Rapid characterization of feline leukemia virus infective stages by a novel nested recombinase polymerase amplification (RPA) and reverse transcriptase-RPA

Lacharoje, Sitthichok; Techangamsuwan, Somporn; Chaichanawongsaroj, Nuntaree

doi:10.1038/s41598-021-01585-9

Cited by 11 publications

(14 citation statements)

References 44 publications

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“…They reported nested recombinase polymerase amplification (nRPA) and RT-RPA for the detection of FeLV DNA provirus and FeLV RNA, respectively. 14 Recently, Ghosh et al also reported two recombinase-based assays; RT-RPA and reverse transcription recombinase-aided amplification (RT-RAA) in the detection of SARS-CoV-2. Their findings revealed an equally promising diagnostic accuracy of the two methods, with a sensitivity of 76.32% (95% CI: 65.18-85.32%) by using RdRP and ORF1ab genes for RT-RPA and RT-RAA, respectively.…”

Section: Discussionmentioning

confidence: 99%

Lateral Flow Recombinase Polymerase Amplification Assays for the Detection of Human Plasmodium Species

Lai

Hamid

Jelip

et al. 2023

The American Journal of Tropical Medicine and Hygiene

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This study highlights the development of two lateral flow recombinase polymerase amplification assays for the diagnosis of human malaria. The lateral flow cassettes contained test lines that captured biotin-, 6-carboxyfluorescein, digoxigenin-, cyanine 5-, and dinitrophenyl-labeled amplicons. The overall process can be completed in 30 minutes. Recombinase polymerase amplification coupled with lateral flow had a detection limit of 1 copy/µL for Plasmodium knowlesi, Plasmodium vivax, and Plasmodium falciparum. No cross-reactivity was observed among nonhuman malaria parasites such as Plasmodium coatneyi, Plasmodium cynomolgi, Plasmodium brasilanium, Plasmodium inui, Plasmodium fragile, Toxoplasma gondii, Sarcocystis spp., Brugia spp., and 20 healthy donors. It is rapid, highly sensitive, robust, and easy to use. The result can be read without the need for special equipment and thus has the potential to serve as an effective alternative to polymerase chain reaction methods for the diagnosis of malaria.

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Section: Discussionmentioning

confidence: 99%

Lateral Flow Recombinase Polymerase Amplification Assays for the Detection of Human Plasmodium Species

Lai

Hamid

Jelip

et al. 2023

The American Journal of Tropical Medicine and Hygiene

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“…In our study, 64% of the cats diagnosed with mediastinal lymphoma were FeLV positive by using a commercial test kit (immunochromatography assay), which detects the p27 antigen of the virus. The most recent study using nested recombinase polymerase amplification (nRPA) to detect exogenous FeLV DNA provirus and RT-PCR to detect FeLV RNA, showed that the FeLV detection rate in the regression phase was increased up to 45.8% compared to the rapid immunochromatographic assay ( 26 ). The number of FeLV-positive lymphomatous effusion in our study might have been higher if the molecular detection of the virus was used.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic utility of LDH measurement for determining the etiology of modified transudate pleural effusion in cats

Hla

Hunprasit²,

Siripoonsup³

et al. 2022

Front. Vet. Sci.

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Fluid analysis is an initial approach for determining the underlying causes of body cavity effusions. Modified transudate is commonly diagnosed in pleural effusion in cats, however, it provides limited diagnostic information. Aims of this study were to investigate common etiologies causing different pleural fluid types and to evaluate the usefulness of lactate dehydrogenase (LDH) for differentiating the etiology in modified transudates in cats. Pleural effusion samples from 122 cats were analyzed and classified into three types: transudate, modified transudate, and exudate. Causes of pleural effusion were classified into four conditions: cardiac disease, neoplasia, feline infectious peritonitis (FIP), and pyothorax. The relationship of underlying etiology and fluid types was described. The LDH levels in pleural fluid and plasma were compared between the causes in the samples classified as modified transudate. The fluid analysis of pleural effusion showed that modified transudate was the most common fluid type (44.2%). Neoplasia was predominantly diagnosed (38.5%) as the etiology of pleural effusion. There was no significant correlation between pleural fluid and plasma LDH level in any type of pleural fluid, suggesting that pleural fluid LDH does not appear to be affected by plasma LDH. The occurrence of modified transudate was not associated to its etiologies, however, the LDH level in modified transudates showed significant differences between etiologic groups. The LDH level in modified transudate was excellent in separating cardiac from non-cardiac diseases with a cut-off value of <535 U/L and separating FIP from non-FIP diseases with a cut-off value of >641 U/L. Based on the current findings, pleural fluid LDH can be a useful adjunctive marker for differentiating some causes of modified transudate pleural effusion and should be added in the routine diagnostic work-up of feline patients with pleural effusions.

