Rapid and Specific Determination of Serotonin, Its Precursors and Metabolites in Rat Brain Tissue by Liquid Chromatography with Electrochemical Detection

A substance that induces germinal vesicle breakdown (GVBD) of Spisula oocytes in vitro was isolated from Spisula body fluid (SBF ) and ganglion cells by ultrafiltration through PM-10 membrane filter, charcoal extraction, and reversed phase HPLC. Its retention time on HPLC elution is coincident with that of reference serotonin (5-hydroxytryptamine, 5-HT) creatinine sulfate. The HPLC-purified substance from SBF and nerve ganglion induced GVBD in Spisit/a oocytes in a dose-dependent manner. 5-HT was identified in Spisula body fluid and ganglion cells by HPLC-electrochemical detection. The present results show that the factor in Spisula body fluid and ganglion cells that induces GVBD in Spisula oocytes in vitro is 5-HT or a closely related compound.

show abstract

“…7). HPLC-ECD is used widely for the determination of 5-HT, its precursors, and metabolites in tissues (Trouvin el a/., 1987).…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Factor with Oocyte Maturation Inducing Activity inSpisula

Kadam

Koide

1989

The Biological Bulletin

View full text Add to dashboard Cite

show abstract

“…The following four regions of the brain were dissected out immediately on ice-cold aluminum surface: frontal cortex, hippocampus, hypothalamus, and brainstem. Brain tissue concentrations of 5-HT and 5-HIAA were measured by high-performance liquid chromatography with electrochemical detection (HPLC-EC) [18,19] . Chromatography was performed with a Jasco pump (PU1580, Tokyo, Japan) equipped with a Decade (Antec Leyden, Netherlands) electrochemical detector with a glassy carbon electrode versus an Ag / AgCl reference electrode (Eox.…”

Section: Study Of the Brainmentioning

confidence: 99%

Effect of β-Endorphin Imprinting during Late Pregnancy on the Brain Serotonin and Plasma Nocistatin Levels of Adult Male Rats

Tekes¹,

Gyenge²,

Hantos³

et al. 2007

Horm Metab Res

View full text Add to dashboard Cite

Female rats were treated with 10 microg of beta-endorphin on the 19th day of pregnancy. Offspring were studied when five months old. Serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) content in four brain regions were determined by HPLC-EC and the nocistatin levels of blood plasma using RIA methods. In each brain region studied, the 5-HT levels were highly significantly reduced and that of 5-HIAA in three regions was highly significantly increased. When 5HIAA/5HT ratios, as a measure of serotonin turnover, were calculated, imprinted animals showed extremely high values. Plasma nocistatin level was also significantly elevated. The results call attention to the effect of perinatal endorphin imprinting and its long-term consequences (e.g., setting of aggressiveness, pain tolerance).

show abstract

“…Brain tissue concentrations of 5-HT were measured by high-performance liquid chromatography (HPLC) with electrochemical detection [20,21]. Chromatography was performed with a Jasco pump (PU1580, Tokyo, Japan) with a Decade (Antec Leyden, Netherlands) electrochemical detector with a glassy carbon electrode vs. an Ag/AgCl reference electrode (Eox.…”

Section: Study Of the Brainmentioning

confidence: 99%

Endorphin Excess at Weaning Durably Influences Sexual Activity, Uterine Estrogen Receptor’s Binding Capacity and Brain Serotonin Level of Female Rats

et al. 2004

View full text Add to dashboard Cite

Perinatally, the first encounter between the maturing receptor and its target hormone results in hormonal imprinting, which adjusts the binding capacity of the receptor for life. In the presence of an excess of the target hormone or foreign molecules than can be bound by the receptor, faulty imprinting carries life-long consequences. In cytogenic organs, imprinting could also be provoked in other periods of life (late imprinting). Imprinting also durably influences the production of the imprinter and related hormones. In the present study, single beta-endorphin doses was given to three-week old female rats at 3 microg/animal, and the serotonin in five brain regions (frontal cortex, striatum, hippocampus, hypothalamus and brain stem) and uterine estrogen receptor content were determined, thymic glucocorticoid receptor binding capacity was measured, and sexual behavior was tested at five months of age. Brain serotonin levels highly significantly decreased, while sexual activity (Meyerson index and lordosis quotient) increased. At the same time, uterine estrogen receptor affinity decreased. There was no change in receptor binding capacity in the thymus. We will go on to discuss interrelations between the results. The experiments demonstrate that a non-perinatal treatment with a molecule acting at receptor level (late imprinting) can also lastingly influence various indexes in non-cytogenic organs. The results call attention to the possible long-lasting influence of an endorphin surge (caused, for example, by pain) on brain serotonin content and sexual behavior.

show abstract

Rapid and Specific Determination of Serotonin, Its Precursors and Metabolites in Rat Brain Tissue by Liquid Chromatography with Electrochemical Detection

Cited by 12 publications

References 23 publications

Characterization of a Factor with Oocyte Maturation Inducing Activity inSpisula

Characterization of a Factor with Oocyte Maturation Inducing Activity inSpisula

Effect of β-Endorphin Imprinting during Late Pregnancy on the Brain Serotonin and Plasma Nocistatin Levels of Adult Male Rats

Endorphin Excess at Weaning Durably Influences Sexual Activity, Uterine Estrogen Receptor’s Binding Capacity and Brain Serotonin Level of Female Rats

Contact Info

Product

Resources

About