Rapid detection methods and modelling simulations provide new insights into cyanobacteria detection and bloom management in a tropical reservoir

Li, Han; Te, Shu Harn; Tavakoli, Yasaman; Zhang, J.; Gin, Karina Yew-Hoong; He, Yiliang

doi:10.1016/j.jenvman.2022.116730

Cited by 1 publication

(1 citation statement)

References 32 publications

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“…Insulated isothermal PCR (iiPCR) is a fluorescent hydrolysis probe-based technology that provides isothermal heating at the bottom of special capillary tubes to induce thermal convection and temperature gradient in an insulated environment to drive PCR reaction within a relatively short amount of time [ 22 , 23 ]. The POCKIT Nucleic Acid Analyzer series, which is a user-friendly iiPCR system (GeneReach Biotech), provide various iiPCR assays for detecting different human pathogens, including malaria, Zika virus, Middle East respiratory syndrome coronavirus (MERS-CoV), and dengue virus [ 24 , 25 , 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a Sample-to-Result POCKIT Central SARS-CoV-2 PCR System

Zhu

Tai²,

Ping³

et al. 2023

Diagnostics

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The emergence of COVID-19 has caused unprecedented impacts on global public health and many other aspects. Meanwhile, many types of methods have been developed to detect the causative agent, SARS-CoV-2; this has greatly advanced the technologies in the diagnostic field. Here, we describe the development and validation of a sample-in-result-out POCKIT Central SARS-CoV-2 PCR system for detecting SARS-CoV-2 in comparison with a commercial reference real-time RT-PCR assay (TaqPath COVID-19 Combo Kit). Both assays were specific and did not cross-react with non-SARS-CoV-2 agents. Both assays were able to detect various SARS-CoV-2 strains including some variants. Based on testing serial dilutions of SARS-CoV-2 USA-WA1/2020 isolate, the limit of detection was 0.8 TCID50/mL (1.87 × 103 genomic copies/mL) for POCKIT Central SARS-CoV-2 PCR and 0.16 TCID50/mL (3.75 × 102 genomic copies/mL) for the reference PCR. Subsequently, 183 clinical samples were tested by both assays and the diagnostic sensitivity, specificity, and agreement of the POCKIT Central SARS-CoV-2 PCR were 91.7%, 100%, and 94.0%, respectively, when compared to the reference PCR. The compact sample-to-result POCKIT Central SARS-CoV-2 PCR system is a simplified and efficient point-of-care tool for SARS-CoV-2 detection. In addition, this platform can be readily adapted to detect other human and animal viruses.

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