Rapid and Straightforward Quantification of Recombinant Proteins Using Fluorescence Polarization

Continuous Processes and Flow Chemistry at the Universities of Applied Sciences in Switzerland

“…This approach was applied to the rapid mixing of proteins with denaturants in a microfluidic circuit to explore protein folding using Fourier-Transform Infrared Spectroscopy (FTIR). [21] Another development tackled the (quasi) real-time monitoring of fermentation processes (PAT ), which is highly demanded in the biopharmaceutical industry. In this respect, a successful collaboration between HES-SO Va lais Wallis and HES-SO Fribourg [10] led to the development of a quick and specific analytical method to follow the production of His-tagged proteins.…”