Rapid detection of a plant virus by solution hybridization using oligonucleotide probes

Rouhiainen, Leo; Laaksonen, Matti; Karjalainen, Reijo

doi:10.1016/0166-0934(91)90123-h

Cited by 8 publications

(2 citation statements)

References 29 publications

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“…The protein coat found in most plant viruses has allowed the development of direct solid support binding techniques (75,138) that facilitate reliable detection and multiplex systems. Solution hybridization using oligonucleotide probes is a nucleic acid-based ELISA-like approach to diagnosis that facilitates testing of large numbers of samples (135). This system was used to reliably detect potato virus X.…”

Section: Large-scale Testingmentioning

confidence: 99%

Impacts of Molecular Diagnostic Technologies on Plant Disease Management

Martín

James

Lévesque

2000

Annu. Rev. Phytopathol.

216

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Detection and diagnosis of plant viruses has included serological laboratory tests since the 1960s. Relatively little work was done on serological detection of plant pathogenic bacteria and fungi prior to the development of ELISA and monoclonal antibody technologies. Most applications for laboratory-based tests were directed at virus detection with relatively little emphasis on fungal and bacterial pathogens, though there was some good work done with other groups of plant pathogens. With the advent of molecular biology and the ability to compare regions of genomic DNA representing conserved sequences, the development of laboratory tests increased at an amazing rate for all groups of plant pathogens. Comparison of ITS regions of bacteria, fungi, and nematodes has proven useful for taxonomic purposes. Sequencing of conserved genes has been used to develop PCR-based detection with varying levels of specificity for viruses, fungi, and bacteria. Combinations of ELISA and PCR technologies are used to improve sensitivity of detection and to avoid problems with inhibitors or PCR often found in plants. The application of these technologies in plant pathology has greatly improved our ability to detect plant pathogens and is increasing our understanding of, their ecology and epidemiology.

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Section: Large-scale Testingmentioning

confidence: 99%

Impacts of Molecular Diagnostic Technologies on Plant Disease Management

Martín

James

Lévesque

2000

Annu. Rev. Phytopathol.

216

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“…In an attempt to apply non-radioactive detection methods, two different approaches were followed: (i) a sandwich hybridization and affinity-based hybrid collection method (RANKI et al 1983, SWANEN et al 1986, ROUHIAINEN et al 1991, adapted for use with Nylon membranes in which digoxigenin was used as reporter group and (ii) a polymerase chain reaction (PCR) suitable for detection of AMCV sequences in artichoke sap.…”

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confidence: 99%

Use of the Polymerase Chain Reaction and Sandwich‐Hybridization for Detecting Artichoke Mottled Crinkle Tombusvirus in Artichoke

Barbarossa

Grieco

Iosco

et al. 1994

Journal of Phytopathology

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Two non‐radioactive methods were applied for the detection of artichoke mottled crinkle tombusvirus (AMCV) in artichoke plant sap. A sandwich hybridization method was developed using a biotinylated transcript as capture and a digoxigenin‐labelled riboprobe. The hybrid was collected onto Nylon membranes coated with streptavidin using a slot‐blot procedure. The second method was the reverse transcriptional polymerase chain reaction (RT‐PCR) carried out with three different primers obtained from widely, separated sequences of AMCV genome. In artichoke plant sap, RT‐PCR was 20‐fold more sensitive than sandwich‐hybridization and 2.5‐fold less sensitive than dor‐blot hybridization labelled with radioactive reporter groups.

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Expression of PVX coat protein gene under the control of extensin-gene promoter confers virus resistance on transgenic potato plants

et al. 1992

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Tuber discs of potato (Solanum tuberosum L.) cultivars Desirée and Gracia were infected by Agrobacterium tumefaciens carrying a binary vector with the coat protein gene of potato virus X controlled by the carrot extensin gene long-transcript promoter. Several transgenic potato plants have been obtained by direct regeneration of shoots on culture medium with kanamycin used for selection. The presence of the coat protein gene was proved by Southern hybridization in several transformants. Its low but detectable expression level was shown by Northern and Western analysis. Ethephon treatment resulted in a five-fold increase in the amounts of the coat protein mRNA. The majority of transformants exhibited reduced accumulation of virus RNA in inoculated leaves. Potentials in the use of an ethylene-inducible promoter in the production of virus-resistant transgenic plants will be discussed.

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Rapid detection of a plant virus by solution hybridization using oligonucleotide probes

Cited by 8 publications

References 29 publications

Impacts of Molecular Diagnostic Technologies on Plant Disease Management

Impacts of Molecular Diagnostic Technologies on Plant Disease Management

Use of the Polymerase Chain Reaction and Sandwich‐Hybridization for Detecting Artichoke Mottled Crinkle Tombusvirus in Artichoke

Expression of PVX coat protein gene under the control of extensin-gene promoter confers virus resistance on transgenic potato plants

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