Rapid clearance of procoagulant platelet‐derived microparticles from the circulation of rabbits

Rand, Margaret L.; Wang, H.; Bang, K.W. Annie; Packham, Marian A.; Freedman, John

doi:10.1111/j.1538-7836.2006.02011.x

Cited by 92 publications

(72 citation statements)

References 21 publications

(25 reference statements)

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“…Phosphatidylserine-expressing platelet microparticles have a short half-life of Ͻ10 minutes when infused, which implies that microparticles are continuously formed in the circulation. 37 The mechanism of clearance of platelet microparticles from the circulation is not well understood; some clues may be obtained from studies on the clearance of phospholipid liposomes, which have been used extensively as vehicles for drug delivery. 38 The clearance of liposomes is affected markedly by the lipid composition of the liposomes; phosphatidylserine markedly enhances the clearance rate of liposomes.…”

Section: Discussionmentioning

confidence: 99%

Developmental Endothelial Locus-1 (Del-1) Mediates Clearance of Platelet Microparticles by the Endothelium

et al. 2012

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Background-Phosphatidylserine-expressing microparticles circulate in blood with a short half-life of Ͻ10 minutes. We tested the role of an endothelium-derived phosphatidylserine-binding opsonin, developmental endothelial locus-1 (Del-1), in the uptake of platelet microparticles. Methods and Results-Cultured human umbilical vein and microvascular endothelial cells avidly engulf BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)-maleimide-labeled platelet microparticles. Microparticle uptake was inhibited by a monoclonal antibody to Del-1 (Pϭ0.027) and by annexin A5 (Pϭ0.027), abciximab (Pϭ0.027), a monoclonal antibody to integrin ␣V␤3 (Pϭ0.027), and chlorpromazine (Pϭ0.027). These results suggest that Del-1 mediates phosphatidylserine-and integrin-dependent endothelial uptake of microparticles by endocytosis. To assess the in vivo significance, we infused fluorescent platelet microparticles into the inferior vena cava of mice and harvested endothelial cells from the pulmonary and systemic circulation. Compared with their wild-type littermates, Del-1-deficient mice had decreased uptake in endothelial cells in lung (3.07Ϯ1.9 versus 1.09Ϯ1.

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Section: Discussionmentioning

confidence: 99%

Developmental Endothelial Locus-1 (Del-1) Mediates Clearance of Platelet Microparticles by the Endothelium

et al. 2012

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“…Microparticle levels depend on speed of clearance mechanism, currently thought to involve direct receptor binding of liver or spleen phagocytes to phosphatidylserine or opsonization proteins on the microparticles. 17,18 Platelet-derived microparticle levels are known to fall rapidly after injection into rabbits 19 and within 30 minutes after infusion in mice, 17 but few studies have investigated human clearance. 20 We found that, within 1 hour, elevated circulating procoagulant, platelet, and EMPs are significantly reduced.…”

Section: Discussionmentioning

confidence: 99%

Dynamic Release and Clearance of Circulating Microparticles During Cardiac Stress

Augustine

Ayers

Lima

et al. 2014

Circulation Research

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Rationale: Microparticles are cell-derived membrane vesicles, relevant to a range of biological responses and known to be elevated in cardiovascular disease. Objective: To investigate microparticle release during cardiac stress and how this response differs in those with vascular disease. Methods and Results: We measured a comprehensive panel of circulating cell-derived microparticles by a standardized flow cytometric protocol in 119 patients referred for stress echocardiography. Procoagulant, platelet, erythrocyte, and endothelial but not leukocyte, granulocyte, or monocyte-derived microparticles were elevated immediately after a standardized dobutamine stress echocardiogram and decreased after 1 hour. Twenty-five patients developed stress-induced wall motion abnormalities suggestive of myocardial ischemia. They had similar baseline microparticle levels to those who did not develop ischemia, but, interestingly, their microparticle levels did not change during stress. Furthermore, no stress-induced increase was observed in those without inducible ischemia but with a history of vascular disease. Fourteen patients subsequently underwent coronary angiography. A microparticle rise during stress echocardiography had occurred only in those with normal coronary arteries. Conclusions: Procoagulant, platelet, erythrocyte, and endothelial microparticles are released during cardiac stress and then clear from the circulation during the next hour. This stress-induced rise seems to be a normal physiological response that is diminished in those with vascular disease.

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“…Appropriate quality control procedures were followed; the flow cytometer settings were identical for all analyses and calibrations with standard-sized beads were done routinely. Single platelets and plateletderived microparticles (MPs) were identified by their characteristic forward light scatter and high CD41 or CD61 fluorescences; MPs were defined as events <0.75 lm polystyrene particles [30] for controls and BSS patients. A minimum of 10,000 events was acquired on each sample and analyzed using FlowJo software (Tree Star, Ashland, OR).…”

mentioning

confidence: 99%

Phosphatidylserine exposure and other apoptotic‐like events in Bernard‐Soulier syndrome platelets

et al. 2010

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In the Bernard-Soulier syndrome (BSS), the giant platelets are said to have increased phosphatidylserine (PS) surface exposure in the resting state and shortened survival in the circulation. When normal platelets are activated, they undergo many biochemical and morphological changes, some of which are apoptotic. Herein, we investigated apoptotic-like events in BSS platelets upon activation, specifically, PS exposure, microparticle (MP) formation, cell shrinkage, and loss of mitochondrial inner membrane potential (DC m ). Platelets from two unrelated BSS patients were examined in whole blood; agonists used were collagen, thrombin, PAR1-or PAR4-activating peptides (APs), or combinations of collagen with thrombin, and the PAR-APs. Flow cytometry was used to measure PS exposure (annexin A5 binding), platelet-derived MPs (forward scatter; events <0.75 lm size), and DC m (TMRM fluorescence). PS exposure was increased on resting and activated BSS platelets, and this was independent of the platelet size. MP formation by BSS platelets was generally enhanced. Cell shrinkage occurred on activation to form smaller, PS-exposing platelets in BSS and controls. A proportion of PS-exposing BSS and control platelets exhibited DC m loss, but unlike controls, there was also loss of DC m in the BSS platelets not exposing PS. Thus, BSS platelets undergo apoptotic-like events upon activation, with PS exposure and MP formation being enhanced. These events may play a role in the shortened survival in BSS, as well as affecting thrombin generation. Am. J. Hematol. 85:584-592, 2010. V

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Rapid clearance of procoagulant platelet‐derived microparticles from the circulation of rabbits

Cited by 92 publications

References 21 publications

Developmental Endothelial Locus-1 (Del-1) Mediates Clearance of Platelet Microparticles by the Endothelium

Developmental Endothelial Locus-1 (Del-1) Mediates Clearance of Platelet Microparticles by the Endothelium

Dynamic Release and Clearance of Circulating Microparticles During Cardiac Stress

Phosphatidylserine exposure and other apoptotic‐like events in Bernard‐Soulier syndrome platelets

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