Rapid and Specific Identification of Genus Cynoglossus by Multiplex PCR Assays Using Species-specific Derived from the COI Region

Noh, Eun Soo; Kang, Hyun Jung; An, Cheul Min; Park, Jung Youn; Kim, Eun Mi; Kang, Jung Ha

doi:10.5352/jls.2016.26.9.1007

Cited by 7 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several researchers have studied the sizes of DNA fragments in the PCR profiles of five species of Eastern Pacific abalone (genus Haliotis ) (Muchmore et al, 1998), black tiger shrimp ( Penaeus monodon ) (Tassanakajon et al, 1998), the brittle star ( Amphiura filiformis ) (McCormack et al, 2000) and wild shrimp populations (Yoon & Kim, 2003a). The specific primer proved useful in the identification of individuals and/or populations, resulting from variations in DNA polymorphisms among individuals/populations (Liu et al, 1998; Yoon & Kim, 2003b; Kim et al, 2004; Yoon & Kim, 2004; Kwun & Kim, 2010; Noh et al, 2016). The PCR method, using several primers, was applied to the identification of three endemic Spanish barbel species: Barbus bocagei , B. graellsii and B. sclateri (Callejas & Ochando, 1998).…”

Section: Resultsmentioning

confidence: 99%

“…This confirms that the method is a suitable tool for DNA comparisons, both within and between individuals, species, and populations. As a rule, using various oligonucleotides primers, this PCR method has been applied to identify specific markers specific to species and geographical population, as well as genetic diversity/polymorphism in diverse species of organisms (McCormack et al, 2000; Yoon & Kim, 2003a; Kim et al, 2004; Kwun & Kim, 2010; Noh et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

“…However, in spite of their economic and scientific consequences, a little information currently exists regarding the physiological and ecological levels of tonguesole species in Korea. Nevertheless, this tonguesole, which is distinguished important reproductively (Lee et al., 2000; Kang et al, 2012; Lim et al, 2016), morphologically (Choi, 1996; Gu et al, 2009; Kwun & Kim, 2010), as well as ecologically (Choi et al, 1995; Baeck & Huh, 2004) are a few genetically and/or molecular-biologically (Kwun & Kim, 2010; Noh et al, 2016) studied like other finfishes. There are more wishes to understand the genetic traits and conformation of this fish population in order to evaluate precisely the genetic consequences.…”

Section: Introductionmentioning

confidence: 95%

“…The specific markers particular to species, genus or geographical population have all been employed in the identification and discrimination of individuals, species and populations, hybrid parentages and genetic diagnostics (Callejas & Ochando, 1998; Huang et al, 2000; Kim et al., 2000; Yoon & Kim, 2004). Thus, the applications of PCR techniques to aquaculture had been to identify genetic distances among a few of fish species and/or invertebrates apart from geographic sites (Partis & Wells, 1996; Callejas & Ochando, 1998; McCormack et al, 2000; Kwun & Kim, 2010; Song & Yoon, 2013; Oh & Yoon, 2014; Yoon, 2015; Noh et al, 2016). In the present study, to elucidate the genetic distances and differences between geographical tonguesole populations, the author performed a clustering analysis of two tonguesole populations collected from two sites of Gunsan and the Atlantic.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Genetic Distances Within-Population and Between-Population of Tonguesole, Cynoglossus spp. Identified by PCR Technique

Yoon¹

2019

Dev. Reprod.

View full text Add to dashboard Cite

The higher fragment sizes (>2,100 bp) are not observed in the two C. spp. populations. The six oligonucleotides primers OPA-11, OPB-09, OPB-14, OPB-20, OPC-14, and OPC-18 were used to generate the unique shared loci to each tonguesole population and shared loci by the two tonguesole populations. The hierarchical polar dendrogram indicates two main clusters: Gunsan (GUNSAN 01–GUNSAN 11) and the Atlantic (ATLANTIC 12–ATLANTIC 22) from two geographic populations of tonguesoles. The shortest genetic distance displaying significant molecular difference was between individuals’ GUNSAN no. 02–GUNSAN no. 01 (genetic distance=0.038). In the long run, individual no. 02 of the ATLANTIC tonguesole was most distantly related to GUNSAN no. 06 (genetic distance=0.958). These results demonstrate that the Gunsan tonguesole population is genetically different from the Atlantic tonguesole population. The potential of PCR analysis to identify diagnostic markers for the identification of two tonguesole populations has been demonstrated. As a rule, using various oligonucleotides primers, this PCR method has been applied to identify polymorphic/specific markers particular to species and geographical population, as well as genetic diversity/polymorphism in diverse species of organisms.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic Distances Within-Population and Between-Population of Tonguesole, Cynoglossus spp. Identified by PCR Technique

Yoon¹

2019

Dev. Reprod.

