Rapid detection of Escherichia coli in dairy milk using static headspace-comprehensive two-dimensional gas chromatography

Heng, Wan Sin; Jadhav, Snehal; Ueland, Maiken; Shellie, Robert A.

doi:10.1007/s00216-022-04485-7

Cited by 1 publication

(1 citation statement)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To the best of our knowledge, the effect of the presence of silica on bacterial culture is unknown. The use of broths has previously been shown to increase the percentage of positive cultures on different sample types in many species in comparison with direct bacterial culture (Heng et al., 2022 ; Nocera et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of silica‐sprayed collection tubes on synovial fluid bacterial culture

Jimenez Rihuete,

Martin,

Villarino

et al. 2024

Veterinary Medicine & Sci

View full text Add to dashboard Cite

IntroductionSilica‐sprayed tubes (SSTs) are often used to transport synovial fluid samples in equine practice. They promote the coagulation of the sample. The objective of the study is to evaluate the effect of SST on bacterial culture.Materials and methodsThe study was divided into two parts: sterile saline (Part A) and synovial fluid (Part B). Four common bacteria associated with equine synovial sepsis were used: Streptococcus pyogenes, Escherichia coli, Staphylococcus aureus and methicillin‐resistant S. aureus (MRSA). Three collection tubes were used: STT, plain (no‐additives) and brain and heart infusion (BHI) broth. Bacteria were cultured in horse blood agar plates for 48 h. Outcome variables were negative culture, positive culture and total number of colony‐forming units (CFUs). Statistical analysis was performed using Mann–Whitney U test, and significance was set at p < 0.05.ResultsThe total number of agar plates read was 1557 (779 saline; 778 synovial fluid). Total negative cultures were 25/779 on saline and 3/778 on synovial fluid. In broth, maximum growth CFU was achieved after 8 h for both saline and synovial fluid for all bacteria. S. pyogenesand E. coli produced a significantly lower number of CFU when in SST compared to plain or broth after 4 h, whereas S. aureus (American Type Culture Collection [ATCC] and MRSA) only after 24 h.DiscussionSilica‐containing tubes reduced bacterial proliferation, whereas the use of a BHI broth provided the highest bacterial load in the sample. The use of SST may have a negative effect on bacterial proliferation in samples obtained from clinical cases.

show abstract

Section: Introductionmentioning

confidence: 99%