

Safety

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Gluten is among the 14 major food allergens officially recognized by Regulation (EU) No. 1169/2011. The risk to coeliac patients from gluten presence in the food products they consume is likely due to the unintentional contamination of naturally gluten-free (GF) and GF-labelled products, or to hidden sources of gluten in processed GF products. The aim of this paper is to provide a snapshot of gluten risk analysis, with emphasis on immunological methods currently used in gluten detection. The study highlights that immunoassays have some advantages over other analytical methods in gluten determination and are suitable for routine tests. However, some factors (e.g., complexity of the food matrix, type of the applied antibody, gluten extraction procedures and lack of reference material) affect the reliability of obtained results. Hence, efforts are required at an analytical level to overcome the drawbacks of the immunological methods currently available. Harmonization is necessary, so as to assist both consumers in making safe food choices, and the food industry in gluten risk assessment, management and communication.

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“…Biosensors detect gliadin contamination in GF food products with promising LOD [43][44][45], but they are not available on the market yet [46].…”

Section: Immunosensors Dipsticks and Lateral Flow Devices Western Blotmentioning

confidence: 99%

Immunological Methods in Gluten Risk Analysis: A Snapshot

Melini¹,

Melini²

2018

Safety

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show abstract

“…S a l a h s a t u u j i s e r o l o g i menggunakan metode Enzyme Linked Immunosorbent Assay (ELISA) yang merupakan suatu teknik biokimia dalam bidang imunologi untuk mendeteksi kehadiran antigen berupa protein di dalam suatu sampel (Garcia et al, 1998). Metode ini telah digunakan sebagai alat mempercepat diagnostik seperti di bidang medis (Gourinat et al, 2015), patologi tumbuhan (Sevik & Kose-Tohumcu, 2011) dan juga berbagai bidang industri makanan (Weng, Gaur, & Neethirajan, 2016).…”

Section: Pendahuluanunclassified

Pengembangan Teknik Serologi Untuk Deteksi Dini Penyakit Jamur Akar Putih (Rigidoporus Microporus) Pada Tanaman Karet

Dalimunthe

Tistama

Wahyuni³

et al. 2018

JPK

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Penyakit Jamur Akar Putih (Rigidoporus microporus) merupakan salah satu penyakit penting pada tanaman karet dan menimbulkan kerugian terbesar karena mengakibatkan kematian tanaman dan biaya yang cukup tinggi untuk pengendalian penyakit tersebut. Oleh karena itu, usaha pencegahan (preventive) lebih diutamakan daripada pengobatan (curative). Deteksi dini gejala penyakit JAP secara konvensional masih sulit sehingga serangan patogen sudah sampai pada tahap lanjut (stadia berat). Upaya mempercepat deteksi ini membutuhkan teknologi yang praktis dan mudah diadopsi oleh para pekebun. Perangkat teknologi untuk mendeteksi adanya materi protein dapat menggunakan pemeriksaan serologi. Tujuan dari penelitian ini adalah mengembangkan teknik serologi untuk mendeteksi gejala serangan dini penyakit jamur akar putih. Hasil penelitian menunjukkan bahwa produksi antibodi untuk mendeteksi JAP dapat diperoleh dengan mengimunisasi ayam petelur dengan ekstrak kasar fruiting body atau miselium sebanyak dua kali dengan interval 1 minggu. AbF dan AbM dapat mengenali AgM dan AgF dengan tingkat reaksi yang berbeda. AbM tidak dapat secara spesifik mendeteksi adanya infeksi JAP melalui daun dan kurang sensitif mendeteksi miselium di tanah. Sebaliknya AbF dapat mendeteksi tanaman terserang JAP melalui daun dan dapat mendeteksi miselium di dalam tanah.

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“…Recently, biosensors have become promising devices for analytical methods in several fields, including clinical detection [ 1 , 2 ], the food industry [ 3 , 4 ], and environmental monitoring [ 5 , 6 ], due to their unique high sensitivity, simplicity, lack of sample preparation, and fast analysis time. A biosensor is an analytical device comprised of two components which are biological sensing elements and a transducer.…”

Section: Introductionmentioning

confidence: 99%

Electrospun Chitosan-Gelatin Biopolymer Composite Nanofibers for Horseradish Peroxidase Immobilization in a Hydrogen Peroxide Biosensor

2017

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A biosensor based on chitosan-gelatin composite biopolymers nanofibers is found to be effective for the immobilization of horseradish peroxidase to detect hydrogen peroxide. The biopolymer nanofibers were fabricated by an electrospining technique. Upon optimization of synthesis parameters, biopolymers nanofibers, an average of 80 nm in diameter, were obtained and were then modified on the working electrode surface. The effects of the concentration of enzyme, pH, and concentration of the buffer and the working potential on the current response of the nanofibers-modified electrode toward hydrogen peroxide were optimized to obtain the maximal current response. The results found that horseradish peroxidase immobilization on chitosan-gelatin composite biopolymer nanofibers had advantages of fast response, excellent reproducibility, high stability, and showed a linear response to hydrogen peroxide in the concentration range from 0.1 to 1.7 mM with a detection limit of 0.05 mM and exhibited high sensitivity of 44 µA∙mM−1∙cm−2. The developed system was evaluated for analysis of disinfectant samples and showed good agreement between the results obtained by the titration method without significant differences at the 0.05 significance level. The proposed strategy based on chitosan-gelatin composite biopolymer nanofibers for the immobilization of enzymes can be extended for the development of other enzyme-based biosensors.

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Rapid Detection of Food Allergens by Microfluidics ELISA-Based Optical Sensor

Cited by 93 publications

References 30 publications

Immunological Methods in Gluten Risk Analysis: A Snapshot

Immunological Methods in Gluten Risk Analysis: A Snapshot

Pengembangan Teknik Serologi Untuk Deteksi Dini Penyakit Jamur Akar Putih (Rigidoporus Microporus) Pada Tanaman Karet

Electrospun Chitosan-Gelatin Biopolymer Composite Nanofibers for Horseradish Peroxidase Immobilization in a Hydrogen Peroxide Biosensor

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