Rapid biodegradation of polycyclic aromatic hydrocarbons (PAHs) using effective Cronobacter sakazakii MM045 (KT933253)

Umar, Zubairu Darma; Aziz, Nor Azwady Abd; Zulkifli, Syaizwan Zahmir; Mustafa, Muskhazli

doi:10.1016/j.mex.2017.02.003

Cited by 38 publications

(17 citation statements)

References 32 publications

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“…The occurrence of 1,2-naphthalenediol in the naphthalene-incubated culture reconfirmed that RHD-3 is a naphthalene dioxygenase. In addition, 3,4-dihydroxyphenanthrene and 2-carboxy-1- naphthol were detected in the phenanthrene-and pyrene-grown cultures, which is consistent with intermediates produced by Sphingomonas, Cronobacter, and Enterobacter (52,53). 3,4-Dihydroxyphenanthrene was generated as an intermediate in phenanthrene degradation, which reconfirmed that RHD-2 was active in phenanthrene hydroxylation.…”

supporting

confidence: 72%

Polycyclic Aromatic Hydrocarbon (PAH) Degradation Pathways of the Obligate Marine PAH Degrader Cycloclasticus sp. Strain P1

Wang

Shao

2018

Appl Environ Microbiol

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Bacteria play an important role in the removal of polycyclic aromatic hydrocarbons (PAHs) from polluted environments. In marine environments, is one of the most prevalent PAH-degrading bacterial genera. However, little is known regarding the degradation mechanisms for multiple PAHs by sp. strain P1 was isolated from deep-sea sediments and is known to degrade naphthalene, phenanthrene, pyrene, and other aromatic hydrocarbons. Here, six ring-hydroxylating dioxygenases (RHDs) were identified in the complete genome of sp. P1 and were confirmed to be involved in PAH degradation by enzymatic assays. Further, five gene clusters in its genome were identified to be responsible for PAH degradation. Degradation pathways for naphthalene, phenanthrene, and pyrene were elucidated in sp. P1 based on genomic and transcriptomic analysis and characterization of an interconnected metabolic network. The metabolic pathway overlaps in many steps in the degradation of pyrene, phenanthrene, and naphthalene, which were validated by the detection of metabolic intermediates in cultures. This study describes a pyrene degradation pathway for Moreover, the study represents the integration of a PAH metabolic network that comprises pyrene, phenanthrene, and naphthalene degradation pathways. Taken together, these results provide a comprehensive investigation of PAH metabolism in PAHs are ubiquitous in the environment and are carcinogenic compounds and tend to accumulate in food chains due to their low bioavailability and poor biodegradability. is an obligate marine PAH degrader and is widespread in marine environments, while the PAH degradation pathways remain unclear. In this report, the degradation pathways for naphthalene, phenanthrene, and pyrene were revealed, and an integrated PAH metabolic network covering pyrene, phenanthrene, and naphthalene was constructed in This overlapping network provides streamlined processing of PAHs to intermediates and ultimately to complete mineralization. Furthermore, these results provide an additional context for the prevalence of in oil-polluted marine environments and pelagic settings. In conclusion, these analyses provide a useful framework for understanding the cellular processes involved in PAH metabolism in an ecologically important marine bacterium.

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confidence: 72%

Polycyclic Aromatic Hydrocarbon (PAH) Degradation Pathways of the Obligate Marine PAH Degrader Cycloclasticus sp. Strain P1

Wang

Shao

2018

Appl Environ Microbiol

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“…The previously reported PAHs degradation confirmed that C. sakazakii MM045 and Enterobacter sp. MM087 generate relevant degradation intermediates during their response (Umar et al, 2017;in press). Such effective degradation response could be the reason for excellent catabolic response of the formulated consortium.…”

Section: Discussionmentioning

confidence: 99%

“…The previously identified C. sakazakii MM045 and Enterobacter sp. MM087 were used for the preparation of bacteria inoculums whose optimum degradation responses were determined (Umar et al, 2017;in press;Darma et al, 2016). Bacteria resting cells of each individual bacterium strain was prepared separately as the pure isolates were initially grown at 35 0 C for 24 hours under 160 rpm (Sogani et al, 2012).…”

Section: Inoculums Preparationmentioning

confidence: 99%

“…Considering the importance of applying mixed bacterial consortium during PAHs biodegradation in a complex system, the previously identified effective degrading isolates C. sakazakii MM045 and Enterobacter sp. MM087 were used in this study (Umar et al, 2017;in press;Darma et al, 2016). These bacteria were used for the formulation of more proactive PAHs degrading consortium whose main objective was to assess the resistance of the consortium to hazardous heavy metals during PAHs biodegradation.…”

