Rapid characterization of TCM Qianjinteng by UPLC-QTOF-MS and its application in the evaluation of three species of Stephania

Xiao, Jiangjian; Song, Nan; Tong, L.; Pan, Yingni; Song, Junyu; Chen, Gang; Sun, Lixin; Li, Ning

doi:10.1016/j.jpba.2018.04.044

Cited by 37 publications

(26 citation statements)

References 27 publications

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“…To date, numerous analytical methods have been developed, which may facilitate structural determination of the alkaloids in traditional herbal medicines, and include thin‐layer chromatography (TLC), high‐performance liquid chromatography (HPLC), LC‐electrospray ionisation (ESI) tandem mass spectrometry (MS/MS), capillary electrophoresis (CE)/ion spray mass spectrometry (MS), gas chromatography mass spectrometry (GC‐MS), and nuclear magnetic resonance (NMR). Among these analytical methods, the HPLC method has been proposed as an incisive tool for the identification of alkaloids and quality control of plant material …”

Section: Introductionmentioning

confidence: 99%

“…Among these analytical methods, the HPLC method has been proposed as an incisive tool for the identification of alkaloids and quality control of plant material. [6][7][8][9] Our investigation is focused on the search of extracts or molecule with specific activity against insects. The present article deals with the insecticide effects associated at the components of the roots of Talguenea quinquinervia.…”

Section: Introductionmentioning

confidence: 99%

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Identification of structurally diverse alkaloids in Talguenea quinquinervia (gill. Et hook) by liquid chromatography/electrospray ionisation tandem mass spectroscopy and insecticidal activity

Quiroz-Carreño

Cespedes‐Acuña

Seigler

et al. 2019

Phytochemical Analysis

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Introduction Talguenea quinquinervia has been used in folk medicine and to dye wool, and the main constituents are alkaloids and triterpenes. Identification of these type of compounds in this specie is a necessary step to understand the biological properties. Objective To evaluate the relationship between the chemical composition of root from T. quinquinervia and its insecticidal properties using liquid chromatography electrospray ionisation tandem mass spectrometry (LC‐ESI‐MS/MS). Methods Alkaloids were extracted using Soxhlet extraction with methanol. Total extract was partitioned at pH 2 and 12 to enrich alkaloid constituents and to remove interferences. The separation of alkaloids in the Talguenea extract was performed on a C18 column using gradient elution and their tandem mass spectra were obtained by quadrupole time‐of‐flight tandem mass spectrometry (QTOF‐MS/MS) to perform accurate mass measurements of fragment ions for the alkaloid constituents. Results Several types of alkaloids were separated and identified by LC‐ESI‐MS/MS. The structural assignment of individual alkaloids was performed based on convergence of MS/MS spectral data, pH partitioning behaviour, LC retention behaviour, and accurate mass measurements. The pH partition of the extract provided structural information about unknown alkaloids extracted from T. quinquinervia. A total of 20 compounds were identified and tentatively characterised, and of these 15 alkaloids were reported for the first time in the investigated T. quinquinervia. Conclusion The chemical profiling of alkaloids in T. quinquinervia with different origins was performed for the first time and provided diagnostic ions for diverse alkaloids in T. quinquinervia. Insecticidal activity observed can be explain by the presence of alkaloid and pentacyclic triterpenes on the fractions assayed.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of structurally diverse alkaloids in Talguenea quinquinervia (gill. Et hook) by liquid chromatography/electrospray ionisation tandem mass spectroscopy and insecticidal activity

Quiroz-Carreño

Cespedes‐Acuña

Seigler

et al. 2019

Phytochemical Analysis

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“…Eight monobenzyltetrahydroisoquinolines have been reported to be present in S. tetrandra ( Fig. 2): (1) Nmethylcoclaurine (Xiao et al 2018); (2) juziphine (Xiao et al 2018); (3) coclaurine (Xiao et al 2018;Sim et al 2015); (4) protosinomenine (Xiao et al 2018); (5) reticuline (Xiao et al 2018); (6) coclaurine-7-O-b-D-glucopyranoside (Sim et al 2015); (7) oblongine (Sim et al 2015) and (8) oblongine chloride (Wino et al 1988). Of these, N-methylcoclaurine, juziphine, coclaurine, protosinomenine, reticuline, coclaurine-7-O-b-D -glucopyranoside and oblongine have been tentatively identified using liquid Terandrine R and A Sim et al (2013Sim et al ( , 2015, Wino et al (1988), Tsutsumi et al (2003), Ogino et al (1998), Li et al (2009a, b), Si et al (1992), Si and Zhao (1991), Deng et al (1990)…”

