Rapid Detection of Eskape Bacteria Using Tapered Dielectrophoresis and Their Presence in Urban Water Cycle

Mohtar, Wan Hanna Melini Wan; Razali, Muhammad Amirul Mohd; Mazlan, Muhammad  Abid; Rozaini, Arash Zulkarnain Ahmad; Hamid, Aidil Abdul; Buyong, Muhamad Ramdzan; Mooralitharan, Silambarasi

doi:10.2139/ssrn.4236280

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“…Dielectrophoresis (DEP) technique is a critical approach on microbial characterization for solving the bacterial infection. Characterization of Staphylococcus aureus using DEP for DEP response pDEP, crossover frequency and nDEP in this recent years is important for detection, manipulation and isolation of the cells [3,4]. Based on past research, DEP devices focusing on 80 µm interelectrode gap for biological application [5][6][7][8][9].…”

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confidence: 99%

Characterization of 20 μm Dielectrophoretic Interelectrode Gap for Staphylococcus Aureus Rapid Detection Application

Arash Zulkarnain Ahmad Rozaini,

Abdullah Abdulhameed,

Revathy Deivasigamani

et al. 2024

IJNeaM

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We propose an improvement of dielectrophoresis technique (DEP) by designing, simulating and experiment a 20 μm interelectrode gap for Staphylococcus aureus rapid detection application. In this paper, we use MyDEP simulation for DEP polarization Staphylococcus aureus on frequencies range. COMSOL simulation is utilized for comparison of 20 μm and 80 μm interelectrode gap based on trajectory and velocity of particles for Staphylococcus aureus application. We implied 20 μm interelectrode gap for Staphylococcus aureus application produced higher magnitude DEP force. Which gives accurate trajectory characterization and higher velocity of particle movement. DEP characterization using small interelectrode gap is capable of producing stable and optimum DEP value. It is demanding to distinguish the simulation Staphylococcus aureus using experimental method. DEP technique is important for analysis and characterization of the bacteria. The result show that 20 μm interelectrode gap has higher intensity of electric field which is 1.51 x 10^6 V/m in COMSOL simulation and produced 20.1 m/s for velocity of particle trajectories which is higher compared to 80 μm interelectrode gap. The DEP response was tested on 2.5 MHz, as crossover frequency response was observed on experimental and simulation. Thus, supportsthe capability of 20 μm interelectrode gap for Staphylococcus aureus rapid detection application. Furthermore, DEP characterization can be improvised by focusing on interelectrode gap for characterization among bacteria cells, critical for bacteria detection, manipulation, and isolation.

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