Rapid and stable production of 2,3-butanediol by an engineered <i>Saccharomyces cerevisiae</i> strain in a continuous airlift bioreactor

Yamada, R.; Nishikawa, Riru; Wakita, Kazuki; Ogino, Hiroyasu

doi:10.1007/s10295-018-2033-5

Cited by 8 publications

(6 citation statements)

References 28 publications

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“…Consequently, non-pathogenic 2,3-butanediol producers are promising candidates for industrial production processes [15, 16]. Genetic engineering enables 2,3-butanediol production with well-characterized strains like Escherichia coli [17–20], Saccharomyces cerevisiae [21, 22], Zymomonas mobilis [23] and Corynebacterium glutamicum [24, 25]. Furthermore, some Bacillus and Paenibacillus strains naturally produce 2,3-butanediol [15, 26].…”

Section: Introductionmentioning

confidence: 99%

Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production

et al. 2019

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Background Production of 2,3-butanediol from renewable resources is a promising measure to decrease the consumption of fossil resources in the chemical industry. One of the most influential parameters on biotechnological 2,3-butanediol production is the oxygen availability during the cultivation. As 2,3-butanediol is produced under microaerobic process conditions, a well-controlled oxygen supply is the key parameter to control biomass formation and 2,3-butanediol production. As biomass is on the one hand not the final product, but on the other hand the essential biocatalyst, the optimal compromise between biomass formation and 2,3-butanediol production has to be defined. Results A shake flask methodology is presented to evaluate the effects of oxygen availability on 2,3-butanediol production with Bacillus licheniformis DSM 8785 by variation of the filling volume. A defined two-stage cultivation strategy was developed to investigate the metabolic response to different defined maximum oxygen transfer capacities at equal initial growth conditions. The respiratory quotient was measured online to determine the point of glucose depletion, as 2,3-butanediol is consumed afterwards. Based on this strategy, comparable results to stirred tank reactors were achieved. The highest space–time yield (1.3 g/L/h) and a 2,3-butanediol concentration of 68 g/L combined with low acetoin concentrations and avoided glycerol formation were achieved at a maximum oxygen transfer capacity of 13 mmol/L/h. The highest overall 2,3-butanediol concentration of 78 g/L was observed at a maximum oxygen transfer capacity of 4 mmol/L/h. Conclusions The presented shake flask approach reduces the experimental effort and costs providing a fast and reliable methodology to investigate the effects of oxygen availability. This can be applied especially on product and by-product formation under microaerobic conditions. Utilization of the maximum oxygen transfer capacity as measure for the oxygen availability allows for an easy adaption to other bioreactor setups and scales. Electronic supplementary material The online version of this article (10.1186/s12934-019-1126-9) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production

et al. 2019

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“…Because operational parameters vary for different environment‐medium‐microorganism, the optimal value should be established individually for each strain and substrate used. More recently, some studies have focused on the genetic modification of yeasts and bacteria toward the production of 2,3‐BD, but the reported results of productivity and yields of this chemical are often low when compared to values obtained using wild‐type strains …”

Section: Introductionmentioning

confidence: 99%

Production of 2,3‐butanediol byKlebsiella pneumoniaeBLh‐1 andPantoea agglomeransBL1 cultivated in acid and enzymatic hydrolysates of soybean hull

Cortivo

Machado

Hickert

et al. 2019

Biotechnology Progress

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We investigated the production of 2,3-butanediol by two enterobacteria isolated from an environmental consortium, Klebsiella pneumoniae BLh-1 and Pantoea agglomerans BL1, in a bioprocess using acid and enzymatic hydrolysates of soybean hull as substrates. Cultivations were carried out in orbital shaker under microaerophilic conditions, at 30 C and 37 C, for both bacteria. Both hydrolysates presented high osmotic pressures, around 2,000 mOsm/kg, with varying concentrations of glucose, xylose, and arabinose. Both bacteria were able to grow in the hydrolysates, at both temperatures, and they efficiently converted sugars into 2,3-butanediol, showing yields varying from 0.25 to 0.51 g/g of sugars and maximum 2,3-butanediol concentrations varying from 6.4 to 21.9 g/L. Other metabolic products were also obtained in lower amounts, notably ethanol, which peaked at 3.6 g/L in cultures using the enzymatic hydrolysate at 30 C. These results suggest the potential use of these recently isolated bacteria to convert lignocellulosic biomass hydrolysates into value-added products. K E Y W O R D S 2,3-butanediol, bioprocess, Klebsiella pneumoniae, lignocellulosic biomass hydrolysates, Pantoea agglomerans, soybean hull

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“…,3-Butanediol (BDO) is a highly attractive building block [1][2][3][4] that can be produced in high titers of up to 120 g L −1 via fermentation of biomass-derived sugars 5 using engineered Zymomonas mobilis owing to its low toxicity to the producing microorganisms. [6][7][8][9][10][11][12] As BDO is a fourcarbon molecule, fewer energy intensive C-C bond forming reactions are needed relative to C1 and C2 building blocks (e.g. methanol and ethanol) when constructing more complex carbon scaffolds for fuel applications.…”

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Separation, recovery and upgrading of 2,3‐butanediol from fermentation broth

Rajale

Yang

Judge

et al. 2023

Biofuels Bioprod Bioref

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2,3-Butanediol (BDO) is a bio-derived building block available from biomass through biochemical methods in high titers (>120 g L −1 ) making it an attractive target for production and further upgrading to chemical products and fuels such as sustainable aviation fuel. A key challenge to enable the adoption of BDO as a precursor is the effective separation and isolation of this molecule from the fermentation broth. 2,3-Butanediol has a boiling point higher than that of water (177°C), and as a consequence, separation via distillation methods is an energy-intensive and therefore costly approach. We have improved the BDO separation through conversion to a 1,3-dioxolane directly in fermentation broth via reaction with bio-derived aldehydes catalyzed by a solid acid catalyst. The resulting dioxolane phase separates from the fermentation broth, allowing for easy decantation and isolation in >90% isolated yield. Isolated dioxolane can be used directly as a compression iginition fuel, trans-acetalized to recover high-purity BDO or used directly in a catalytic process as a BDO synthon to produce methyl ethyl ketone with aldehyde recovery in near quantitative yield.

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Rapid and stable production of 2,3-butanediol by an engineered Saccharomyces cerevisiae strain in a continuous airlift bioreactor

Cited by 8 publications

References 28 publications

Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production

Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production

Production of 2,3‐butanediol byKlebsiella pneumoniaeBLh‐1 andPantoea agglomeransBL1 cultivated in acid and enzymatic hydrolysates of soybean hull

Separation, recovery and upgrading of 2,3‐butanediol from fermentation broth

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