Rapid and ultrasensitive electromechanical detection of ions, biomolecules and SARS-CoV-2 RNA in unamplified samples

Wang, Liqian; Wang, Xuejun; Wu, Yungen; Guo, Mingquan; Gu, Cheng; Dai, Changhao; Kong, Dewen; Wang, Yao; Zhang, Cong; Qu, Di; Fan, Chunhai; Xie, Youhua; Zhu, Zhiming; Liu, Yunqi; Wei, Dacheng

doi:10.1038/s41551-021-00833-7

Cited by 171 publications

(244 citation statements)

References 65 publications

Supporting

Mentioning

241

Contrasting

Unclassified

Order By: Relevance

“…Importantly, our platform also integrates microfluidics to enable automated sample preparation, temperature control, and reagent addition steps, thereby creating an easy-to-use sample-to-answer platform that is more appropriate for POC applications. Novel graphene FET-based EC biosensors for SARS-CoV-2 have displayed similar levels of sensitivity to the 0.8 ct/μl LOD shown here 67 , but this earlier assay was only validated for VTM-based nasopharyngeal swab samples and more significantly, it does not have multiplexing capabilities. With our integrated device, we are able to perform both serological and RNA detection from a saliva sample, which does not require specialized collection reagents or equipment, and the results are reported concurrently.…”

Section: Discussionmentioning

confidence: 71%

Lab-on-a-chip multiplexed electrochemical sensor enables simultaneous detection of SARS-CoV-2 RNA and host antibodies

Puig

Timilsina

Rainbow

et al. 2021

Preprint

View full text Add to dashboard Cite

There continues to be a great need for rapid, accurate, and cost-effective point-of-care devices that can diagnose the presence of SARS-CoV-2 virus and development of IgG and IgM antibody responses in early and late stages of COVID-19 disease. Here, we describe a versatile multiplexed electrochemical (EC) sensor platform modified with an antifouling nanocomposite coating that enables single-molecule CRISPR/Cas-based molecular detection of SARS-CoV-2 viral RNA with on-chip signal validation as well as multiplexed serological detection of antibodies against three SARS-CoV-2 viral antigens. The CRISPR-based EC platform achieved 100% accuracy for detection of viral RNA and showed an excellent correlation with RT-qPCR using 30 clinical saliva samples. The serology EC platform obtained 100% sensitivity and 100% specificity for anti-SARS-CoV-2 IgG, as well as 94% specificity and 82% sensitivity for anti-SARS-CoV-2 IgM with 112 clinical plasma samples. These data demonstrate that integration of CRISPR-based RNA detection and serological assays with antifouling nanocomposite-based EC sensors enables performance as good or better than traditional laboratory-based techniques.

show abstract

Section: Discussionmentioning

confidence: 71%

Lab-on-a-chip multiplexed electrochemical sensor enables simultaneous detection of SARS-CoV-2 RNA and host antibodies

Puig

Timilsina

Rainbow

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Through an electromechanical approach, this design achieved direct ultrasensitive and rapid detection of SARS-CoV-2 RNA in less than four minutes. 135 Considering that DNA nanostructures can be chemically and biologically synthesized on a large scale and the merits of simple fabrication and a mild detection environment, these dynamic DNA nanostructure-based rapid biomedical sensors could serve as potent tools early in future public health emergencies. 136 …”

Section: Prospects and Challenges In The Use Of Dynamic Dna Nanostruc...mentioning

confidence: 99%

Prospects and challenges of dynamic DNA nanostructures in biomedical applications

Tian

Lin

2022

Bone Res

View full text Add to dashboard Cite

The physicochemical nature of DNA allows the assembly of highly predictable structures via several fabrication strategies, which have been applied to make breakthroughs in various fields. Moreover, DNA nanostructures are regarded as materials with excellent editability and biocompatibility for biomedical applications. The ongoing maintenance and release of new DNA structure design tools ease the work and make large and arbitrary DNA structures feasible for different applications. However, the nature of DNA nanostructures endows them with several stimulus-responsive mechanisms capable of responding to biomolecules, such as nucleic acids and proteins, as well as biophysical environmental parameters, such as temperature and pH. Via these mechanisms, stimulus-responsive dynamic DNA nanostructures have been applied in several biomedical settings, including basic research, active drug delivery, biosensor development, and tissue engineering. These applications have shown the versatility of dynamic DNA nanostructures, with unignorable merits that exceed those of their traditional counterparts, such as polymers and metal particles. However, there are stability, yield, exogenous DNA, and ethical considerations regarding their clinical translation. In this review, we first introduce the recent efforts and discoveries in DNA nanotechnology, highlighting the uses of dynamic DNA nanostructures in biomedical applications. Then, several dynamic DNA nanostructures are presented, and their typical biomedical applications, including their use as DNA aptamers, ion concentration/pH-sensitive DNA molecules, DNA nanostructures capable of strand displacement reactions, and protein-based dynamic DNA nanostructures, are discussed. Finally, the challenges regarding the biomedical applications of dynamic DNA nanostructures are discussed.

show abstract

“… [39] , [40] , [41] For instance, some nanomaterials-based biosensors match the accuracy, sensitivity, and specificity for an RNA-extraction-free nano-amplified test of SARS-CoV-2 using color readout. [42] , [43] Compared to the color sensor, some fluorescence detection can be more sensitive without using the amplification or extraction processes. [44] , [45] , [46] This may significantly reduce the waste generated during analysis progresses.…”

Section: Introductionmentioning

confidence: 99%

Prospects of NIR fluorescent nanosensors for green detection of SARS-CoV-2

Li¹,

Zhou²,

Sun³

et al. 2022

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

Rapid and ultrasensitive electromechanical detection of ions, biomolecules and SARS-CoV-2 RNA in unamplified samples

Cited by 171 publications

References 65 publications

Lab-on-a-chip multiplexed electrochemical sensor enables simultaneous detection of SARS-CoV-2 RNA and host antibodies

Lab-on-a-chip multiplexed electrochemical sensor enables simultaneous detection of SARS-CoV-2 RNA and host antibodies

Prospects and challenges of dynamic DNA nanostructures in biomedical applications

Prospects of NIR fluorescent nanosensors for green detection of SARS-CoV-2

Contact Info

Product

Resources

About