Rapid calcification on solution blending of homogenous PHBV/collagen composite

Abstract

Developing of a surface hybrid calcium phosphate-degradable polymer materials is always attractive since it could have high bone bonding ability and proper mechanical properties as natural bone substitute. In this article, a solution blending method was used to make the poly-[(3-hydroxybutyrate)-co-(3-hydroxyvalerate)] (PHBV)/collagen composite with high surface calcium apatite deposition capability. Before the blending, the pseudo ternary phase diagram of CH 3 COOH-CHCl 3 / PHBV-H 2 O/collagen at 20 C was plotted. Three compositions selected from the homogenous zone in the diagram were chosen to fabricate the PHBV/collagen composites. Water contact angle, water absorbance ratio, and FTIR spectra showed the collagen contents in the final composites increased with its original blending ratio. The composite with the highest collagen content was chosen for the biomineralization experiment in simulated calcium solution (SCS). Scanning electronic microscopy showed the morphology of the deposition changing from porous structure to plate structure. The FTIR spectra displayed the precipitate was calcium phosphate containing collagen content. The X-ray Diffractometer spectra displayed that the precipitate has the similar characteristic peaks as calcium apatite and the crystalline increased with the biomineralization time. The concentration of calcium and hydrogenphosphate ions in the SCS monitored in the first 4 h decreased to 29.5 and 1.8 ppm, respectively. The Ca/P of the precipitate stabilized at 1.3 in the first 4 h. The FTIR spectra showed that the amide absorption band shifted to low wavelength, which was caused by the electrostatic force between the calcium ions and the amide bond p-p conjugation system.

pH‐responsive variation of biomineralization via collagen self‐assembly and the simultaneous formation of apatite minerals

“…In terms of the design and synthesis of an ideal biomimetic material, it is necessary to mimic both the composition and microstructure of natural bone . Collagen biomineralization is an innovative method to fabricate mineralized collagen fibrils with a hierarchically ordered structure that consists of mineral and organic phases . Understanding the strategies of collagen biomineralization is tremendously significant for the development of biomimetic materials …”

Blocking of matrix metalloproteinases-13 responsive peptide in poly(urethane urea) for potential cartilage tissue engineering applications

“…13,14 However, the blended materials exhibit the poor mechanical strength and unpredictable degradation rate of materials due to their heterogeneous structures caused by the weak intermolecular interactions between hydrophilic ECMs and hydrophobic polymers. 15 Blocking protease sensitive molecular structures in polymer chains forms homogenous material structures with improved properties. For example, Benhardt et al developed a poly(urethane urea) (PUU) by introducing the peptide segment GPQGIWGQGK into ether-based polyols as the primary MMP-2 responsive labile site for ligament tissue engineering application.…”

“…13,14 However, the blended materials exhibit the poor mechanical strength and unpredictable degradation rate of materials due to their heterogeneous structures caused by the weak intermolecular interactions between hydrophilic ECMs and hydrophobic polymers. 15…”