View full text Add to dashboard Cite

show abstract

“…Umumnya kode sandi DNA target untuk hewan tingkat tinggi adalah sekuen dalam mitokondria sitokrom oksidase subunit 1 atau dikenal sebagai CO1 (Madduppa et al, 2017). Sekuen COI inilah yang sering digunakan untuk identifikasi ikan dan dibuktikan telah mampu mengidentifikasi sepuluh spesies dari genus Cynoglossus yang didapat dari Spanyol, Mozambik, Bangladesh dan Korea (Noh et al, 2016). Dari hasil BLAST di situs NCBI diperoleh spesies Cynoglossus arel dengan persentase kesamaan basa 100% dan nilai Query cover 95%, yaitu persentase kedekatan sekuen yang dianalisis dengan sekuen yang dimiliki oleh spesies yang tercantum.…”

Section: Tabelunclassified

IDENTIFIKASI IKAN LIDAH Cynoglossus arel (Bloch & Schneider, 1801) BERDASARKAN MORFOMETRIK DAN DNA BARCODING YANG DIPERDAGANGKAN DI TEMPAT PELELANGAN IKAN MUARA ANGKE

Alina¹,

Madduppa

2020

BAWAL Widya Riset Perikanan Tangkap

View full text Add to dashboard Cite

Ikan lidah merupakan ikan ekonomis penting yang menjadi salah satu komoditas yang diperdagangkan di Tempat Pelelangan Ikan (TPI) Muara Angke. Ikan lidah memiliki keunikan karena metamorfosis mereka, kebiasaan membenamkan diri dalam substrat dan sirip pektoralnya yang terdegradasi sedangkan sirip lainnya saling bertemu. Terdapat enam spesies ikan lidah dari famili Cynoglossidae yang dapat ditemukan di Indonesia, sedangkan penelitian terkait identifikasi ikan lidah masih terbatas. Penelitian ini bertujuan untuk mengidentifikasi dan memastikan spesies ikan lidah yang diperdagangkan di TPI Muara Angke berdasarkan pengukuran morfometrik dan DNA Barcoding menggunakan sekuen COI. Hasil analisis morfologi menggunakan literatur menunjukkan bahwa ikan lidah sampel memiliki kemiripan karakteristik morfologi dengan Cynoglossus lingua dan Cynoglossus arel yang kemudian diyakini sebagai C. arel berdasarkan ciri diagnostiknya. Berdasarkan analisis DNA barcoding diperoleh spesies Cynoglossus cf. arel dengan identifikasi kesamaan 100% yang tercantum dalam GenBank. Dari hasil analisis morfologi dan DNA barcoding menunjukkan bahwa ikan lidah yang diperdagangkan di TPI Muara Angke merupakan ikan dari famili Cynoglossidae, genus Cynoglossus, spesies Cynoglossus arel.Tongue Fish Identification Traded in Muara Angke Fish Auction Based on Morphometric and DNA Barcoding Using COI Sequences. Tongue fish is an important economical fish which is one of the commodities traded at Muara Angke Fish Auction. Tongue fish are unique because of their metamorphosis, their habit of immersing themselves in the substrate, and their pectoral fins degraded while the other three fins are confluent. There are six species of tongue fish from the Cynoglossidae family that can be found in Indonesia, whereas the study about tongue fish identifications are limited. The present study aims to identify and clarify the species of tongue fish traded at Muara Angke Fish Auction based on morphometric measurements and DNA Barcoding using the COI sequence. The results of the morphological analysis using the literature showed that the tongue fish samples had similar morphological characteristics with Cynoglossus lingua and Cynoglossus arel which were then believed to be C. arel based on their diagnostic characteristics. DNA barcoding analysis of tongue fish sample shows Cynoglossus cf. arel with 100% similarity identification listed in GenBank. From the results of morphological analysis and DNA barcoding showed that the tongue fish traded at TPI Muara Angke are fish from the family Cynoglossidae, genus Cynoglossus, species of Cynoglossus arel.

show abstract

Development and validation of multiplex PCR assay for differentiating tunas and billfishes

Kim

Park

Lee

et al. 2021

Food Sci Biotechnol

View full text Add to dashboard Cite

Rapid and Specific Identification of Genus Cynoglossus by Multiplex PCR Assays Using Species-specific Derived from the COI Region

Abstract: A highly efficient, rapid, and reliable multiplex polymerase chain reaction based method for distinguishing ten species of genus Cynoglossus (C.

Cited by 7 publications

References 0 publications

Genetic Distances Within-Population and Between-Population of Tonguesole, Cynoglossus spp. Identified by PCR Technique

Genetic Distances Within-Population and Between-Population of Tonguesole, Cynoglossus spp. Identified by PCR Technique

IDENTIFIKASI IKAN LIDAH Cynoglossus arel (Bloch & Schneider, 1801) BERDASARKAN MORFOMETRIK DAN DNA BARCODING YANG DIPERDAGANGKAN DI TEMPAT PELELANGAN IKAN MUARA ANGKE

Development and validation of multiplex PCR assay for differentiating tunas and billfishes

Contact Info

Product

Resources

About