Rapid Cycle Breeding: Application of Transgenic Early Flowering for Perennial Trees

Callahan, Ann M.; Srinivasan, C.; Dardick, Chris; Scorza, R.

doi:10.1002/9781119279723.ch7

Cited by 13 publications

(8 citation statements)

References 111 publications

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“…In contrast, the situation in the United States is completely different. There, null-segregants obtained by the rapid cycle breeding approach can be freely planted in the open field without any restrictions ( Callahan et al, 2016 ). Transgenic plants used for crosses can also be planted in the field, as long as they are netted ( Figure 5 ).…”

Section: Rapid Cycle Breedingmentioning

confidence: 99%

Recent Developments and Strategies for the Application of Agrobacterium-Mediated Transformation of Apple Malus × domestica Borkh

et al. 2022

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Genetic transformation has become an important tool in plant genome research over the last three decades. This applies not only to model plants such as Arabidopsis thaliana but also increasingly to cultivated plants, where the establishment of transformation methods could still pose many problems. One of such plants is the apple (Malus spp.), the most important fruit of the temperate climate zone. Although the genetic transformation of apple using Agrobacterium tumefaciens has been possible since 1989, only a few research groups worldwide have successfully applied this technology, and efficiency remains poor. Nevertheless, there have been some developments, especially in recent years, which allowed for the expansion of the toolbox of breeders and breeding researchers. This review article attempts to summarize recent developments in the Agrobacterium-mediated transformation strategies of apple. In addition to the use of different tissues and media for transformation, agroinfiltration, as well as pre-transformation with a Baby boom transcription factor are notable successes that have improved transformation efficiency in apple. Further, we highlight targeted gene silencing applications. Besides the classical strategies of RNAi-based silencing by stable transformation with hairpin gene constructs, optimized protocols for virus-induced gene silencing (VIGS) and artificial micro RNAs (amiRNAs) have emerged as powerful technologies for silencing genes of interest. Success has also been achieved in establishing methods for targeted genome editing (GE). For example, it was recently possible for the first time to generate a homohistont GE line into which a biallelic mutation was specifically inserted in a target gene. In addition to these methods, which are primarily aimed at increasing transformation efficiency, improving the precision of genetic modification and reducing the time required, methods are also discussed in which genetically modified plants are used for breeding purposes. In particular, the current state of the rapid crop cycle breeding system and its applications will be presented.

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Section: Rapid Cycle Breedingmentioning

confidence: 99%

Recent Developments and Strategies for the Application of Agrobacterium-Mediated Transformation of Apple Malus × domestica Borkh

et al. 2022

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“…As a tool, this will allow more efficient in vivo functional analysis of cacao genes, subcellular localization of proteins and promoter analysis. It is also possible that the use of rapidly flowering transgenic lines, as used in other perennial species (Callahan et al ., ), may be applicable to cacao. Most recently, an inducible SE system in cacao has been developed by use of a transgenic plant expressing the LEC2 embryogenic transcription factor (Shires et al ., ).…”

Section: Genetic Transformationmentioning

confidence: 99%

Cacao biotechnology: current status and future prospects

Wickramasuriya

Dunwell

2017

Plant Biotechnology Journal

105

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Summary Theobroma cacao—The Food of the Gods, provides the raw material for the multibillion dollar chocolate industry and is also the main source of income for about 6 million smallholders around the world. Additionally, cocoa beans have a number of other nonfood uses in the pharmaceutical and cosmetic industries. Specifically, the potential health benefits of cocoa have received increasing attention as it is rich in polyphenols, particularly flavonoids. At present, the demand for cocoa and cocoa‐based products in Asia is growing particularly rapidly and chocolate manufacturers are increasing investment in this region. However, in many Asian countries, cocoa production is hampered due to many reasons including technological, political and socio‐economic issues. This review provides an overview of the present status of global cocoa production and recent advances in biotechnological applications for cacao improvement, with special emphasis on genetics/genomics, in vitro embryogenesis and genetic transformation. In addition, in order to obtain an insight into the latest innovations in the commercial sector, a survey was conducted on granted patents relating to T. cacao biotechnology.

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“…Otra ventaja importante de la inducción biotecnológica de la floración, además de la reducción significativa del tiempo necesario en el proceso de mejoramiento genético vegetal, es la posibilidad de usarla en el proceso y no en el producto; es decir, segregar afuera, a través de las cruzas, el transgen necesario para inducir la floración y de esta manera no incluirlo en el producto (Callahan et al, 2016).…”

Section: El Uso De La Estrategia De Inducción De Floración En Ciruelaunclassified

Mejoramiento genético acelerado de angiospermas perennes vía inducción floral por sobre-expresión del gen FT

Urrea-López¹

2018

RMCF

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A.C. Unidad Noreste. México. Correo-e: rafaelurrea80@yahoo.es ResumenLos bosques y selvas enfrentan el reto de satisfacer la demanda por recursos de una población en crecimiento, así como la amenaza del rápido cambio climático que exacerba la magnitud y frecuencia de estreses bióticos y abióticos. Para ello, es urgente acelerar el mejoramiento genético de especies forestales. Sin embargo, sus largas etapas juveniles y asincronía floral retrasan peligrosamente este proceso. El presente ensayo explora los adelantos biotecnológicos en inducción floral y su potencial aplicación en especies forestales. Entre los genes identificados y caracterizados que participan en la ruta de señalización de la floración, especial atención se destina al gen FLOWERING LOCUS T, considerado un integrador de rutas de señalización altamente conservado entre las angiospermas, que, al sobre-expresarse por ingeniería genética, es capaz de inducir la floración de forma eficiente. Esta novedosa estrategia biotecnológica se ha utilizado, recientemente, para segregar genes de resistencia a enfermedades, en un menor tiempo, en germoplasma comercial de manzana y ciruela. Permite soslayar barreras naturales que por mucho tiempo han restringido a las especies forestales al mejoramiento por selección, principalmente. Entre sus ventajas está la de poder restringirla al proceso y no al producto, para acelerar las cruzas sexuales sin modificar genéticamente la progenie; se aleja así de la controversia alrededor de la liberación y consumo de organismos genéticamente modificados, y de los costos y trámites obligatorios para los OGM para monitoreo de posibles riesgos. Se proyecta como una tecnología que puede acelerar, significativamente, el mejoramiento de especies forestales.Palabras clave: Biotecnología forestal, FLOWERING LOCUS T, ingeniería genética vegetal, mejoramiento acelerado de perennes, mejoramiento de árboles, inducción de floración.

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Rapid Cycle Breeding: Application of Transgenic Early Flowering for Perennial Trees

Cited by 13 publications

References 111 publications

Recent Developments and Strategies for the Application of Agrobacterium-Mediated Transformation of Apple Malus × domestica Borkh

Recent Developments and Strategies for the Application of Agrobacterium-Mediated Transformation of Apple Malus × domestica Borkh

Cacao biotechnology: current status and future prospects

Mejoramiento genético acelerado de angiospermas perennes vía inducción floral por sobre-expresión del gen FT

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