Rapid Detection of Bovine Mycoplasma Antigens by Counterimmunoelectrophoresis

Abstract

Twelve reference strains of mycoplasma and acholeplasma previously reported to have been recovered from cattle were tested against hyperimmune rabbit serum by counterimmunoelectrophoresis. This technique detected antigen by. the formation of precipitin lines with antibody within 1 h and promises to be a useful technique for detecting and identifying mycoplasma isolates in either pure or mixed cultures.

Mycoplasma agalactiae, an Etiological Agent of Contagious Agalactia in Small Ruminants: A Review

“…Serological tests of importance for detecting M. agalactiae include growth precipitation (GP), immunofluorescence (IF), complement fixation test (CFT), indirect haemagglutination (IHA), haemagglutination inhibition (HI), agglutination, latex agglutination test (LAT), double immunodiffusion (DID), single radial immunodiffusion (SRID), enzyme linked immunosorbent assay (ELISA), radio immunoassay (RIA), and immunoperoxidase (IP) [ 1 , 9 , 22 , 87 , 89 – 96 ]. They also include many electrophoretic techniques such as gel electrophoresis, immunoelectrophoresis (IEP), countercurrent immunoelectrophoresis (CCE), and crossed immunoelectrophoresis [ 22 , 40 , 97 , 98 ]. Immunoblotting has been used to demonstrate the antigenic specificity by the use of hyperimmune sera from rabbit which is monospecific [ 42 ].…”

Comparison of antigens of pneumonia-associated mycoplasma species by gel diffusion

“…The significance of these interrelationships in taxonomic as well as biochemical terms is as yet largely unresolved. Common precipitating antigens among various species have been demonstrated by agar gel double immunodiffusion (DID) and counterimmunoelectrophoresis (CIE) techniques (2,3,9,10,12,14), and by means of DID it has been possible to divide a number of species into groups (3,9,10). This paper describes some previously unrecorded interrelationships among the antigens of six mycoplasma species, demonstrated by means of DID and CIE. Five of these have been implicated to varying degrees in pneumonia in different animals; the sixth, Mycoplasma bovoculi, was included for further comparison, having been shown by Erno and Jurmanova (3) to share a common antigen with M. dispar.…”

Rapid detection and quantitation of Clostridium perfringens enterostoxin by counterimmunoelectrophoresis

“…Counterimmunoelectrophoresis (CIEP) is a specific, rapid, sensitive, easy, and accurate means of inducing a precipitin reaction between a particular antigen and its antibody. CIEP has been used in the past for the routine identification of various kinds of antigens (1,5,6,10,11). Here, we describe the use of this technique for rapid detection of C. perfringens enterotoxin.…”