Rapid Detection of Extended Spectrum β-Lactamase (ESBL) for Enterobacteriaceae by use of a Multiplex PCR-based Method

Kim, Junyoung; Jeon, Semi; Rhie, Hogeun; Lee, Bok-Kwon; Park, Misun; Lee, Hoanjong; Lee, Jina; Kim, Seonghan

doi:10.3947/ic.2009.41.3.181

Cited by 66 publications

(36 citation statements)

References 10 publications

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“…The animal isolates of E. coli were analyzed through multiplex PCRs for the presence of extended spectrum β-lactamase (ESBL) and virulence factor genes as described in [27,28].…”

Section: Genetic Analysis On E Coli Isolatesmentioning

confidence: 99%

Control of Growth and Persistence of Listeria monocytogenes and β-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings

Cusimano¹,

Stefano²,

Giglia

et al. 2020

Molecules

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The main objective of this study was to evaluate the efficacy of thymol in controlling environmental contamination in food processing facilities. The effect of thymol was tested as an agent to prevent planktonic and bacterial biofilm growth of twenty-five Listeria monocytogenes isolates from a variety of foods and five Escherichia coli isolates from a farm. The E. coli isolates were positive for extended spectrum β-lactamase (ESBL) genes. All isolates and reference strains were susceptible to thymol at Minimum inhibitory concentration (MIC) values ranging from 250 to 800 μg/mL. An interesting activity of interference with biofilm formation of L. monocytogenes and E. coli was found for thymol at sub-MIC concentrations of 200, 100, 75, and 50 μg/mL. Anti-biofilm activity ranging from 59.71% to 66.90% against pre-formed 24-h-old L. monocytogenes biofilms at concentrations of 500 or 800 µg/mL, corresponding to 2× MIC, was determined against free-living forms of six isolates chosen as the best or moderate biofilm producers among the tested strains. The property of thymol to attack L. monocytogenes biofilm formation was also observed at a concentration of 100 µg/mL, corresponding to 1/4 MIC, by using a stainless-steel model to simulate the surfaces in food industries. This study gives information on the use of thymol in food processing setting.

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“…The animal isolates of E. coli were analyzed through multiplex PCRs for the presence of extended spectrum β-lactamase (ESBL) and virulence factor genes as described in [27,28].…”

Section: Genetic Analysis On E Coli Isolatesmentioning

confidence: 99%

Control of Growth and Persistence of Listeria monocytogenes and β-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings

Cusimano¹,

Stefano²,

Giglia

et al. 2020

Molecules

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“…A 12.5 μL PCR master mix reaction buffer, and 0.5 μL TEM F, 0.5 μL TEM R, 1 μL of each remaining primers (10 μM/μL), 2.5 μL H2O, and 5 μL of the template DNA preparation were added to the reaction mixture. Reactions were performed in a DNA thermal cycler (BIOER, Tokyo, Japan) under the following conditions: denaturation at 94°C for 5 minutes followed by 30 cycles at 94°C for 20 seconds, 61°C for 30 seconds, and 72°C for 1 minute with a final extension of 72°C for 5 minutes [23].…”

Section: Characterization Of Genes Encoding Esblsmentioning

confidence: 99%

Multidrug-resistant ESBL-producing Enterobacteriaceae and associated risk factors in community infants in Lebanon

Hijazi

Fawzi

Ali

et al. 2016

J Infect Dev Ctries

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Introduction: Extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) infections are a growing threat to children, and the treatment of these infections becomes more and more challenging. A huge reservoir for ESBLs in the community is the fecal flora of children. This study investigates the rectal colonization, associated risk factors, antimicrobial susceptibility, and molecular characterization of ESBL-PE in Lebanese community infants. Methodology: A total of 117 rectal swabs were taken from healthy infants between 1 and 12 months of age. Detection of ESBLs was carried out using the double-disk synergy test, combination-disk method, and multiplex polymerase chain reaction (PCR). A questionnaire about the infant's history and risk factors for carrying ESBL-PE was administered. Results: In total, 58 (49.6%) of 117 participants were ESBL-PE carriers. Some significant important risk factors for colonization in this study were male gender, hospital birth, caesarean delivery, and being formula-fed. Observed decrease in colonization rate was associated with intimate hygiene habits. Carriers of multiple bla genes were the most common. CTX-M type was the major harbored, gene and CTX-M-9 was the most predominant, followed by CTX-M-15 type. Conclusions: To the best of our knowledge, this is the first available data about the carriage rate of ESBL-PE in community infants in Lebanon and the Middle East, the first study showing that birth in hospital, caesarean delivery, and being formula-fed are all significantly associated risk factors for the high colonization rates in community -not hospitalized -infants, and showing the dominance of multiple resistance gene carriage and wide dissemination of CTX-M-9 ESBL.

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“…All isolates with an ESBL phenotype were tested by multiplex PCR for bla genes of the TEM, SHV, CMY, OXA, DHA, and CTX-M types, and the bla CTX-M genes were characterized by sequencing analysis of the amplicons [14]. For strains with a positive result for bla CTX-M , the physical linkages between an ISEcp1-like element and the bla CTX-M-12 or bla CTX-M-14 genes were determined by PCR and sequencing analysis [1].…”

Section: Genetic Characterization Of Bla Genesmentioning

confidence: 99%

Characterization of CTX-M-Type Extended-Spectrum Beta-Lactamase-Producing Diarrheagenic Escherichia coli Isolates in the Republic of Korea During 2008-2011

Kim¹,

Kim²,

Kim³

et al. 2014

Journal of Microbiology and Biotechnology

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To characterize the extended-spectrum beta-lactamases (ESBLs) in diarrheagenic Escherichia coli from Korea in 2008-2011, we screened seven enterotoxigenic E. coli (ETEC) and one enteroaggregative E. coli (EAEC) that produce ESBLs from a nationwide survey. All eight isolates produced CTX-M-type ESBLs, including CTX-M-12 (n = 4), CTX-M-14 (n = 2), and CTX-M-15 (n = 2). PCR-based replicon typing indicated that the blaCTX-M-12 genes of four ETEC isolates were carried on a conjugative IncF plasmid, whereas the blaCTX-M-14 of one EAEC was located on an IncK plasmid. This is the first report of the occurrence of blaCTX-M genes in clinical isolates of EAEC in Korea. The ESBL-producing isolates were shown to be different based on pulsed-field gel electrophoresis and multilocus sequence typing, whereas the four isolates with CTX-M-12 were clonally related. These observations raise an alarm for the spread of plasmid-mediated resistance to ESBL among diarrheagenic E. coli.

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Rapid Detection of Extended Spectrum β-Lactamase (ESBL) for Enterobacteriaceae by use of a Multiplex PCR-based Method

Cited by 66 publications

References 10 publications

Control of Growth and Persistence of Listeria monocytogenes and β-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings

Control of Growth and Persistence of Listeria monocytogenes and β-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings

Multidrug-resistant ESBL-producing Enterobacteriaceae and associated risk factors in community infants in Lebanon

Characterization of CTX-M-Type Extended-Spectrum Beta-Lactamase-Producing Diarrheagenic Escherichia coli Isolates in the Republic of Korea During 2008-2011

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