Rapid Detection of Cytomegalovirus Pneumonia from Lung Lavage Cells

Abstract

Because cytomegalovirus (CMV) is a common cause of fatal pneumonia in the immunocompromised host, a rapid and reliable method to confirm this diagnosis is essential. Bronchoalveolar lavage (BAL) has proved to be a rapid, safe, and sensitive method for the diagnosis of several forms of pneumonia in these patients, but its efficacy for confirming CMV pneumonia remains to be established. In this study, we compared the sensitivity and specificity of conventional viral culture, immunocytochemical staining, and cytological examination performed on cells recovered by BAL for establishing CMV as the cause of pneumonia in 71 BAL specimens from 56 immunocompromised patients. Pneumonia due to CMV was confirmed by stated criteria in 14 of these patients. Virus was isolated by culture in BAL specimens from all patients with CMV pneumonia (sensitivity 100%), but also in 17 specimens from patients who did not have CMV pneumonia (specificity 70%). On cytologic examination, CMV inclusions were found in 3 of the 14 specimens from patients with CMV pneumonia (sensitivity 21%) and also in 1 patient at risk for pneumonia but who did not fulfill the criteria (specificity 98%). Thus, a positive culture and positive cytology virtually confirmed CMV pneumonia, whereas a negative culture excluded it. Immunocytochemistry proved to be particularly useful when the culture was positive and cytology was negative. In this situation, specific labeling of CMV antigen by monoclonal antibody was found in 9 of the 11 patients with CMV pneumonia (sensitivity 82%). Thus, the absence of specific staining in BAL cells tended to exclude CMV as a cause of pneumonia in patients with positive cultures and negative cytologies.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult Onset Still’s Disease Presenting with Acute Respiratory Distress Syndrome: Case Report and Review of the Literature

“…Viral cultures of bronchoscopic alveolar lavage fluid, tested during her first admission were negative for CMV. This culture has a reported sensitivity of 100% and specificity of 70% [2]. Her initial presentation with ARDS is thus unlikely to be attributable to CMV pneumonia, and all other infectious workup was negative.…”

Viral Pneumonia in Patients with Hematologic Malignancy or Hematopoietic Stem Cell Transplantation

“…78 Cytologic examination with demonstration of CMV intracytoplasmic inclusions is highly specific but poorly sensitive. 79 PCR is highly sensitive and specific if the pretest clinical suspicion for CMV pneumonia is high. [80][81][82] In patients without respiratory symptoms, PCR-based results may result in false positives because pulmonary shedding of virus is common in patients with CMV infection without tissue-invasive disease.…”

Cytomegalovirus

“…In a prospective study Paradis et al (1988) studied the comparative sensitivity and specificity of three methods of diagnosing CMV pneumonia by using BAL specimens. These were routine culture, direct immunoperoxidase detection of early antigen, and histological evidence of inclusions.…”