Rapid and Simple Spectrophotometric Analysis of Labetalol Hydrochloride in Pharmaceutical, Urine and Blood Samples

Abstract

There is an increasing interest in new strategies to determine as well as assessment of drugs in medical and pharmaceutical sciences. Herein we describe a simple, rapid, sensitive, and selective spectrophotometric method for determination of labetalol (LBT) hydrochloride based on the oxidation of the drug with ferric ammonium sulphate that yields a green colored product. The increase in absorbance of colored product is measured at 535 nm. All experimental parameters affecting the development of color are investigated and optimized. Under the optimum conditions, Beer's law is obeyed over the concentration range 10-200 µg/mL with molar absorptivity of 2.13 ×10 3 L mol-1 cm-1. The limit of detection (LOD) and limit of quantitation (LOQ) of the proposed method are 2.71 and 8.22 µg/mL, respectively. The intra-day and inter-day precisions and accuracy of the proposed method are acceptable with low values of standard analytical error. The data are in agreement with values obtained by standard British Pharmacopeia (BP) method. The method is successfully applied to the determination of LBT in both pharmaceutical formulations and biological samples (e.g., human urine and plasma) in vitro.

Electrochemical Quantitative Assessment of Labetalol Hydrochloride in Pure Form and Combined Pharmaceutical Formulations

“…Lab is a well-known doping agent in sports and hence has been banned for Olympic players by the International Olympic Committee. [2][3][4] Literature survey revealed that various analytical techniques were employed to estimate the concentration of Lab in pharmaceutical preparations, either alone or in combination with hydrochlorothiazide, and in biological fluids. These techniques include spectrofluorimetry, [5][6][7][8][9][10] chromatography, [11][12][13][14][15][16][17][18] capillary electrophoresis, 19,20 capillary isotachophoresis, 21 NMR spectroscopy, 22 ion-selective electrode using ion-pair complex, 23 and adsorptive voltammetry, 24 methods which in comparison to proposed electrodes require sample manipulation, are affected by various interferences, inappropriate for colored or turbid solutions, and more expensive as they require sophisticated equipment and software for data processing.…”