Rapid and simultaneous determination of three fluoroquinolones in animal-derived foods using excitation-emission matrix fluorescence coupled with second-order calibration method

Ouyang, Yang-Zi; Fang, Huan; Sun, Xiao-Dong; Chang, Yue-Yue; Ding, Yujie J.; Yu, Ru‐Qin

doi:10.1016/j.saa.2019.117458

Cited by 16 publications

(10 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, during this time, at least 10 samples can be prepared simultaneously (this number corresponds to the number of places on the shaker). The overall analysis time of our methodology is similar to that of the MSPE-HPLC–MS method 28 (72 min) or SDME-CE procedure 5 (61 min), but much shorter than in EEM-ANWE approach 10 (155 min).…”

Section: Resultsmentioning

confidence: 72%

“…Cpx and Ofx are derivatives obtained by the introduction of a fluorine atom at the 6th position of the quinoline ring and at the 7th position of the piperazine ring. These compounds are highly effective in the treatment of several diseases, such as bacterial infections of the gastrointestinal tract and urinary tract 10 . Cpx has 12 approved applications by the US Food and Drug Administration (FDA) in the treatment of humans and animals, but is often used for unapproved applications.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simple, fast and reliable CE method for simultaneous determination of ciprofloxacin and ofloxacin in human urine

Kośka

Purgat

Kubalczyk

2022

Sci Rep

View full text Add to dashboard Cite

A simple, fast, and accurate capillary zone electrophoresis method has been developed for the determination of ciprofloxacin and ofloxacin. This method uses liquid–liquid extraction. Therefore, it is characterized by a very simple procedure of sample preparation but at the same time satisfactory precision and accuracy. The extraction process of the same urine sample was repeated three times. The extraction protocol was performed each time for 15 min with 1 mL of dichloromethane and chloroform mixture in a 3:1 volume ratio. A 0.1 mol/L phosphate-borate buffer (pH 8.40) was selected as the background electrolyte. UV detection was performed at 288 nm. The separation was carried out at a voltage of 16 kV, at a temperature of 25 °C. Experimentally evaluated LOQ values for ciprofloxacin and ofloxacin were 0.2 nmol/mL urine and 0.05 nmol/mL urine, respectively. For both analytes the calibration curves exhibited linearity over the entire tested concentration range of 1–6 nmol/mL urine. The precision of the method did not exceed 15%, and the recovery was in the range of 85–115%. The developed and validated procedure was applied to analyze human urine for the content of ciprofloxacin and ofloxacin.

show abstract

Section: Resultsmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Simple, fast and reliable CE method for simultaneous determination of ciprofloxacin and ofloxacin in human urine

Kośka

Purgat

Kubalczyk

2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Compared with the mature technology of blue LEDs, UVC‐LEDs suffer from weak optical output power (Wang et al., 2022), which makes it difficult to obtain sufficient fluorescence emissions from food samples. However, numerous fluorophores in food products, such as aromatic amino acids and nucleic acids (Lenhardt et al., 2017); anthocyanins (Trivittayasil et al., 2016); capsaicinoids (Monago‐Maraña et al., 2018); other fluorophores, such as melamine (Barreto et al., 2021); and fluoroquinolone antibiotics (Ouyang et al., 2020), need to be induced in the UVC region. Therefore, to improve the diversity of devices, future studies should be conducted to solve the heating problem and promote the popularization of UVC‐LEDs in the field of food fluorescence detection.…”

Section: Conclusion and Prospectsmentioning

confidence: 99%

Toward commercial applications of LED and laser‐induced fluorescence techniques for food identity, quality, and safety monitoring: A review

