Rapid Colorimetric Determination of Procalcitonin Using Magnetic Separation and Enzymatic Catalysis

Zhang, Yun; Si, Xiaohui; Zhang, Miaomiao; Yang, Xue; Yuan, Huan; Wang, Xiangpeng; Zhang, Yang; Wang, Hui

doi:10.1080/00032719.2018.1482312

Cited by 11 publications

(10 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the potential of immunosensors based on magnetic beads has not been widely explored for PCT determination, which is apparent in the low number of publications (Table 4). In comparison with those works, our SPE-C approach offers enough sensitivity (similar to our previous work [40]) to perform reliable PCT detection, but with a significant reduction in the analysis time and sample volumes [16,17,35,36]. Moreover, the easy automation of the electrochemical transduction and the improved sensitivity [40] bring our new approach (on-chip EMC-Au) closer to the POC concept.…”

Section: Analysis Of Human Serum and Plasma Samplesmentioning

confidence: 73%

“…Several methods have been reported for PCT determination, mainly based in the selective recognition provided by immunological interactions. These methodologies include immunoturbidimetric assays [9], chemiluminescent immunoassays [10][11][12][13][14][15][16], immunochromatographic assays [17][18][19][20][21][22], surface plasmon resonance biosensors [23][24][25], fluorescence immunosensors [26][27][28][29][30][31][32], ellipsometry immunosensors [33], colorimetric immunoassays [34][35][36], and electrochemical immunoassays [37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56]. Even differen...…”

Section: Introductionmentioning

confidence: 99%

“…The use of magnetic beads has been largely exploited in recent years due to their well-known properties such as improved assay kinetics and easy manipulation, while they are perfectly coupled to electrochemical detection [58]. However, magneto-immunosensors [16,17,35,36] in general, and particularly those using electrochemical detection [40], have not been widely explored for PCT determination.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Magnetic Bead-Based Electrochemical Immunoassays On-Drop and On-Chip for Procalcitonin Determination: Disposable Tools for Clinical Sepsis Diagnosis

et al. 2020

View full text Add to dashboard Cite

Procalcitonin (PCT) is a known protein biomarker clinically used for the early stages of sepsis diagnosis and therapy guidance. For its reliable determination, sandwich format magnetic bead-based immunoassays with two different electrochemical detection approaches are described: (i) disposable screen-printed carbon electrodes (SPE-C, on-drop detection); (ii) electro-kinetically driven microfluidic chips with integrated Au electrodes (EMC-Au, on-chip detection). Both approaches exhibited enough sensitivity (limit of detection (LOD) of 0.1 and 0.04 ng mL−1 for SPE-C and EMC-Au, respectively; cutoff 0.5 ng mL−1), an adequate working range for the clinically relevant concentrations (0.5–1000 and 0.1–20 ng mL−1 for SPE-C and EMC-Au, respectively), and good precision (RSD < 9%), using low sample volumes (25 µL) with total assay times less than 20 min. The suitability of both approaches was successfully demonstrated by the analysis of human serum and plasma samples, for which good recoveries were obtained (89–120%). Furthermore, the EMC-Au approach enabled the easy automation of the process, constituting a reliable alternative diagnostic tool for on-site/bed-site clinical analysis.

show abstract

Section: Analysis Of Human Serum and Plasma Samplesmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Magnetic Bead-Based Electrochemical Immunoassays On-Drop and On-Chip for Procalcitonin Determination: Disposable Tools for Clinical Sepsis Diagnosis

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In summary, accurate detection of PCT content in serum has important significance for the early prevention and further treatment of inflammation [ 11 , 12 ]. To date, many PCT detection strategies have been reported, including colorimetric immunoassay [ 13 , 14 ], chemiluminescence immunoassay [ 15 ], electrochemical immunoassay [ 16 ], microfluidic immunoassay, and fluorescence immunoassay [ 17 , 18 ]. Here, we studied the application of PEC immunosensor technology to detect PCT.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Photoelectrochemical Immunoassay Strategy Based on Bi2S3/Ag2S for the Detection of the Inflammation Marker Procalcitonin

