Rapid blood group genotyping by allelic discriminative real‐time PCR in multiply‐transfused patients

Rodrigues, Evandra Strazza; Macedo, Mayra Dorigan de; Melo, Fernanda Ursoli de; Rocha–Junior, Maurício Cristiano; Wagatsuma, Virginia Mara De Deus; Toledo, Ricardo Acayaba de; Ubiali, Eugênia Maria Amorim; Brunetta, Denise Menezes; Covas, Dimas Tadeu; Kashima, Simone

doi:10.1111/tme.12186

Cited by 4 publications

(1 citation statement)

References 10 publications

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“…E. S. Rodrigues и соавт. [41] предложили для быстрого и надежного генотипирования групп крови систем резус, MNS, Кидд и Даффи у пациентов с многократными гемотрансфузиями оптимизированную распознающую аллели в реальном времени ПЦР. Применение серологических методов определения групповых факторов крови имеет ограничения, например, у недавно перенесших трансфузии больных (химеризм) и у больных с положительным прямым антиглобулиновым тестом.…”

Section: обзор литературыunclassified

GENOTYPING OF AB0 AND Rh SYSTEMS BLOOD GROUPS IN PATIENTS AFTER MULTIPLE HEMOTRANSFUSIONS

et al. 2017

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Фундаментальные исследования в практической медицине на современном этапе Введение Пациенты с неэффективным гемопоэзом и анемией нуждаются в частых трансфузиях эритроцитов. В крови пациентов, перенесших трансфузии в течение 4 предшествующих месяцев, циркулируют как минимум 2 популяции эритроцитов-собственные

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Section: обзор литературыunclassified

GENOTYPING OF AB0 AND Rh SYSTEMS BLOOD GROUPS IN PATIENTS AFTER MULTIPLE HEMOTRANSFUSIONS

et al. 2017

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Frequency and characterization of RHD variant alleles in a population of blood donors from southeastern Brazil: Comparison with other populations

Rodrigues

Romagnoli

Santos

et al. 2021

Transfusion and Apheresis Science

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Accuracy of RHC/c genotyping in Chinese Han population

Zhou,

Liu,

Shao

et al. 2024

Transfusion Medicine

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Background and ObjectivesThe RHCE gene plays an important role in the complex and polymorphic Rh blood group system. RHCE genotyping holds significant clinical and transfusion‐related implications. The objective of this study was to evaluate the accuracy of RHC/c genotyping in the Chinese Han population.Materials and MethodsBlood samples were obtained from 653 Chinese Han blood donors. The serological RhD and RhCcEe types were determined using monoclonal antibodies. Subsequently, multiplex real‐time polymerase chain reaction (PCR) analysis was performed for RHC and RHc genotyping. Additionally, exon 2 of RHCE and exon 1 of RHD were sequenced.ResultsThe analysis in this study found 443 RhD‐positive donors and 210 RhD‐negative donors. Among the 653 total donors, discrepancies between the RHC genotyping results and the serological results were found in 37 individuals. Specifically, 6 false‐positive RhC results in RhD‐positive donors and 28 false‐positive RhC results in RhD‐negative donors were identified based on c.48C in RHCE exon 1. Additionally, 3 false‐negative RhC results were observed in the RhD‐positive donors due to a 109 bp insertion in RHCE intron 2. RHc typing demonstrated complete consistency between the real‐time PCR and the serological results.ConclusionIn the Chinese Han population, RHC genotyping was reliable when consistent results were achieved by both c.48C‐based and 109 bp insertion‐based genotyping. Moreover, RHc genotyping based on c.203A and c.307C polymorphic loci demonstrated dependable performance.

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Rapid blood group genotyping by allelic discriminative real‐time PCR in multiply‐transfused patients

Cited by 4 publications

References 10 publications

GENOTYPING OF AB0 AND Rh SYSTEMS BLOOD GROUPS IN PATIENTS AFTER MULTIPLE HEMOTRANSFUSIONS

GENOTYPING OF AB0 AND Rh SYSTEMS BLOOD GROUPS IN PATIENTS AFTER MULTIPLE HEMOTRANSFUSIONS

Frequency and characterization of RHD variant alleles in a population of blood donors from southeastern Brazil: Comparison with other populations

Accuracy of RHC/c genotyping in Chinese Han population

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