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“…Podczas transmisji poziomej, zakażenie przenosi się przez bezpośredni kontakt, zwykle przez styczność ze śliną, np. przez jedzenie i picie ze wspólnej miski lub pokąsanie przez chorego osobnika (16). Zwierzęta mogą również zakazić się poprzez kontakt z wydzielinami i wydalinami, takimi jak: kał, mleko, mocz, płyny ustrojowe.…”

Section: Epidemiologia I Drogi Transmisjiunclassified

“…Zwierzęta mogą również zakazić się poprzez kontakt z wydzielinami i wydalinami, takimi jak: kał, mleko, mocz, płyny ustrojowe. Możliwa jest także transmisja wirusa w sposób jatrogenny podczas transfuzji krwi (16,17,20). FeLV jest przenoszony również przez ektopasożyty, takie jak pchły (30).…”

Section: Epidemiologia I Drogi Transmisjiunclassified

“…U starszych kotów, uzyskujących wraz z wiekiem większą odporność na zakażenie FeLV, objawy kliniczne są niespecyficzne (11). Najczęściej wymienia się: apatię, spadek masy ciała, powiększenie węzłów chłonnych, bladość widocznych błon śluzowych oraz gorączkę (16). Często wirus zostaje wykryty przypadkowo w czasie rutynowej diagnostyki zwierząt, u których najczęściej stwierdzano objawy związane z występowaniem zakażania wirusem FIP (90% kotów) oraz FIV (3,5%) ( 16), zakażenia mykoplazmą, zapalenia jamy ustnej (15%), niedokrwistości (11%) oraz chłoniaka (6%) (11).…”

Section: Patogenezaunclassified

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Feline leukemia as a still relevant problem in domestic and wild animals – the current state of knowledge

ZDZIENNICKA¹,

DOBKO²,

DRZEWIECKA³

et al. 2023

Medycyna Weterynaryjna

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The aim of this review is to present information about leukemia caused by feline leukemia virus (FeLV) infection in felids. Despite widespread vaccinations, leukemia is still one of the most common diseases in cats. FeLV infection occurs by direct contact between animals, especially through contact with saliva. Infection with this highly contagious virus is characterized by immunosuppression and non-specific signs, such as cytopenia or tumorigenesis. The virus is transmitted horizontally through close contact between cats and vertically from infected queens to their kittens. Persistently infected cats are the reservoir of the virus. Rapid ELISA tests and immunochromatography play a major role in diagnosis, but RT-PCR is the most reliable diagnostic method. There is no specific therapy against FeLV, so treatment is mainly symptomatic. Research on the use of antiviral drugs, i.e. tenofovir, raltegravir, and fozyvudine, is underway. Proper prophylaxis, including regular vaccination of animals, is important to minimize the risk and consequences of infection

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Rapid characterization of feline leukemia virus infective stages by a novel nested recombinase polymerase amplification (RPA) and reverse transcriptase-RPA

Cited by 11 publications

References 44 publications

Lateral Flow Recombinase Polymerase Amplification Assays for the Detection of Human Plasmodium Species

Lateral Flow Recombinase Polymerase Amplification Assays for the Detection of Human Plasmodium Species

Diagnostic utility of LDH measurement for determining the etiology of modified transudate pleural effusion in cats

Feline leukemia as a still relevant problem in domestic and wild animals – the current state of knowledge

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