Rapid Detection of Anti-SARS-CoV-2 IgM and IgG Using a Selenium Nanoparticle-based Lateral Flow Immunoassay

Abstract

Background: Coronavirus disease 2019 is an infectious disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SARS-CoV-2 is highly transmissible. Early and rapid testing is necessary to effectively prevent and control the outbreak. Detection of SARS-CoV-2 antibodies with lateral flow immunoassay can achieve this goal. Antibody detection is especially effective for the detection of asymptomatic infection.Methods: In this study, SARS-CoV-2 nucleoprotein was expressed by E. coli and purified by affinity chromatography. We used the highly stable and sensitive selenium nanoparticle as the labeling probe coupled with the SARS-CoV-2 nucleoprotein to prepare a new SARS-CoV-2 antibody (IgM and IgG) detection kit. The sensitivity and specificity of the kit were verified by plasma of COVID-19 patients and health persons. Separate detection of IgM and IgG, such as in this assay, was performed in order to reduce mutual interference and improve the accuracy of the test results.Results: The SARS-CoV-2 nucleoprotein was purified on a nickel column, and the final purity was greater than 90%. The sensitivity of the kit was 94.74% and the specificity was 95.12% by 41 negative plasma samples and 19 positive plasma samples detection.Conclusions: The assay kit does not require any special device for reading the results and the readout is a simple color change that can be evaluated with the naked eye. This kit is suitable for rapid and real-time detection of the SARS-CoV-2 antibody.

A Minireview on Nanoparticle-Based Sensors for the Detection of Coronaviruses

“…The developed method obtains results within 15 min and needed only 10–20 μl serum for each experiment. Other nanoparticles LFIA kits are selenium nanoparticle-based LFIA for rapid diagonalization of anti-SARS-CoV-2 IgM and IgG in plasma samples [ 17 ] and Au NPs based LFIA for diagonalization of IgG antibody against SARS-CoV-2 virus in serum samples [ 18 ].…”

Hyperoside'nin siklofosfamid kaynaklı apoptoza karşı koruyucu etkilerinin in silico değerlendirilmesi

“…1 Biyokimya Bölümü / Fen edebiyat Fakültesi, Iğdır Üniversitesi, Türkiye 2 Metematik ve Fen Bilgisi Öğretmenliği Bölümü / Eğitim Fakültesi, Siirt Üniversite, Türkiye * bahri.gur@igdir.edu.tr Özet -Hyperoside antiinflamatuar, antibakteriyel, antiviral, nöroprotektif, antidepresan ve organ koruyucu etkilerinin yanısıra son zamanlarda akciğer, servikal, mide, kolon, pankreas, meme ve yumurtalık kanseri üzerine antitümör etkilerinin araştırılmaya başlandığı doğal bir flavonol glikozittir [1]. Çeşitli bitkilerden ekstrakte edildiği gibi kimyasal ve biyosentez yöntemlerle de elde edilmektedir.…”

Recent Advances in Novel Lateral Flow Technologies for Detection of COVID-19

“…In recent years, fluorescent nanoparticles have been increasingly applied in disease diagnosis and are promising alternative reporters, as their unique chemical and optical properties mean that they have the potential to enhance the sensitivity of lateral flow assays. Many fluorescence nanoparticles were utilized for SARS-CoV-2 detection, including QDs [95], FNDs [96], selenium nanoparticles [65,97], up-converting phosphor particles [98], lanthanide-doped nanoparticles [64], and aggregation-induced emission (AIE) nanoparticles [66].…”