Rapid and ultrasensitive detection of 3-amino-2-oxazolidinone in catfish muscle with indirect competitive enzyme-linked immunosorbent and immunochromatographic assays

Abstract

In this study, we developed an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic assay to detect the furazolidone metabolite, 3-amino-2oxazolidinone (AOZ). The detection of AOZ was based on the AOZ derivative 3-([2-nitrophenyl]methyleneamino]-2-oxazolidinone (2-NPAOZ). 3-([(3-Carboxyphenyl)methylene]-amino)-2-oxazolidinone (2-CPAOZ) was used as the immunizing hapten and a conjugate of AOZ and glyoxylic acid (AOZ-A) was used as the coating hapten. The monoclonal anti-2-NPAOZ antibody, 5G12, was generated with a half-inhibitory concentration (IC 50) of 0.2 ng/mL and a line of 0.06-0.66 ng/mL. Its cross reactivity with other analogues was less than 8%. Spiked catfish samples (0.1, 0.2, and 0.5 μg/kg) were analyzed with the proposed system. The ic-ELISA showed a recovery range of 86.2-118.5% and the intra-assay and inter-assay coefficients of variation ranged from 4.3% to 9.4%. Under the optimum assay conditions, the immunochromatographic assay had a visual cutoff value of 0.5 μg/kg in catfish samples. Both methods can be used to detect AOZ in catfish samples.

Development of an immunochromatographic strip test for rapid detection of sodium nifurstyrenate in fish

“…Immunochromatographic assay test strips, also known as lateral flow immunoassay (LFIA) test strips are becoming increasingly popular as diagnostic tools for the detection of target analytes because they are simple to use, they are rapid, specific, and sensitive. The major advantages of this method is that all reagents are included on the strip and results can be obtained within 5-10 min (Ding, Liu, Song, Kuang, & Xu, 2017;Kong et al, 2017;Liu et al, 2017;Liu, Xu, Suryoprabowo, Song, & Kuang, 2018b;Li, Liu, Song, & Kuan, 2018).…”

Rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip

“…Immunochromatographic assay test strips, also known as lateral flow immunoassay (LFIA) test strips, are becoming increasingly popular as diagnostic tools for detecting target analytes because they are simple to use, rapid, specific, and sensitive. The advantages of this method are that all of the reagents are included in the strip and results can be obtained within 5-10 min (Ding, Liu, Song, Kuang, & Xu, 2017;Kong et al, 2017aKong et al, , 2017bWang, Xie, et al, 2017).…”

Development of ic-ELISA and lateral-flow immunochromatographic strip for detection of vitamin B2 in an energy drink and vitamin tablets

“…The reaction was stopped with the addition of 2 M sulfuric acid (50 µL/well). Absorbance was measured at 450 nm (Ding, Liu, Song, Kuang, & Xu, 2017). IC 50 and LOD were calculated by a standard curve generated by plotting optical density at 450 nm (OD 450 ) on the y-axis against vitamin B 2 concentration on the x-axis.…”