Rapid Chemical and Biological Techniques for Water Monitoring

Gonzalez, Catherine; Greenwood, R. C.; Quevauviller, Philippe

doi:10.1002/9780470745427

Cited by 14 publications

(1 citation statement)

References 30 publications

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“…Water is being utilised by all life forms in various industries including food manufacturing and leisure industries. Yet, the accessibility of excellent water sources is gradually worsened as it is becoming polluted over time (Ahmad et al, 2016a;Gonzalez et al, 2009). The incorporation of noxious wastes such as heavy metals and pesticides into the ecosystem, deriving from multiple agrarian and industrial activities can produce alarming effects (Fatima et al, 2014;Sabullah et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Cholinesterase from the Liver of Diodon hystrix for Detection of Metal Ions

Nordin¹,

Cletus²,

Sabullah³

et al. 2020

JST

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The discharge of industrial effluents into nearby water bodies affects the inhabitants including living organisms. The presence of foreign materials such as heavy metals can be a threat to the ecosystem as they are enormously carcinogenic even though in minute concentration. Hence, an economical and time-efficient preliminary screening test is crucial to be developed for the detection of heavy metals, prior to employment of high technology instruments. In this study, cholinesterase (ChE) from Sabah porcupine fish, Diodon hystrix was purified to test for its potential as an alternative biosensor in detecting metal ions. Few enzymatic parameters including specificity of substrate, temperature and pH were applied to determine its optimal enzymatic activity. ChE enzyme was found to be more sensitive towards the presence of substrate, butyrylthiocholine iodide (BTC), in contrast to acetylthiocholine iodide (ATC) and propionylthiocholine iodide (PTC) with the effective coefficient at 7193, 3680.15 and 2965.26 Vmax/Km, respectively. Moreover, the extracted ChE enzyme showed the optimum activity at pH 9 of 0.1 M Tris-HCl and at 25°C to 30°C range of temperature. When subjected to heavy metals, ChE enzyme was significantly inhibited as the enzyme activity was reduced in the sequence of Hg > Ag > Cr > Cu > Cd > Pb ≥ Zn > As. As a conclusion, the partially purified ChE enzyme proved its sensitivity towards metal ion exposure and can be used as an alternative method in screening the level of contamination in the environment.

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Section: Introductionmentioning

confidence: 99%

Cholinesterase from the Liver of Diodon hystrix for Detection of Metal Ions

Nordin¹,

Cletus²,

Sabullah³

et al. 2020

JST

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The Effect of Temperature Factor on the Detection of Nitrate Based on Planar Electromagnetic Sensor and Independent Component Analysis

Yunus

Mukhopadhyay

Punchihewa

et al. 2012

Lecture Notes in Electrical Engineering

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Whole-cell aquatic biosensors

2010

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The use of various whole-cell organisms as tools for monitoring water contaminants is reviewed and evaluated. Their suitability and value for assessing a range of environmental problems from the impact of pollution, homeland defense issues, conservation, and the long-term degradation and recovery of ecosystems is discussed. Guidelines are provided for the choice of appropriate bioreporter organisms, transducers, and immobilization methods. Examples of the use of a broad spectrum of whole-cell bioreporters in assessing a variety of environmental problems are summarized.

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Rapid Chemical and Biological Techniques for Water Monitoring

Cited by 14 publications

References 30 publications

Cholinesterase from the Liver of Diodon hystrix for Detection of Metal Ions

Cholinesterase from the Liver of Diodon hystrix for Detection of Metal Ions

The Effect of Temperature Factor on the Detection of Nitrate Based on Planar Electromagnetic Sensor and Independent Component Analysis

Whole-cell aquatic biosensors

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