Rapid detection of anticardiolipin antibodies

Abstract

A rapid screening method for the detection of antiphospholipid antibodies is described. Dense, red dyed polystyrene beads coated with cardiolipin were incubated with test sera for a short period of time, then added to a microtube containing anti-human IgG in a gel provided within a pre-cast card (DiaMed ID Microtyping System). The card was centrifuged at 150g for 5 min and then examined for movement of the beads through the gel.

Rapid detection of JMH antibodies with recombinant Sema7A (CD108) protein and the particle gel immunoassay

“…These results show that two types of soluble blood group proteins can be used in the ID‐PaGIA for rapid detection of RBC antibodies. In addition, the fact that a number of different non–blood group antigens have been successfully used in the ID‐PaGIA so far demonstrates that this technology is very flexible and that it can be used for all kinds of antigens available in soluble form 5,8‐10 . As already discussed in detail, 7 the implications for blood group serology are that single‐pass (e.g., Lutheran) and glycosylphosphatidylinositol‐linked proteins (e.g., Sema7A) that can be produced in truncated forms without losing their three‐dimensional structure are good candidates for use in the ID‐PaGIA.…”

Rapid detection of anti‐Lub with recombinant Lub protein and the particle gel immunoassay

“…A number of different antigens have been successfully used in the ID-PaGIA so far. [5][6][7][8] A necessary condition is that the antigens to be coupled to the high-density polystyrene beads must be available in soluble form. For blood group serology, this means that all RBC antigens that can be produced in soluble form could theoretically be used with the ID-PaGIA.…”

Rapid detection of antibodies to immunoglobulin A molecules by using the particle gel immunoassay

“…Red, high‐density polystyrene beads were coated with IgA molecules, as previously described [7,8]. Human polyclonal affinity‐purified IgA, immunoglobulin M (IgM) and immunoglobulin G (IgG), and rabbit polyclonal anti‐IgA, anti‐IgM and anti‐IgG, were obtained from Jackson Laboratories (West Grove, PA).…”

“…Serial dilutions of serum samples (5 µl in NaCl) were incubated with IgA‐coated beads (0·15% solids in particle buffer), using the card containing anti‐human IgG (the so‐called human anti‐IgA test card; DiaMed). After incubation for 5 min at room temperature (18–20 °C), the ID‐Cards were centrifuged for 10 min and the results were read macroscopically, as described previously [7,8]. Positive reactions are demonstrated by agglutination of beads.…”