Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay

Broughton, James P.; Deng, Xianding; Yu, Guixia; Fasching, Clare L.; Singh, Jasmeet; Streithorst, Jessica; Granados, Andrea; Sotomayor-González, Alicia; Zorn, Kelsey C.; Gopez, Allan; Hsu, Elaine; Gu, Wei; Miller, Steven J.; Pan, Chao-Yang; Guevara, Hugo; Wadford, Debra A.; Chen, Janice; Chiu, Charles Y.

doi:10.1101/2020.03.06.20032334

Cited by 126 publications

(135 citation statements)

References 15 publications

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“…There has also been unprecedented rapid and open sharing of diagnostics and devices through preprint publications, and through informal communication routes among researchers [90,[106][107][108]. For example, agencies in China, Germany, Hong Kong, Japan, Thailand, South Africa and the United States have shared protocols for molecular assays online, including the DNA sequences to detect the virus.…”

Section: Fosh Approaches In Covid-19 Diagnosticsmentioning

confidence: 99%

Leveraging open hardware to alleviate the burden of COVID-19 on global health systems

et al. 2020

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With the current rapid spread of COVID-19, global health systems are increasingly overburdened by the sheer number of people that need diagnosis, isolation and treatment. Shortcomings are evident across the board, from staffing, facilities for rapid and reliable testing to availability of hospital beds and key medical-grade equipment. The scale and breadth of the problem calls for an equally substantive response not only from frontline workers such as medical staff and scientists, but from skilled members of the public who have the time, facilities and knowledge to meaningfully contribute to a consolidated global response. Here, we summarise community-driven approaches based on Free and Open Source scientific and medical Hardware (FOSH) as well as personal protective equipment (PPE) currently being developed and deployed to support the global response for COVID-19 prevention, patient treatment and diagnostics.

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Section: Fosh Approaches In Covid-19 Diagnosticsmentioning

confidence: 99%

Leveraging open hardware to alleviate the burden of COVID-19 on global health systems

et al. 2020

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“…As a result, the prevalence of SARS-CoV-2 in the population is mostly unknown, particularly among mild or asymptomatic cases. Though several novel, scalable biotechnological innovations for viral testing have recently emerged (e.g., CRISPR-Cas12a (Broughton et al, 2020) or CRISPR-Cas13 (Metsky et al, 2020) on paper-based detection systems, or loop-mediated isothermal amplification (LAMP) (Tanner et al, 2015, Schmid-Burgk et. al., 2020 these have not been validated against gold-standard clinical assays or nextgeneration sequencing (NGS).…”

Section: Introductionmentioning

confidence: 99%

Shotgun Transcriptome and Isothermal Profiling of SARS-CoV-2 Infection Reveals Unique Host Responses, Viral Diversification, and Drug Interactions

Butler

Mozsary

Meydan

et al. 2020

Preprint

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The pandemic from the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) led to hundreds of thousands of deaths, including >15,000 in New York City (NYC). This pandemic highlighted a pressing clinical and public health need for rapid, scalable diagnostics that can detect SARS-CoV-2 infection, interrogate strain evolution, and map host response in patients. To address these challenges, we designed a fast (30 minute) colorimetric test to identify SARS-CoV-2 infection and simultaneously developed a large-scale shotgun metatranscriptomic profiling platform for nasopharyngeal swabs. Both technologies were used to profile 338 clinical specimens tested for SARS-CoV-2 and 86 NYC subway samples, creating a broad molecular picture of the COVID-19 epidemic in NYC. Our results nominate a novel, NYC-enriched SARS-CoV-2 subclade, reveal specific host responses in ACE pathways, and find medication risks associated with SARS-CoV-2 infection and ACE inhibitors. Our findings have immediate applications to SARS-CoV-2 diagnostics, public health monitoring, and therapeutic development.

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“…Several attempts to address this challenge have already been suggested, that can be categorized into three major approaches. The first approach is to replace PCR based methods by other direct diagnostic methods such as Loopmediated Isothermal Amplification (LAMP) [1][2][3] and CRISPR based diagnostic tools [4][5][6], the second approach involves serological surveys [7][8][9][10], and the third approach involves the improvement of the PCR methods capacity by optimization and automation (e.g. [11][12][13]) or by reducing the required number of tests via pooling samples together, known as group testing.…”

Section: Introductionmentioning

confidence: 99%

Large-scale implementation of pooled RNA-extraction and RT-PCR for SARS-CoV-2 detection

Ben-Ami

Klochendler

Seidel

et al. 2020

Preprint

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Testing for active SARS-CoV-2 infection is a fundamental tool in public health measures taken to control the COVID-19 pandemic. Due to the overwhelming use of SARS-CoV-2 RT-PCR tests worldwide, availability of test kits has become a major bottleneck. Here we demonstrate the reliability and efficiency of two simple pooling strategies that can increase testing capacity about 5-fold to 7.5-fold, in populations with a low infection rate. We have implemented the method in a routine clinical diagnosis setting, and already tested 2,168 individuals for SARS-CoV-2 using 311 RNA extraction and RT-PCR kits.

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Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay

Abstract: 33

Cited by 126 publications

References 15 publications

Leveraging open hardware to alleviate the burden of COVID-19 on global health systems

Leveraging open hardware to alleviate the burden of COVID-19 on global health systems

Shotgun Transcriptome and Isothermal Profiling of SARS-CoV-2 Infection Reveals Unique Host Responses, Viral Diversification, and Drug Interactions

Large-scale implementation of pooled RNA-extraction and RT-PCR for SARS-CoV-2 detection

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