Rapid detection of antimicrobial activity using flow cytometry

Role of Autofluorescence in Flow Cytometric Analysis of Escherichia coli Treated with Bactericidal Antibiotics

“…Still, Durodie et al suggested that cellular protein content increases when cells are treated with antibiotics (47). Moreover, for aquatic and environmental microbes, autofluorescence was assumed to be due to phototrophic pigments and some organic compounds in addition to certain physicochemical properties of the fluorochrome (2).…”

Applications of Flow Cytometry to Clinical Microbiology

“…In addition, FCM is a reliable approach for susceptibility testing, offering results in terms of the bactericidal or bacteriostatic effect (86,108,198,262,331).…”

“…This has been performed with ␤-lactam antibiotics, which affect cell membrane integrity but induce an increase in nucleic acid contents in growing but not dividing cells. DNA ploidy has also been studied by Durodie et al (86) in E. coli with sub-MIC concentrations of ␤-lactam antibiotics. PI, a fluorochrome that intercalates into double-stranded nucleic acid, is a more appropriate stain than ethidium bromide for susceptibility testing by FCM (262).…”

Bacteriostatic Effect of Mixtures of 1% Propofol with 4% Lidocaine versus 4% Lidocaine Alone: Regards on Microbiologic Studies in the Field of Anesthesiology

“…1 Hospitals provide care for the most medically vulnerable and immunocompromised individuals in society; however, despite governmental safety regulations and well-established safe food handling practices, foodborne outbreaks in healthcare settings do occur and are associated with an increased risk of death compared with other settings. 2 Listeria monocytogenes is uniquely suited to cause serious nosocomial infections, given its tendency to contaminate certain ready-to-eat food products, ability to replicate at refrigerator temperatures, and propensity to cause invasive infection in immunocompromised patients and pregnant women. Stem-cell/solid-organ transplant patients have a 2,584-fold greater risk than the general population of developing serious illness from L. monocytogenes infection, while patients with hematologic malignancy, HIV/AIDS, end-stage renal disease, diabetes, alcoholism, and age greater than 65 years have risks of developing serious illness from Listeria that range from 7.5 to 1,384 over that of the general population.…”

“…3 Numerous outbreaks of nosocomial listeriosis have been reported, with hospital-provided sandwiches, ready-to-eat sausage products, diced celery, soft cheeses, and sliced deli meats most frequently implicated as the source of the outbreaks. 2,[4][5][6][7] High mortality rates have been reported in these outbreaks, and most patients who died had some level of compromised immunity.…”