Rapid design and fielding of four diagnostic technologies in Sierra Leone, Thailand, Peru, and Australia: Successes and challenges faced introducing these biosensors

Mulvaney, Shawn P.; Fitzgerald, Lisa A.; Hamdan, Leila J.; Ringeisen, Bradley R.; Petersen, Emily R.; Compton, Jaimee R.; McAuliff, Nina L.; Łęski, Tomasz A.; Taitt, Chris R.; Stenger, David A.; Myers, Christopher A.; Hansen, Erin; Ricketts, Michelle; Hoegberg, Chelsea; Homdayjanakul, Kamonthip; Ansumana, Rashid; Lamin, Joseph M.; Bangura, Umaru; Lahai, Joseph; Baio, Victoria; Limmathurotsakul, Direk; Wongsuvan, Gumphol; Hantrakun, Viriya; Wacharapluesadee, Supaporn; Mungaomklang, Anek; Putcharoen, Opass; Yatoom, Pratoomtong; Kruthakool, Kriengsak; Hontz, Robert D.; Mores, Christopher N.; Siles, Crystyan; Morrison, Amy C.; Mayo, Mark; Currie, Bart J.; Jacobsen, Kathryn H.; Quinn, Kathleen; Blutman, Jerold; Amariei, Flavia; Hannan, John R.

doi:10.1016/j.sbsr.2018.06.003

Cited by 8 publications

(7 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Depending on the assay and material tested, at least one malaria marker was detected in 36–61 % of the tested samples (Table 1 ). These results were in line with previous reports of malaria positivity among febrile individuals in this location as detected by PCR-based methods [ 18 , 19 ].…”

Section: Resultssupporting

confidence: 93%

Comparison of capillary and venous blood for malaria detection using two PCR-based assays in febrile patients in Sierra Leone

Łęski

Taitt

Bangura³

et al. 2021

Malar J

Self Cite

View full text Add to dashboard Cite

Background Rapid and sensitive diagnostics are critical tools for clinical case management and public health control efforts. Both capillary and venous blood are currently used for malaria detection and while diagnostic technologies may not be equally sensitive with both materials, the published data on this subject are scarce and not conclusive. Methods Paired clinical samples of venous and capillary blood from 141 febrile individuals in Bo, Sierra Leone, were obtained between January and May 2019 and tested for the presence of Plasmodium parasites using two multiplexed PCR assays: the FilmArray-based Global Fever Panel (GFP) and the TaqMan-based Malaria Multiplex Sample Ready (MMSR) assay. Results No significant differences in Plasmodium parasite detection between capillary and venous blood for both assays were observed. The GFP assay was more sensitive than MMSR for all markers that could be compared (Plasmodium spp. and Plasmodium falciparum) in both venous and capillary blood. Conclusions No difference was found in malaria detection between venous and capillary blood using two different PCR-based detection assays. This data gives support for use of capillary blood, a material which can be obtained easier by less invasive methods, for PCR-based malaria diagnostics, independent of the platform.

show abstract

Section: Resultssupporting

confidence: 93%

Comparison of capillary and venous blood for malaria detection using two PCR-based assays in febrile patients in Sierra Leone

Łęski

Taitt

Bangura³

et al. 2021

Malar J

Self Cite

View full text Add to dashboard Cite

show abstract

“…These sites were selected for their high dengue endemicity. Iquitos City (population ~470,000) [12, 23] located in the Peruvian Amazon rainforest, is geographically isolated and only accessible by plane or boat, and has been the site of several studies, primarily on dengue epidemiology, since 1999 by the University of California at Davis/U.S. Naval Medical Research Unit Six-Iquitos group (NAMRU-6) [17, 18, 24–28].…”

Section: Methodsmentioning

confidence: 99%

“…In 2012, the U.S. Defense Threat Reduction Agency Joint Science and Technology Office initiated a program to develop novel multiplex point-of-need (PON) diagnostics for surveillance of emerging infectious diseases. The first generation devices evaluated included ones that tested for dengue, malaria, plague, and melioidosis, integrated with a platform to automatically upload test results to a cloud-based biosurveillance system [12]. Evaluation in endemic countries was initiated in 2014 in sites in Thailand, Australia, Sierra Leone, Cambodia, and Peru to assess the use of these RDTs as close to the point-of-need as possible, including home-based testing by residents.…”

