Rapid cation-exchange chromatography of hemoglobins and other proteins

Burke, David; Duncan, J.Keith; Siebert, Chris; Ott, Gary

doi:10.1016/0021-9673(86)80104-2

Cited by 46 publications

(3 citation statements)

References 15 publications

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“…Burke et al 32 also used micropellicular ion-exchange chromatography to separate hemoglobin variants, although the particular column used by these workers is no longer commercially available. At moderate flow rates, the resolution achieved by Burke et al was somewhat less than that shown in Figure 2B.…”

Section: Resultsmentioning

confidence: 99%

Chromatofocusing Using Micropellicular Column Packings with Computer-Aided Design of the Elution Buffer Composition

Kang

Frey

2002

Anal. Chem.

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Micropellicular, anion-exchange column packings are used in chromatofocusing to demonstrate the resolution and speed achieved when proteins are separated under these conditions. Linear or concave pH gradients are produced with simple mixtures containing four or fewer individual buffering species instead of the more commonly used polyampholyte buffers. Computer-aided design methods are demonstrated for selecting the composition of the elution buffer to produce a pH gradient of a desired shape. The method is applied to high-resolution, analytical- and preparative-scale separations involving horse myoglobin, human hemoglobin variants, and bovine carbonic anhydrase. A useful selection of buffering species is described capable of producing pH gradients of a variety of shapes in the range between pH 9.5 and 5.5.

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Section: Resultsmentioning

confidence: 99%

Chromatofocusing Using Micropellicular Column Packings with Computer-Aided Design of the Elution Buffer Composition

Kang

Frey

2002

Anal. Chem.

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“…A major advantage of the pellicular configuration is that there are no significant intraparticular diffusion resistances [9], which is particularly useful for the rapid analysis of biopolymers with high efficiency and resolution [10]. At the same time, the lack of internal pore structure offers certain other advantages such as good recovery of mass and biological activity in protein chromatography [11].…”

Section: Properties Of Poly(styrene-divinylbenzene) Particlesmentioning

confidence: 99%

Poly(Styrene‐Divinylbenzene) Based Media for Liquid Chromatography

Huck

Bonn

2005

Chem Eng & Technol

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The latest developments and in particular important synthetic aspects for the preparation of modern poly(styrene-divinylbenzene) (PS-DVB) based liquid chromatography (LC) supports are reviewed. In this context, the chemistry of particular porous and nonporous, functionalized, monolithic, coated silica and more specialized mixed organic PS-DVB media is covered. Special consideration is given to modern approaches such as micro-(l)-HPLC and coating techniques and their most important applications. Synthetic particularities relevant to the corresponding applications are outlined.

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“…IEC separation of ionic forme of the estrogen receptors from human breast cancer tiesue on AX-300[64]. Cytosol WLIE prepared from human breast cancer tissue and incubated in the presence (0) or absence ( l ) of 500-fold excess diethylstilbeatrol (DES).…”

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confidence: 99%

Chromatography of complex protein mixtures

Regnier

1987

Journal of Chromatography B: Biomedical Sciences and Applicatio

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Rapid cation-exchange chromatography of hemoglobins and other proteins

Cited by 46 publications

References 15 publications

Chromatofocusing Using Micropellicular Column Packings with Computer-Aided Design of the Elution Buffer Composition

Chromatofocusing Using Micropellicular Column Packings with Computer-Aided Design of the Elution Buffer Composition

Poly(Styrene‐Divinylbenzene) Based Media for Liquid Chromatography

Chromatography of complex protein mixtures

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