Rapid and specific detection of mango (Mangifera indica) in processed food using an isothermal nucleic acid amplification assay

Sheu, Shyang-Chwen; Tsou, Po-Chuan; Lien, Yi-Yang; Lee, Meng-Shiou

doi:10.1007/s00217-020-03440-z

Cited by 6 publications

(3 citation statements)

References 19 publications

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“…The F2/B2 sequences and their complementary F1/B1 (F1c/B1c) sequences are referred to as FIP and BIP. Typically, four primer sequences are enough to amplify a target nucleic acid [26,27], but, to enhance the specificity and efficacy of the reaction, two additional loop primers (LoopF and LoopB) are incorporated in the LAMP reaction mixture [14]. The loop primers identify the area between the F2/B2 and F1/B1 in the sequence [27].…”

Section: Principle and Salient Features Of The Lamp Methodsmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP): A Rapid, Sensitive, Specific, and Cost-Effective Point-of-Care Test for Coronaviruses in the Context of COVID-19 Pandemic

Augustine

Hasan

Das

et al. 2020

Biology

198

166

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The rampant spread of COVID-19 and the worldwide prevalence of infected cases demand a rapid, simple, and cost-effective Point of Care Test (PoCT) for the accurate diagnosis of this pandemic. The most common molecular tests approved by regulatory bodies across the world for COVID-19 diagnosis are based on Polymerase Chain Reaction (PCR). While PCR-based tests are highly sensitive, specific, and remarkably reliable, they have many limitations ranging from the requirement of sophisticated laboratories, need of skilled personnel, use of complex protocol, long wait times for results, and an overall high cost per test. These limitations have inspired researchers to search for alternative diagnostic methods that are fast, economical, and executable in low-resource laboratory settings. The discovery of Loop-mediated isothermal Amplification (LAMP) has provided a reliable substitute platform for the accurate detection of low copy number nucleic acids in the diagnosis of several viral diseases, including epidemics like Severe Acute Respiratory Syndrome (SARS) and Middle East Respiratory Syndrome (MERS). At present, a cocktail of LAMP assay reagents along with reverse transcriptase enzyme (Reverse Transcription LAMP, RT-LAMP) can be a robust solution for the rapid and cost-effective diagnosis for COVID-19, particularly in developing, and low-income countries. In summary, the development of RT-LAMP based diagnostic tools in a paper/strip format or the integration of this method into a microfluidic platform such as a Lab-on-a-chip may revolutionize the concept of PoCT for COVID-19 diagnosis. This review discusses the principle, technology and past research underpinning the success for using this method for diagnosing MERS and SARS, in addition to ongoing research, and the prominent prospect of RT-LAMP in the context of COVID-19 diagnosis.

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Section: Principle and Salient Features Of The Lamp Methodsmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP): A Rapid, Sensitive, Specific, and Cost-Effective Point-of-Care Test for Coronaviruses in the Context of COVID-19 Pandemic

Augustine

Hasan

Das

et al. 2020

Biology

198

166

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“…Tsai et al [98] developed a rapid lateral flow assay (LFA) that uses immunomagnetic nanoparticles (IMNPs) to detect mango major allergen (Man i 1) in food products. A loop-mediated isothermal amplification (LAMP) assay was developed for detection of mango in food [99] . Finally, some studies have shown that early food allergen intake intervention in infants and young children is an effective preventive measure; however, the specific mechanism of its preventive effect is unknown, but it may be related to the induction of allergen-specific immunoglobulin G (IgG) 4 production and the promotion of immune tolerance [100] .…”

Section: Prevention Of Mango Cross-allergymentioning

confidence: 99%

Recent advances in the study of epitopes, allergens and immunologic cross-reactivity of edible mango

Guo¹,

Yanjun²

2024

Food Science and Human Wellness

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Mango (Mangifera indica L.) is a tropical fruit that is widely consumed as both fresh fruits and processed products around the world. The high incidence of mango allergy, on the other hand, has sparked widespread concern. Therefore, a summary and analysis of the current status and issues in mango allergen research can guide in-depth study on the mechanism of mango allergy and reveal effective desensitization methods. We described the incidence of fruit allergy, as well as the mechanism and clinical symptoms of mango allergy, in this review. We also looked into the structural properties of mango allergens, the effect of processing methods on mango allergens, prediction methods for mango allergen epitopes, and the current state of research on mango cross-reactive allergens and preventive measures. Finally, the research directions and ideas for the future are proposed and discussed.

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“…Conversely to LAMP, PCR was strongly affected by steam autoclaved mango samples. [117] LAMP showed equal sensitivity to PCR (LOD of 1 ng), but can be further improved by using loop primers, which are reported to be sensitivity enhancers. [118] Mao et al have reported using LAMP for pistachio Pis V 1 allergens in food samples.…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

Recent advances and challenges in food-borne allergen detection

Sena‐Torralba

Pallás-Tamarit

Morais

et al. 2020

TrAC Trends in Analytical Chemistry

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Rapid and specific detection of mango (Mangifera indica) in processed food using an isothermal nucleic acid amplification assay

Cited by 6 publications

References 19 publications

Loop-Mediated Isothermal Amplification (LAMP): A Rapid, Sensitive, Specific, and Cost-Effective Point-of-Care Test for Coronaviruses in the Context of COVID-19 Pandemic

Loop-Mediated Isothermal Amplification (LAMP): A Rapid, Sensitive, Specific, and Cost-Effective Point-of-Care Test for Coronaviruses in the Context of COVID-19 Pandemic

Recent advances in the study of epitopes, allergens and immunologic cross-reactivity of edible mango

Recent advances and challenges in food-borne allergen detection

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