2015
DOI: 10.1111/tme.12186
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Rapid blood group genotyping by allelic discriminative real‐time PCR in multiply‐transfused patients

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Cited by 4 publications
(3 citation statements)
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“…In this study, we focused on the RHCE gene, which is one of the most clinically relevant blood group systems in transfusion as it displays high immunogenicity and great diversity [2][3][4][5][6][7][8][9]. In routine immunohematology, the drawbacks of serological techniques are compensated for by genotyping methods [12][13][14][15][16][17][18]. In some situations, however, samples may show ambiguities or discrepancies that require custom analyses.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…In this study, we focused on the RHCE gene, which is one of the most clinically relevant blood group systems in transfusion as it displays high immunogenicity and great diversity [2][3][4][5][6][7][8][9]. In routine immunohematology, the drawbacks of serological techniques are compensated for by genotyping methods [12][13][14][15][16][17][18]. In some situations, however, samples may show ambiguities or discrepancies that require custom analyses.…”
Section: Discussionmentioning
confidence: 99%
“…In the context of routine immunohematology, serological techniques enable us to characterize most of the Rh phenotypes, and within the last decade, molecular biology solutions have helped compensate for the main limitations of serological techniques [ 12 , 13 , 14 , 15 , 16 , 17 , 18 ]. Still, in some situations, ambiguities or discrepancies cannot be resolved, especially for RhCE, and uncertainties remain regarding the transfusion instructions; in this context, the French Blood Center, whose main mission is to ensure France’s self-sufficiency in blood products under optimal safety and quality conditions, is continuously setting up molecular typing strategies in line with technological developments [ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ].…”
Section: Introductionmentioning
confidence: 99%
“…E. S. Rodrigues и соавт. [41] предложили для быстрого и надежного генотипирования групп крови систем резус, MNS, Кидд и Даффи у пациентов с многократными гемотрансфузиями оптимизированную распознающую аллели в реальном времени ПЦР. Применение серологических методов определения групповых факторов крови имеет ограничения, например, у недавно перенесших трансфузии больных (химеризм) и у больных с положительным прямым антиглобулиновым тестом.…”
Section: обзор литературыunclassified