Peng

et al. 2023

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

The assessment of food safety and quality is a matter of paramount importance, especially considering the challenges posed by climate change. Convenient, eco‐friendly, and non‐destructive techniques have attracted extensive attention in the food industry because they can retain food safety and quality. Fluorescence radiation, the process by which fluorophore emits light upon the absorption of ultraviolet or visible light, offers the advantages of high sensitivity and selectivity. The use of excitation–emission matrix (EEM) has been extensively explored in the food industry, but on‐site detection of EEMs remain a challenge. To address this limitation, laser‐induced fluorescence (LIF) and light emitting diode‐induced fluorescence (LED‐IF) have been implemented in many cases to facilitate the transition of fluorescence measurements from the laboratory to commercial applications. This review provides an overview of the application of commercially available LIF/LED‐IF devices for non‐destructive food measurement and recent studies that focus on the development of LIF/LED‐IF devices for commercial applications. These studies were categorized into two stages: the preliminary exploration stage, which emphasizes the selection of an appropriate excitation wavelength based on the combination of EEM and chemometrics, and the pre‐application stage, where experiments were conducted on scouting with specific excitation wavelength. Although commercially available devices have emerged in many research fields, only a limited number have been reported for use in the food industry. Future studies should focus on enhancing the diversity of test samples and parameters that can be measured by a single device, exploring the application of LIF techniques for detecting low‐concentration substances in food, investigating more quantitative approaches, and developing embedded computing devices.

show abstract

“…The overall time needed to prepare and analyze the meat sample for Cpx and Ofx content equals 55 min (during this time, seven samples can be prepared simultaneously due to the seven places in the vortex). This analysis time is shorter in the magnetic solid-phase extraction HPLC-MS [24] (72 min), single drop microextraction CE [12] (61 min), and excitation-emission matrix with an alternating normalization-weighted error (EEM-ANWE) [25] (155 min) procedure.…”

Section: Calibration and Other Validation Datamentioning

confidence: 99%

Simultaneous Determination of Ciprofloxacin and Ofloxacin in Animal Tissues with the Use of Capillary Electrophoresis with Transient Pseudo-Isotachophoresis

et al. 2021

View full text Add to dashboard Cite

We have developed a precise and accurate method for the determination of ciprofloxacin and ofloxacin in meat tissues. Our method utilizes capillary electrophoresis with a transient pseudo-isotachophoresis mechanism and liquid–liquid extraction during sample preparation. For our experiment, a meat tissue sample was homogenized in pH 7.00 phosphate buffer at a ratio of 1:10 (tissue mass: buffer volume; g/mL). The extraction of each sample was carried out twice for 15 min with 600 µL of a mixture of dichloromethane and acetonitrile at a 2:1 volume ratio. We then conducted the electrophoretic separation at a voltage of 16 kV and a temperature of 25 °C using a background electrolyte of 0.1 mol/L phosphate–borate (pH 8.40). We used the UV detection at 288 nm. The experimentally determined LOQs for ciprofloxacin and ofloxacin were 0.27 ppm (0.8 nmol/g tissue) and 0.11 ppm (0.3 nmol/g tissue), respectively. The calibration curves exhibited linearity over the tested concentration range of 2 to 10 nmol/g tissue for both analytes. The relative standard deviation of the determination did not exceed 15%, and the recovery was in the range of 85–115%. We used the method to analyze various meat tissues for their ciprofloxacin and ofloxacin contents.

show abstract

Rapid and simultaneous determination of three fluoroquinolones in animal-derived foods using excitation-emission matrix fluorescence coupled with second-order calibration method

Cited by 16 publications

References 47 publications

Simple, fast and reliable CE method for simultaneous determination of ciprofloxacin and ofloxacin in human urine

Simple, fast and reliable CE method for simultaneous determination of ciprofloxacin and ofloxacin in human urine

Toward commercial applications of LED and laser‐induced fluorescence techniques for food identity, quality, and safety monitoring: A review

Simultaneous Determination of Ciprofloxacin and Ofloxacin in Animal Tissues with the Use of Capillary Electrophoresis with Transient Pseudo-Isotachophoresis

Contact Info

Product

Resources

About