Rapid assay of the monoamine content in small volumes of rat plasma

Cheng, Fu‐Chou; Yang, Lin‐Lan; Kuo, Jon‐Son; Yang, Meng; Yu, Pi-Chin

doi:10.1016/0378-4347(93)e0410-r

Cited by 13 publications

(6 citation statements)

References 19 publications

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“…This may imply that urine levels of HVA and HIAA can be used not only as a diagnostic index for neuroblastoma, but also as a potential reference for the size of tumor in the brain. The present method combines an HPLC method (Hadfield et al, 1986) with a plasma extraction method (Cheng et al, 1994), resulting in lower sample volumes (50 mL), and less solvent-front problems due to perchloric acid, which is the extraction reagent in the previous method (Hadfield et al, 1986). Other methods for diagnosis of neuroblastoma include ELISA (Shi et al, 1998) and isotope dilution GC/MS (Fauler et al, 1997).…”

Section: Resultsmentioning

confidence: 99%

“…The mobile phase was filtered through a 0.2 mm filter and degassed for 20 min before use. For analysis of the media components, 50 mL of the filtrates prepared from the culture media were mixed with 5 mL (1 ng/mL) of the internal standard isoproterenol (Cheng et al, 1994) and injected for analysis. Stock solutions of the standards (1 mg/mL in 5% perchloric acid, stored at À70°C) were diluted with deionized water to 20 ng/mL as working solutions.…”

Section: Methodsmentioning

confidence: 99%

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Simultaneous HPLC of twelve monoamines and metabolites shows neuroblastoma cell line releases HVA and HIAA

Liu

Cheng

Chen

et al. 2000

Biomed. Chromatogr.

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Neuroblastoma is a solid tumor occurring usually in children less than 5 years old. It has been difficult to distinguish neuroblastoma from other childhood tumors through morphological diagnosis. Urine homovanillic acid (HVA), which is a metabolite of dopamine, has been proposed as a diagnostic index. Although increased levels of a serotonin metabolite, 5-hydroxyindole-3-acetic acid (HIAA), have also been observed in urine samples of the patients, they were largely attributed to dietary amines. By using an HPLC system with electrochemical detection, which can simultaneously assay 12 monoamines and metabolites, we showed that HVA and HIAA are two of the most prominent monoamine metabolites in the medium after a neuroblastoma cell line (IMR-32) was cultured for 3 days. Moreover, we found that the levels of HVA and HIAA in the media are proportional to the cell densities. These results suggest that the levels of HVA and HIAA in tissue culture media, or in urine from patients whose dietary amines are well controlled, may provide a valuable diagnostic index for neuroblastoma.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Simultaneous HPLC of twelve monoamines and metabolites shows neuroblastoma cell line releases HVA and HIAA

Liu

Cheng

Chen

et al. 2000

Biomed. Chromatogr.

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“…17 The chromatographic system consisted of a GL Science model 576 solvent delivery pump (Lake Forest, CA, USA), a Rheodyne 7725 (Rheodyner Cotati, CA, USA) injector and a BAS LC-4C electrochemical detector with a glassy carbon electrode. The columns used were a C 18 column (5 mm, 250 Â 4.6 mm) and a C 18 guard column (5 mm, 30 Â 4.6 mm) from Luna, (Phenomenex, Torrance, CA, USA).…”

Section: Hplc Chromatographymentioning

confidence: 99%

Fluoxetine increases extracellular levels of 3‐methoxy‐4‐hydroxyphenylglycol in cultured COLO320 DM cells

Yue

Liu

2004

Cell Biochemistry & Function

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Fluoxetine (Prozac) is a serotonin reuptake inhibitor. It increases extracellular levels of serotonin and is used in relieving the depressive symptoms of cancer patients. It has been reported that the drug may enhance the growth of certain cancer cells. This study investigates whether fluoxetine enhances the growth of a human colon cancer cell line (COLO320 DM) and if it affects the extracellular levels of serotonin or its metabolite, 5-hydroxyindole-3-acetic acid (5-HIAA) and other monoamines and metabolites at two cell densities. The extracellular levels of serotonin, 5-HIAA and other monoamines and metabolites were measured simultaneously by high performance liquid chromatography from cell-culture media after incubation of cells both with and without fluoxetine for 3 days. The viability of COLO320 DM cells was evaluated using 3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). At low cell densities (1.25x10(5) cells ml-1), fluoxetine at 1-10 microM significantly increased the extracellular levels of serotonin (p<0.005), 5-HIAA (p<0.005), and 3-methoxy-4-hydroxyphenylglycol (MHPG; p<0.001) as compared to the controls. Fluoxetine at 10-100 microM significantly inhibited the growth of COLO320 DM (p<0.005). At high cell densities (2x10(6) cells ml-1), fluoxetine at 1-10 microM significantly increased the extracellular levels of MHPG (p<0.01), and at 10 microM it significantly increased the extracellular levels of 5-HIAA (p<0.05). Fluoxetine at 100 microM significantly inhibited the growth of the cells (p<0.0001). These results suggest that fluoxetine at 1 microM of effective concentration may increase the extracellular levels MHPG, in addition to serotonin and 5-HIAA levels, yet not inhibit the growth of COLO320 DM.

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“…To evaluate the changes in amounts of catecholamines and their metabolites in RAM and culture medium, concentrations of epinephrine, norepinephrine, dopamine, 3-metboxy-4-hydroxymandelic acid (MHPG), and homovanillic acid (HVA) were measured by high performance liquid chromatography (HPLC) reverse phase separation analysis as described below which was a modification of the methods reported by Mfiller and Unsicker (1981) and Cheng et al (1994). RAM and 1 ml of culture medium were collected on Day 0 through Day 7.…”

Section: Concentrations Of Catecholamines In the Medulla And Mediummentioning

confidence: 99%

Characterization of the rat adrenal medulla cultured in vitro

Fujinaga

Chen

Scott

1999

In Vitro Cell.Dev.Biol.-Animal

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A wide variety of experimental animal models have been used to investigate the mechanisms of synthesis, storage, and release of catecholamines. Whereas in vivo experimental models are situated at one end of the spectrum, cell culture models are situated at the other end. In the present study, we have characterized various aspects of the rat adrenal medulla cultured in vitro as a whole tissue, aiming to establish a new experimental model in between in vivo animal models and cell culture models. We adapted a bottle rotator system commonly used for culturing rodent whole embryos. Changes in histology, activities and mRNA levels of catecholamine-synthesizing enzymes, and concentrations of catecholamines in the adrenal medulla were studied. In addition, the effects of cholinergic stimulation on catecholamine release from the adrenal medulla were examined. Overall the results indicate that various aspects of the adrenal medulla become stable after 4 d of culture and the adrenal medulla at this stage releases catecholamines in response to cholinergic stimulation. The whole adrenal medulla culture system may be a useful tool for investigating catecholamine-related functions dependent on intercellular reactions or communications.

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Rapid assay of the monoamine content in small volumes of rat plasma

Cited by 13 publications

References 19 publications

Simultaneous HPLC of twelve monoamines and metabolites shows neuroblastoma cell line releases HVA and HIAA

Simultaneous HPLC of twelve monoamines and metabolites shows neuroblastoma cell line releases HVA and HIAA

Fluoxetine increases extracellular levels of 3‐methoxy‐4‐hydroxyphenylglycol in cultured COLO320 DM cells

Characterization of the rat adrenal medulla cultured in vitro

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