Section: Monobenzyltetrahydroisoquinolinesmentioning

confidence: 99%

“…Bisbenzyltetrahydroisoquinolines are the major active constituents in S. tetrandra. To date, 30 bisbenzyltetrahydroisoquinolines have been identified as constituents (Xiao et al 2018;Sim et al 2013Sim et al , 2015Wino et al 1988;Tsutsumi et al 2003;Ogino et al 1998;Li et al 2009a;Si et al 1992;Si and Zhao 1991;Deng et al 1990; Tian 2014) ( Fig. 3).…”

Section: Bisbenzyltetrahydroisoquinolinesmentioning

confidence: 99%

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A critical review: traditional uses, phytochemistry, pharmacology and toxicology of Stephania tetrandra S. Moore (Fen Fang Ji)

2020

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Stephania tetrandra S. Moore (S. tetrandra) is distributed widely in tropical and subtropical regions of Asia and Africa. The root of this plant is known in Chinese as ''Fen Fang Ji''. It is commonly used in traditional Chinese medicine to treat arthralgia caused by rheumatism, wet beriberi, dysuria, eczema and inflamed sores. Although promising reports have been published on the various chemical constituents and activities of S. tetrandra, no review comprehensively summarizes its traditional uses, phytochemistry, pharmacology and toxicology. Therefore, the review aims to provide a critical and comprehensive evaluation of the traditional use, phytochemistry, pharmacological properties, pharmacokinetics and toxicology of S. tetrandra in China, and meaningful guidelines for future investigations.

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Comparison of chemical constituents of Bupleurum marginatum var. stenophyllum and Bupleurum chinense DC. using UHPLC–Q‐TOF–MS based on a metabonomics approach

Xia

Liu

Tong

et al. 2021

Biomedical Chromatography

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The overall chemical composition of Bupleurum marginatum var. stenophyllum and Bupleurum chinense DC. was compared in this study. Metabolites were identified using ultra‐high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry. Multivariate statistical analysis techniques such as principal component analysis were used to conduct metabonomics analysis and study the correlation between different components. Principal component analysis results showed a clear distinction among medicinal materials of different origins and divided them into different categories, consistent with the results of hierarchical cluster analysis. Both partial least squares discriminant analysis (PLS‐DA) and orthogonal partial least squares discriminant analysis (OPLS‐DA) showed that the two materials could be distinguished clearly. Using PLS‐DA and OPLS‐DA combined with the S‐plot and a variable importance in the projection (VIP) score >1, 24 differential metabolites were screened and identified; all of the metabolites were triterpenoid saponins. In addition, SPSS 25.0 and Metabo Analyst 4.0 were used to analyze significant differences in the relative contents of different compounds in the two materials. This study has successfully provided not only a new direction for research based on the chemical substances identified and the quality evaluation of Bupleuri Radix but also a better theoretical basis for the expansion of medicinal sources and their clinical application.

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Rapid characterization of TCM Qianjinteng by UPLC-QTOF-MS and its application in the evaluation of three species of Stephania

Cited by 37 publications

References 27 publications

Identification of structurally diverse alkaloids in Talguenea quinquinervia (gill. Et hook) by liquid chromatography/electrospray ionisation tandem mass spectroscopy and insecticidal activity

Identification of structurally diverse alkaloids in Talguenea quinquinervia (gill. Et hook) by liquid chromatography/electrospray ionisation tandem mass spectroscopy and insecticidal activity

A critical review: traditional uses, phytochemistry, pharmacology and toxicology of Stephania tetrandra S. Moore (Fen Fang Ji)

Comparison of chemical constituents of Bupleurum marginatum var. stenophyllum and Bupleurum chinense DC. using UHPLC–Q‐TOF–MS based on a metabonomics approach

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