Rapid degradation of starch in chloroplasts and concomitant accumulation of soluble sugars associated with ABA-induced freezing tolerance in the moss Physcomitrella patens

Nagao, Manabu; Minami, Anzu; Arakawa, Keita; Fujikawa, Seizo; Takezawa, Daisuke

doi:10.1016/j.jplph.2004.06.012

Cited by 114 publications

(95 citation statements)

References 35 publications

(42 reference statements)

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“…The results of both scanning electron microscopy and semisectioning with embedded resin indicated that the anatomical structure of both the stolons and leaves changed with increasing nutrient levels. It is well known that stress can result in plants that are unhealthy and poorly developed, and associated physiological and anatomical changes include reduced stomatal aperture (Chartzoulakis et al, 1999), the degradation of starch (Goyal et al, 1986;Lloyd et al, 2005) and a reduction of the number of chloroplasts (Nagao et al, 2005). Aerenchyma, a spongy tissue with large air spaces, provides a low-resistance internal pathway for the storage and exchange of gases within plant tissues (Visser et al, 1997;López-Bucio et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Ecophysiological and anatomical responses ofVallisneria natansto nitrogen and phosphorus enrichment

Wang

Gao

Qin

et al. 2012

Knowl. Managt. Aquatic Ecosyst.

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Here, we describe an experiment using four nitrogen (N) and phosphorus (P) concentrations to investigate the effects of nutrient enrichment on the submersed macrophyte Vallisneria natans (tape grass) grown in a sand culture medium. The objective of this study was to examine the influence of nutrient enrichment in the water column on V. natans, especially with regard to anatomical structures. The results showed both the absolute growth rate (AGR) and intrinsic efficiency of light energy conversion of PSII (Fv/Fm) decreased with increasing nutrient levels. Root morphological characteristics, including the total root length (L), root surface area (SA), projected root area (PA), total root volume (V), average root diameter (AD), total root length per volume (LPV), total tips (T) and total forks (F), also showed a generally negative relationship with increasing nutrient concentrations. The anatomical structures of stolons and leaves also changed with nutrient enrichment. The shrinkage of aerenchyma and disappearance of starches and chloroplasts were the main structural changes leading to poor growth. These phenomena, especially the anatomical changes, might be the mechanism underlying the effect of nutrient enrichment on V. natans growth. Nous décrivons ici une expérience utilisant quatre concentrations d'azote (N) et phosphore (P) afin d'étudier les effets de l'enrichissement en éléments nutritifs sur le macrophyte immergé Vallisneria natans cultivé dans un milieu de culture sableux. L'objectif de cette étude était d'examiner l'influence de l'enrichissement en nutriments dans la colonne d'eau sur V. natans, en particulier en ce qui concerne les structures anatomiques. Les résultats ont montré à la fois que le taux de croissance absolue (AGR) et l'efficacité intrinsèque de la conversion de l'énergie lumineuse du PSII (Fv/Fm) ont diminué avec l'augmentation des niveaux d'éléments nutritifs. Les caractéristiques morphologiques racinaires, y compris la longueur totale des racines (L), la surface des racines (SA), la zone des racines prospectée (PA), le volume total des racines (V), le diamètre moyen des racines (AD), la longueur totale des racines par volume (LPV), le totalité des extrémités (T) et des ramifications (F), ont également montré une relation globalement négative avec RÉSUMÉ Réponses écophysiologiques et anatomiques de Vallisneria natans à un enrichissement en azote et en phosphore Mots

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Section: Discussionmentioning

confidence: 99%

Ecophysiological and anatomical responses ofVallisneria natansto nitrogen and phosphorus enrichment

Wang

Gao

Qin

et al. 2012

Knowl. Managt. Aquatic Ecosyst.

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“…P. patens is dehydrationtolerant but can become desiccation-and freezing-tolerant by pre-treatment with ABA (Khandelwal et al 2010;Nagao et al 2005). Whereas abiotic stress signaling seems largely conserved between mosses and flowering plants (Khandelwal et al 2010;Komatsu et al 2013;Richardt et al 2010), several studies report on so-called orphan, non-characterized stress responsive moss genes and proteins (Beike et al 2015b;Cui et al 2012).…”

Section: Mosses Employ Several Strategies To Increase Stress Tolerancementioning

confidence: 99%

Can mosses serve as model organisms for forest research?