Section: Introductionmentioning

confidence: 99%

Potential Use of Community-Based Rapid Diagnostic Tests for Febrile Illnesses: Formative Research in Peru and Cambodia

et al. 2019

Self Cite

View full text Add to dashboard Cite

In 2012, the U.S. Defense Threat Reduction Agency Joint Science and Technology Office initiated a program to develop novel point-of-need diagnostic devices for surveillance of emerging infectious diseases including dengue, malaria, plague, and melioidosis. Prior to distribution of devices to observe their correct use among community members in Iquitos, Peru, and Phnom Penh, Cambodia, research was conducted to: 1) assess acceptability of use, including the motivation to use a rapid diagnostic test (RDT) before or instead of seeking care at a health facility, 2) explore comprehension of RDT use instructions, and 3) examine possible strategies for large scale RDT distribution and use at each site. In February 2014, 9 focus group discussions (FGD) with community members and 5 FGD with health professionals were conducted in Iquitos, and 9 FGD with community members and 9 in-depth interviews with health professionals in Phnom Penh. In both places, participants agreed to use the device themselves (involving finger prick) or could identify someone who could do so in their home or neighborhood. The main incentive to RDT use in both sites was the ability for device results to be used for care facilitation (post confirmatory tests), specifically reduced wait times to be seen or obtain a diagnosis. Comprehension of RDT use instructions was assessed in Iquitos by asking some participants to apply the device to research team members; after watching a short video, most steps were done correctly. In Phnom Penh, participants were asked to describe each step after reading the instructions; they struggled with comprehension. Health professionals’ main concerns in both sites were their community’s ability to accurately use the test, handle complicated instructions, and safety (i.e., disposal of lancets). Health system structure and ability to use home diagnostic devices varied in the two disease endemic sites, with substantial challenges in each, suggesting the need for different strategies for RDT large scale community use, and illustrating the value of formative research before deployment of novel technologies.

show abstract

“…The two prototype devices used in the observation study were under evaluation by DTRA [ 20 ], whose long-term goals were to develop multiplex devices to detect a wide array of pathogens including Biological Safety Level III/IV agents that could be applied directly by community members. Neither device was developed further, but the DTRA-funded observation studies were for these devices, which provided us a model system to test devices of varied complexity.…”

Section: Methodsmentioning

confidence: 99%

Potential for community based surveillance of febrile diseases: Feasibility of self-administered rapid diagnostic tests in Iquitos, Peru and Phnom Penh, Cambodia

et al. 2021

Self Cite

View full text Add to dashboard Cite

Rapid diagnostic tests (RDTs) have the potential to identify infectious diseases quickly, minimize disease transmission, and could complement and improve surveillance and control of infectious and vector-borne diseases during outbreaks. The U.S. Defense Threat Reduction Agency’s Joint Science and Technology Office (DTRA-JSTO) program set out to develop novel point-of-need RDTs for infectious diseases and deploy them for home use with no training. The aim of this formative study was to address two questions: 1) could community members in Iquitos, Peru and Phnom Penh, Cambodia competently use RDTs of different levels of complexity at home with visually based instructions provided, and 2) if an RDT were provided at no cost, would it be used at home if family members displayed febrile symptoms? Test kits with written and video (Peru only) instructions were provided to community members (Peru [n = 202]; Cambodia [n = 50]) or community health workers (Cambodia [n = 45]), and trained observers evaluated the competency level for each of the several steps required to successfully operate one of two multiplex RDTs on themselves or other consenting participant (i.e., family member). In Iquitos, >80% of residents were able to perform 11/12 steps and 7/15 steps for the two- and five-pathogen test, respectively. Competency in Phnom Penh never reached 80% for any of the 12 or 15 steps for either test; the percentage of participants able to perform a step ranged from 26–76% and 23–72%, for the two- and five-pathogen tests, respectively. Commercially available NS1 dengue rapid tests were distributed, at no cost, to households with confirmed exposure to dengue or Zika virus; of 14 febrile cases reported, six used the provided RDT. Our findings support the need for further implementation research on the appropriate level of instructions or training needed for diverse devices in different settings, as well as how to best integrate RDTs into existing local public health and disease surveillance programs at a large scale.

show abstract

Rapid design and fielding of four diagnostic technologies in Sierra Leone, Thailand, Peru, and Australia: Successes and challenges faced introducing these biosensors

Cited by 8 publications

References 26 publications

Comparison of capillary and venous blood for malaria detection using two PCR-based assays in febrile patients in Sierra Leone

Comparison of capillary and venous blood for malaria detection using two PCR-based assays in febrile patients in Sierra Leone

Potential Use of Community-Based Rapid Diagnostic Tests for Febrile Illnesses: Formative Research in Peru and Cambodia

Potential for community based surveillance of febrile diseases: Feasibility of self-administered rapid diagnostic tests in Iquitos, Peru and Phnom Penh, Cambodia

Contact Info

Product

Resources

About