Zhao

Wang

et al. 2023

Biosensors

View full text Add to dashboard Cite

As an inflammatory marker, procalcitonin (PCT) is more representative than other traditional inflammatory markers. In this work, a highly efficient photoelectrochemical (PEC) immunosensor was constructed based on the photoactive material Bi2S3/Ag2S to realize the sensitive detection of PCT. Bi2S3 was prepared by a hydrothermal method, and Ag2S quantum dots were deposited on the ITO/Bi2S3 surface via in situ reduction. Bi2S3 is a kind of admirable photoelectric semiconductor nanomaterial on account of its moderate bandgap width and low binding rate of photogenerated electron holes, which can effectively convert light energy into electrical energy. Therefore, based on the energy level matching principle of Bi2S3 and Ag2S, a labeled Bi2S3/Ag2S PEC immunosensor was constructed, and the sensitive detection of PCT was successfully established. The linear detection range of the PEC immunosensor was 0.50 pg∙mL−1 to 50 ng∙mL−1, and the minimum detection limit was 0.18 pg∙mL−1. Compared with the traditional PEC strategy, the proposed PEC immunosensor is simple, convenient, and has good anti-interference, sensitivity, and specificity, which could provide a meaningful theoretical basis and reference value for the clinical detection of PCT.

show abstract

“…Currently, a wide range of approaches have been developed for determination of PCT, including immunoturbidimetric assay, chemiluminescent immunoassay, − immunochromatographic assay, − surface plasmon resonance biosensor, − electrochemical immunosensor, − ellipsometry immunosensor, colorimetric immunoassays, − and fluorescence immunoassay (FIA). − Although some of them reach impressive sensitivity, these methods present several limitations, such as their high complexity, sophisticated instrumentation requirements, and/or unproven applicability in the real clinical scenario. Neonatal sepsis diagnosis poses a particular challenge because of difficulties in obtaining blood samples or limited volume availability.…”

mentioning

confidence: 99%

Polymer-Based Micromotor Fluorescence Immunoassay forOn-the-MoveSensitive Procalcitonin Determination in Very Low Birth Weight Infants’ Plasma

et al. 2020

View full text Add to dashboard Cite

A new fluorescence micromotor-based immunoassay (FMIm) has been developed for procalcitonin (PCT) determination as an early sepsis diagnostic analytical tool. The micromotors combine the high binding capacity of the specific antibodies onto their polymeric polypyrrole outer layer (PPy layer), with their magnetic guidance (Ni layer) and self-propulsion by catalytic generation of oxygen bubbles (PtNP inner layer) to actively recognize the PCT antigen. This FMIm allowed a sensitive (LOD = 0.07 ng mL −1 ) and direct PCT determination in clinical samples from very lowbirth-weight infants (VLBWI) with sepsis suspicion, using small volumes of sample (25 μL) in a clinically relevant range of concentrations (0.5−150 ng mL −1 ). The good agreement between PCT levels obtained by our micromotor-based method and routine immunofluorescence hospital determination demonstrates the feasibility for the analysis in VLBWI samples and its potential as a point-of-care diagnostic tool for sepsis.

show abstract

Rapid Colorimetric Determination of Procalcitonin Using Magnetic Separation and Enzymatic Catalysis

Cited by 11 publications

References 34 publications

Magnetic Bead-Based Electrochemical Immunoassays On-Drop and On-Chip for Procalcitonin Determination: Disposable Tools for Clinical Sepsis Diagnosis

Magnetic Bead-Based Electrochemical Immunoassays On-Drop and On-Chip for Procalcitonin Determination: Disposable Tools for Clinical Sepsis Diagnosis

Ultrasensitive Photoelectrochemical Immunoassay Strategy Based on Bi2S3/Ag2S for the Detection of the Inflammation Marker Procalcitonin

Polymer-Based Micromotor Fluorescence Immunoassay forOn-the-MoveSensitive Procalcitonin Determination in Very Low Birth Weight Infants’ Plasma

Contact Info

Product

Resources

About