Section: Introductionmentioning

confidence: 99%

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Efficiency of Polycyclic Aromatic Hydrocarbons (PAHs) Degrading Consortium in Resisting Heavy Metals During PAHs Degradation

et al. 2018

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Polycyclic aromatic hydrocarbons (PAHs) comprised of many dangerous organic pollutants which affect human cell. The choice of phenanthrene and pyrene as model substrates was based on their classification among the most hazardous PAHs group by the US EPA where they belonged to low and high molecular weights PAHs respectively. Biodegradation of these PAHs is the best strategy that completely removes such pollutants in an environmentally friendly manner. However, the bacteria involved are challenged degradation difficulties as a result of PAHs inhibitory effects to the organisms. This research is aimed at formulating phenanthrene and pyrene degrading consortium that effectively perform best even in complex mixture with hazardous heavy metals. Different bacteria consortia were formulated using the compatibility testing and mathematical permutation approach and the best consortium selected. This selected consortium was then subjected to the degradation of both phenanthrene and pyrene separately in a combined mixture with the selected heavy metals from the inductively coupled plasma optical emission spectrophotometer (ICP-OES) analysis. Consortium composition of C. sakazakii MM045 (2%, v/v) and Enterobacter sp. MM087 (2%, v/v) were found to be much effective during phenanthrene (500 mg/L) and pyrene (250 mg/L) degradation. This consortium also resisted more than 6 mg/L each of Nickel (Ni), Cadmium (Cd), Vanadium (V) and Lead (Pb) in such complex degradation which was found to be more than the concentration in the natural habitat the consortium exists prior to isolation. Such performance makes the selected consortium to be an extremely efficient tool for the PAHs degradation application as many biodegradation agents were reported to be less effective when significant concentration of Ni, Cd, V and Pb are present.

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“…In addition, the concentration of PAHs also influenced by degradation processes by microorganisms, UV light and temperature (18)(19)(20). It is known that the temperature has a role in the evaporation and reaction of PAHs in the environment [21]. Furthermore, the evaporation process can also eliminate low molecular weight PAHs in the water surface [22][23][24].…”

Section: Distribution and Composition Of Pahsmentioning

confidence: 99%

Determination of Polycyclic Aromatic Hydrocarbons (PAHs) in the Brackish Water and Sediments of Citarum Irrigation System, Pakis Jaya, Karawang, Indonesia

et al. 2020

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Fifteen priority polycyclic aromatic hydrocarbons (PAHs) were determined in the sediments and brackish water of Citarum Irrigation System (CIS), Pakis Jaya, Karawang Regency. The samples were analyzed with liquid-liquid extraction for brackish water and Soxhlet Extraction for sediments. The final concentration of PAHs was determined with Gas Chromatography-Mass Spectrometry (GC-MS) and the origins of PAHs were identified through diagnostic ratios method. The total PAH concentrations ranged from 1782 to 2386.88 ng/L in water and from 113 to 405 ng/g dry weight (dw) in sediments. The concentration of total PAHs in sediment was lower than the SQG effects range-low (3442 ng/g). High molecular weight of PAHs dominated both in sediments and water. The origins of PAHs in the sediment and water-based on diagnostic ratios were from petrogenic and pyrogenic sources including petroleum, petroleum combustion, and combustion of organic materials. Overall, environmental conditions in the Citarum irrigation system and its river mouth was moderate level of PAHs contamination. * Corresponding authors:

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Rapid biodegradation of polycyclic aromatic hydrocarbons (PAHs) using effective Cronobacter sakazakii MM045 (KT933253)

Abstract: Graphical abstract

Cited by 38 publications

References 32 publications

Polycyclic Aromatic Hydrocarbon (PAH) Degradation Pathways of the Obligate Marine PAH Degrader Cycloclasticus sp. Strain P1

Polycyclic Aromatic Hydrocarbon (PAH) Degradation Pathways of the Obligate Marine PAH Degrader Cycloclasticus sp. Strain P1

Efficiency of Polycyclic Aromatic Hydrocarbons (PAHs) Degrading Consortium in Resisting Heavy Metals During PAHs Degradation

Determination of Polycyclic Aromatic Hydrocarbons (PAHs) in the Brackish Water and Sediments of Citarum Irrigation System, Pakis Jaya, Karawang, Indonesia

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