Rapid Cancer Detection by Topically Spraying a γ-Glutamyltranspeptidase–Activated Fluorescent Probe

Urano, Yasuteru; Sakabe, Masayo; Kosaka, Nobuyuki; Ogawa, Mikako; Mitsunaga, Makoto; Asanuma, Daisuke; Kamiya, Mako; Young, Matthew R.; Nagano, Tetsuo; Choyke, Peter L.; Kobayashi, Hisataka

doi:10.1126/scitranslmed.3002823

Cited by 409 publications

(437 citation statements)

References 31 publications

Supporting

Mentioning

432

Contrasting

Unclassified

Order By: Relevance

“…203 The concept of enzymatically activatable tracers has already been successfully applied in the field of oncology and the concept will -in our view-also be very well applicable in the field of infection imaging. 204 Category 4 includes the application of bacteriophages and targeting quorum sensing. These approaches have a high potential through their selectivity for individual classes of bacteria.…”

Section: Discussionmentioning

confidence: 99%

Development and Prospects of Dedicated Tracers for the Molecular Imaging of Bacterial Infections

et al. 2013

View full text Add to dashboard Cite

Abstract:Bacterial infections have always been, and still are, a major global healthcare problem. For accurate treatment it is of upmost importance that the location(s), severity, type of bacteria, and therapeutic response can be accurately staged. Similar to the recent successes in oncology, tracers specific for molecular imaging of the disease may help advance the patient management. Chemical design and bacterial targeting mechanisms are the basis for the specificity of such tracers. The aim of this review is to provide a comprehensive overview of the molecular imaging tracers developed for optical and nuclear identification of bacteria and bacterial infections. Hereby we envision that such tracers can be used to diagnose infections and aid their clinical management. From these compounds we have set-out to identify promising targeting mechanisms and select the most promising candidates for further development.

show abstract

Section: Discussionmentioning

confidence: 99%

Development and Prospects of Dedicated Tracers for the Molecular Imaging of Bacterial Infections

et al. 2013

View full text Add to dashboard Cite

show abstract

“…The present report further emphasizes the importance of resecting the TME in FGS. FGS is now emerging in the clinic (29)(30)(31) and should become the standard paradigm in the near future…”

Section: Ex Vivo Color-coded Imaging Of Cancer-cell and Host Stromalcmentioning

confidence: 99%

Color-coded Imaging Enables Fluorescence-guided Surgery to Resect the Tumor Along with the Tumor Microenvironment in a Syngeneic Mouse Model of EL-4 Lymphoma

Hasegawa

Suetsugu

Nakamura

et al. 2016

View full text Add to dashboard Cite

Abstract. Background Fluorescence-guided surgery (FGS) of cancer is an emerging technology. Our laboratory has pioneered mouse models using fluorescent proteins, as well as fluorescent antibodies, to label tumors for FGS (1-11).To achieve complete tumor resection, it is necessary to understand the relationship between cancer cells and stromal cells in the tumor microenvironment (TME) (9). We previously reported imaging of the TME during tumor progression and metastasis by color-coding cancer and stromal cells (12-27).We have subsequently used genetic reporters to color code cancer and stroma cells in a patient-derived orthotopic xenograft (PDOX) model. Cancer cells were labeled with a telomerasedependent green fluorescent protein (GFP)-containing adenovirus (OBP-401) in a pancreatic cancer PDOX. The PDOX was labeled in the stroma by red fluorescent protein (RFP) during growth in transgenic mice that express RFP (9).In the present report, we developed a color-coded syngeneic model for FGS using a mouse lymphoma cell line (EL-4), expressing RFP, and transgenic C57/B6 mice ubiquitously expressing GFP under the control of the chicken β-actin promoter and cytomegalovirus enhancer. Using the portable, hand-held Dino-Lite fluorescence microscope, the EL-4-RFP tumor in the GFP mouse was color-coded imaged in order to visualize the tumor and TME and resected by FGS. Confocal microscopy of the resected tumor demonstrated that both tumor and the TME were completely resected by color-coded FGS. In the resected RFP-expressing tumor, various types of GFPexpressing stromal cells were observed intermingling with the cancer cells in the TME. 4443

show abstract

“…1 The researchers will next validate the method in surgical tissue from ovarian cancer patients before submitting an IND.…”

Section: By Joanne Kotz Senior Editormentioning

confidence: 99%

Eau de fluorescence

Kotz¹

2012

Science-Business eXchange

View full text Add to dashboard Cite

Rapid Cancer Detection by Topically Spraying a γ-Glutamyltranspeptidase–Activated Fluorescent Probe

Cited by 409 publications

References 31 publications

Development and Prospects of Dedicated Tracers for the Molecular Imaging of Bacterial Infections

Development and Prospects of Dedicated Tracers for the Molecular Imaging of Bacterial Infections

Color-coded Imaging Enables Fluorescence-guided Surgery to Resect the Tumor Along with the Tumor Microenvironment in a Syngeneic Mouse Model of EL-4 Lymphoma

Eau de fluorescence

Contact Info

Product

Resources

About