Müller

Gütle

Jacquot

et al. 2016

Annals of Forest Science

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& Key message Based on their impact on many ecosystems, we review the relevance of mosses in research regarding stress tolerance, metabolism, and cell biology. We introduce the potential use of mosses as complementary model systems in molecular forest research, with an emphasis on the most developed model moss Physcomitrella patens. & Context and aims Mosses are important components of several ecosystems. The moss P. patens is a well-established nonvascular model plant with a high amenability to molecular biology techniques and was designated as a JGI plant flagship genome. In this review, we will provide an introduction to moss research and highlight the characteristics of P. patens and other mosses as a potential complementary model system for forest research. & Methods Starting with an introduction into general moss biology, we summarize the knowledge about moss physiology and differences to seed plants. We provide an overview of the current research areas utilizing mosses, pinpointing potential links to tree biology. To complement literature review, we discuss moss advantages and available resources regarding molecular biology techniques. & Results and conclusion During the last decade, many fundamental processes and cell mechanisms have been studied in mosses and seed plants, increasing our knowledge of plant evolution. Additionally, moss-specific mechanisms of stress tolerance are under investigation to understand their resilience in ecosystems. Thus, using the advantages of model mosses such as P. patens is of high interest for various research approaches, including stress tolerance, organelle biology, cell polarity, and secondary metabolism.

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“…Ethidium bromidestained rRNA for each sample is also shown. the cells are damaged by freezing, from 22°C to 210°C in protonemata of P. patens (Minami et al, 2003;Nagao et al, 2005). Thus, ABA-treated protonemata of nontransgenic and transgenic moss lines were subjected to freezing to 25°C, and after thawing, survival was determined by regrowth on agar medium.…”

Section: Changes In Osmotic and Freezing Stress Tolerance In Transgenmentioning

confidence: 99%

“…Suspension culture of M. polymorpha was maintained in the M51C liquid medium containing 2% Suc and 1 mg L 21 2,4-dichlorophenoxyacetic acid at 22°C with shaking at 130 rpm (Ono et al, 1979). Protonemata of Physcomitrella patens were cultured using the modified BCD agar medium as described by Nagao et al (2005).…”

Section: Plant Materialsmentioning

confidence: 99%

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Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

et al. 2010

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Abscisic acid (ABA) is postulated to be a ubiquitous hormone that plays a central role in seed development and responses to environmental stresses of vascular plants. However, in liverworts (Marchantiophyta), which represent the oldest extant lineage of land plants, the role of ABA has been least emphasized; thus, very little information is available on the molecular mechanisms underlying ABA responses. In this study, we isolated and characterized MpABI1, an ortholog of ABSCISIC ACID INSENSITIVE1 (ABI1), from the liverwort Marchantia polymorpha. The MpABI1 cDNA encoded a 568-amino acid protein consisting of the carboxy-terminal protein phosphatase 2C (PP2C) domain and a novel amino-terminal regulatory domain. The MpABI1 transcript was detected in the gametophyte, and its expression level was increased by exogenous ABA treatment in the gemma, whose growth was strongly inhibited by ABA. Experiments using green fluorescent protein fusion constructs indicated that MpABI1 was mainly localized in the nucleus and that its nuclear localization was directed by the aminoterminal domain. Transient overexpression of MpABI1 in M. polymorpha and Physcomitrella patens cells resulted in suppression of ABA-induced expression of the wheat Em promoter fused to the b-glucuronidase gene. Transgenic P. patens expressing MpABI1 and its mutant construct, MpABI1-d2, lacking the amino-terminal domain, had reduced freezing and osmotic stress tolerance, and associated with reduced accumulation of ABA-induced late embryogenesis abundant-like boiling-soluble proteins. Furthermore, ABA-induced morphological changes leading to brood cells were not prominent in these transgenic plants. These results suggest that MpABI1 is a negative regulator of ABA signaling, providing unequivocal molecular evidence of PP2C-mediated ABA response mechanisms functioning in liverworts.Land plants are repeatedly challenged by environmental stresses such as desiccation, salinity, and freezing, which can cause irreversible damage to intracellular structures and membranes by severe dehydration. Abscisic acid (ABA), which is known to be a phytohormone responsible for physiological control of seed maturation and germination, bud dormancy, and stomata closure, has been postulated to be involved in the development of cellular dehydration tolerance under stress conditions. In higher plants, water-stress conditions trigger a transient or sustained increase in endogenous ABA content in tissues. Increased ABA stimulates the expression of a number of stress-related genes such as those of late embryogenesis abundant (LEA) proteins, which are thought to protect cells from the damage caused by the dehydration stress (Chandler and Robertson, 1994). It has been demonstrated that various tissue-cultured cells of vascular plants, including those of angiosperms, gymnosperms, and ferns, develop desiccation and freezing tolerance upon exogenous ABA treatment, indicating that ABA plays a key role in stress tolerance at cellular

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Rapid degradation of starch in chloroplasts and concomitant accumulation of soluble sugars associated with ABA-induced freezing tolerance in the moss Physcomitrella patens

Cited by 114 publications

References 35 publications

Ecophysiological and anatomical responses ofVallisneria natansto nitrogen and phosphorus enrichment

Ecophysiological and anatomical responses ofVallisneria natansto nitrogen and phosphorus enrichment

Can mosses serve as model organisms for forest research?

Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

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