Rapid Detection and Assessment of Sparse Microbial Populations

Strange, R. E.

doi:10.1016/s0065-2911(08)60189-7

Cited by 6 publications

(2 citation statements)

References 119 publications

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“…A major problem in the application of ATP assays to quantitative bacteriology has been the frequent occurrence of large amounts of nonbacterial ATP emanating from cellular material present in many types of bacteriological specimens. If nonbacterial ATP cannot be removed prior to the assay, erroneous results will be obtained (13,14). The present study was performed to investigate the occurrence of nonbacterial ATP in urine and possible ways of its elimination.…”

mentioning

confidence: 99%

Detection of bacteriuria by luciferase assay of adenosine triphosphate

Thore¹,

Ånséhn²,

Lundin³

et al. 1975

J Clin Microbiol

148

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A selective method for distinguishing bacterial and nonbacterial adenosine triphosphate (ATP) in clinical bacteriological specimens was studied. The method involved incubation of samples with the detergent Triton X-100 and the ATP-hydrolyzing enzyme apyrase. The incubation selectively destroyed ATP in suspensions of various human cells while not affecting the ATP content in microbial cells. ATP remaining in the sample after incubation was extracted in boiling buffer and assayed by the firefly luciferase assay. Application of the method to 469 clinical urine specimens showed that the ATP level after treatment with Triton/apyrase was correlated to bacterial counts and that the sensitivity of the assay was sufficient for the detection of 105 bacteria/ml. The ATP levels per bacterial cell remaining in the urine specimens after treatment with Triton/apyrase were close to values observed in laboratory-grown cultures. The specificity and sensitivity of the luciferase assay for the detection of urinary bacteria and its possible use as a bacteriuria screening method are discussed.

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mentioning

confidence: 99%

Detection of bacteriuria by luciferase assay of adenosine triphosphate

Thore¹,

Ånséhn²,

Lundin³

et al. 1975

J Clin Microbiol

148

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“…When luminol (or myeloperoxidase) and hydrogen peroxide are added to bacterial cells, light is generated. As with bioluminescent reactions, the amount of light generated is proportional to the amount of iron-porphyrin compounds present and can be equated with bacterial concentration (4,132,193). This technique has been automated (138).…”

Section: Other Biophysical and Biochemical Approachesmentioning

confidence: 99%

New Methods for Microbiological Analysis of Food

Goldschmidt

Fung

1978

Journal of Food Protection

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This review article discusses the application and implications of automated or semi-automated instrumentation as well as miniaturized methods which can be used to detect and characterize microorganisms of importance in the food industry. The instrumentation section includes techniques involving turbidometry, radiometry, fluorometry, immunology, and chromatography. Miniaturized methods include various diagnostic kits and procedures. Instruments and techniques such as immobilized enzyme analysis and nuclear magnetic resonance, which have potential applications to this area of microbiology, are also mentioned.

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