Rapid bacteria identification from environmental mining samples using MALDI-TOF MS analysis

Avanzi, Ingrid Regina; Gracioso, Louise Hase; Baltazar, Marcela dos Passos Galluzzi; Karolski, Bruno; Perpétuo, Elen Aquino; Nascimento, Cláudio Augusto Oller do

doi:10.1007/s11356-016-8125-8

Cited by 39 publications

(21 citation statements)

References 40 publications

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“…The isolation was performed using 1.0 g of a sediment sample, containing about 600 parts per million (ppm) of copper sulfate, in Luria-Bertani (LB) with different copper sulfate concentrations (60 to 700 mg/liter) at 28°C and 160 rpm for 1 week. After that, according to Avanzi et al ( 38 ), plates with fresh solid medium and different copper sulfate concentrations were used for the isolation of single colonies.…”

Section: Methodsmentioning

confidence: 99%

Copper mining bacteria: Converting toxic copper ions into a stable single-atom copper

et al. 2021

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The chemical synthesis of monoatomic metallic copper is unfavorable and requires inert or reductive conditions and the use of toxic reagents. Here, we report the environmental extraction and conversion of CuSO4 ions into single-atom zero-valent copper (Cu0) by a copper-resistant bacterium isolated from a copper mine in Brazil. Furthermore, the biosynthetic mechanism of Cu0 production is proposed via proteomics analysis. This microbial conversion is carried out naturally under aerobic conditions eliminating toxic solvents. One of the most advanced commercially available transmission electron microscopy systems on the market (NeoArm) was used to demonstrate the abundant intracellular synthesis of single-atom zero-valent copper by this bacterium. This finding shows that microbes in acid mine drainages can naturally extract metal ions, such as copper, and transform them into a valuable commodity.

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Section: Methodsmentioning

confidence: 99%

Copper mining bacteria: Converting toxic copper ions into a stable single-atom copper

et al. 2021

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“…Since then, over 200 papers have been published per year for bacteria identification using the mass spectrometer. Advances in mass spectrometry techniques and instrument development have resulted in improved detection of bacteria species; however, most studies utilize matrix-assisted laser desorption/ionization—time of flight mass spectrometry (MALDI-TOF) [9,10,11,12,13,14,15,16,17,18,19]. Recently, in 2013 the U.S. Food and Drug Administration (FDA) approved two matrix-assisted laser desorption-time of flight-mass spectrometry (MALDI-TOF-MS)-based platforms for bacteria identification [20].…”

Section: Introductionmentioning

confidence: 99%

Identification of Pathogenic Bacteria from Public Libraries via Proteomics Analysis

Jung

Kim

Bhatt

et al. 2019

IJERPH

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Hazardous organisms may thrive on surfaces that are often exposed to human contact, including children’s library books. In this study, swab samples were taken from 42 children’s books collected from four public libraries in Texas and California. Samples were then cultivated in brain–heart infusion (BHI) medium and then in Luria broth (LB) medium containing either ampicillin or kanamycin. All 42 samples (100%) were positive for bacterial growth in normal BHI medium. Furthermore, 35 samples (83.3%) and 20 samples (47.6%) in total were positive in LB medium containing ampicillin or kanamycin, respectively. Bacterial populations were then identified in samples using an Orbitrap Fusion™ Tribrid ™ mass spectrometer, a state-of-the-art proteomic analysis tool. Identified bacterial species grown in ampicillin included Bacillus, Acinetobacter, Pseudomonas, Staphylococcus, Enterobacter, Klebsiella, Serratia, Streptococcus, Escherichia, Salmonella, and Enterococcus. In contrast, identified bacteria grown in kanamycin included Staphylococcus, Streptococcus, Enterococcus, and Bacillus. The presences of pathogenic bacteria species were also confirmed. The results of this study warrant follow up studies to assess the potential health risks of identified pathogens. This study demonstrates the utility of proteomics in identifying environmental pathogenic bacteria for specific public health risk evaluations.

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“…Further, several mass spectrometry methods were used for the classification and identification of bacteria and other microorganisms (Sauer and Kliem 2010 ). For example, MALDI-TOF MS analysis was demonstrated to be a rapid method for bacteria identification from mining samples (Avanzi et al 2017 ; Książczyk et al 2016 ; Schröttner et al 2016 ). Although many new analytical tools were recently used for the classification and identification of bacteria, there is still urgency for the discovery of new molecular markers.…”

Section: Discussionmentioning

confidence: 99%

The heat shock protein 70 gene as a new alternative molecular marker for the taxonomic identification of Streptomyces strains

Sun

Liu

2018

AMB Expr

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With the developments in taxonomy, the classically used highly conserved 16S rRNA molecular marker has shown some disadvantages among closely related species. For further taxonomic studies of the prokaryotes, specific PCR primers were designed from two conserved regions in the amino acid sequences of the 70-kDa heat shock protein sourced from 20 different genera in actinomycetes. These were used for the amplification of the hsp70 genes in 16 Streptomyces strains. Then, we investigated the phylogenetic relationships among these Streptomyces strains and compared the tree topology based on the hsp70 gene with those based on the previously used markers (16S rRNA and gyrB). To our knowledge, this is the first use of the hsp70 gene as a molecular marker for the taxonomic identification of Streptomyces.

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Rapid bacteria identification from environmental mining samples using MALDI-TOF MS analysis

Cited by 39 publications

References 40 publications

Copper mining bacteria: Converting toxic copper ions into a stable single-atom copper

Copper mining bacteria: Converting toxic copper ions into a stable single-atom copper

Identification of Pathogenic Bacteria from Public Libraries via Proteomics Analysis

The heat shock protein 70 gene as a new alternative molecular marker for the taxonomic identification of Streptomyces strains

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