Rapid and Simple Method for Determination of Cholesterol in Processed Food

Naeemi, Esmaeel D; Ahmad, Nauman; Al-Sharrah, T.; Behbahani, Montaha

doi:10.1093/jaoac/78.6.1522

Cited by 77 publications

(28 citation statements)

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“…Total cholesterol was extracted from freeze-dried meat of the three muscles after direct saponification with saturated methanolic KOH solution (Naeemi, Ahmad, Al-Sharrah, & Behbahani, 1995).…”

Section: Meat Qualitymentioning

confidence: 99%

Dose response of rumen‐protected conjugated linoleic acid supplementation to fattening bulls and heifers on growth, and carcass and meat quality

Schiavon

Cecchinato

Pegolo

et al. 2019

Animal Physiology Nutrition

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We investigated the influence of rumen‐protected conjugated linoleic acid (rpCLA) on growth performances, and carcass and meat quality traits in beef. Twenty‐four young bulls and 30 heifers obtained from double‐muscled beef sires and dairy cows were fed a low‐protein ration (110 g/kg DM of crude protein) supplemented with 0, 8 or 80 g/d of a commercial rpCLA product. The animals were monthly weighed and scored for body muscularity and fatness. Blood samples were collected after 140 days on feed. Animals were slaughtered when they reached average in vivo fatness scores of around 2.5 (heifers) and 2.0 (bulls) points respectively. At slaughter, carcasses, various organs and parts of the gastrointestinal tract were weighed; the 5th rib was dissected and its tissue and muscle chemical composition was determined. The rpCLA had little influence on growth performance but decreased the blood urea content by 28% (p < 0.01). The rpCLA × sex interactions for daily gain (p < 0.05), conformation scores (p < 0.01), and blood creatinine content (p < 0.05) suggest that males were more responsive to rpCLA than females when fed a low‐protein ration, probably because of the metabolic protein‐sparing effect of CLA. Only slight differences were observed in carcass weight and quality at slaughter. The results indicate that the response of beef cattle to rpCLA is dependent on sex or on their propensity for lean and fat accretion. It is also possible that counteracting feedback mechanisms compensate for the influence of rpCLA administration over the course of growth.

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Section: Meat Qualitymentioning

confidence: 99%

Dose response of rumen‐protected conjugated linoleic acid supplementation to fattening bulls and heifers on growth, and carcass and meat quality

Schiavon

Cecchinato

Pegolo

et al. 2019

Animal Physiology Nutrition

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“…The upper layer of oil was taken out. PS content was analyzed by applying GC method as previously described (Naeemi, Ahmad, Alsharrah, & Behbahani, 1995).…”

Section: Solubility In Oilmentioning

confidence: 99%

In vitro bioaccessibility and physicochemical properties of phytosterol linoleic ester synthesized from soybean sterol and linoleic acid

Yang

Oyeyinka

et al. 2018

LWT

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Phytosterols are bioactive components capable of reducing cholesterol level in serum and reducing risk of arteriosclerosis. In this study, conditions for the synthesis of maximum yield of phytosterol linoleic ester (PLE) was optimized and the physicochemical properties and in vitro bioaccessibility of the PLE was assessed. Under the optimized condition of 1:1.1 mole ratio of phytosterol and linoleoyl chloride at 80 o C for 1.5h, the conversion rate of phytosterol reached 96.1%. Its solubility in oil increased 20 times, up to 33.8%. Also, peroxide value of PLE was much lower than linoleic acid (32.9 and 47.0 mmol/kg), which means better oxidative stability. Bioaccessibility of PLE was affected by time, concentration of bile extract, and dissolved medium. It was 4.93% alone, increased by 2.5times compare to phytosterol; or 53.46% in oil, under the condition of 40mg/mL bile extract for 120min. In conclusion, under the tested condition, phytosterol conversion rate, its solubility in oil and bioaccessibility were improved significantly. The method showed great potential in manufacture high quality and quantity of PLE.

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“…The quantification of cholesterol content was based on the experimental procedure of Naemmi et al (1995) modified by Oehlenschla¨ger (2000). It was analysed in a Hewlett Packard 5890 Gas Chromatograph (Palo Alto, CA, USA) and the separation was carried out with helium as carrier gas in a column HP5 (30 m · 0.5 mm i.d.).…”

Section: Cholesterol Analysismentioning

confidence: 99%

Nutritional quality of African catfish Clarias gariepinus (Burchell 1822): a positive criterion for the future development of the European production of Siluroidei

Rosa¹,

Bandarra

Nunes

2007

Int J of Food Sci Tech

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The aims of this study were to evaluate the nutritional quality (proximate composition, amino and fatty acid profiles, cholesterol and mineral contents) of African catfish (Clarias gariepinus) and determine the effect of different cooking methods (grilling, boiling and frying) on biochemical composition. Frying produced the highest water loss and fat gain (P < 0.05). Frying also affected the fatty acid composition significantly (P < 0.05), increasing oleic (18:1n-9) and linoleic (18:2n-6) acid contents. The major essential amino acids were arginine and lysine, and the limiting was the sulphur amino acid methionine. The changes in the cholesterol and mineral contents (K > P > Na > Mg > Ca > Zn > Fe > Cu > Mn) and nutrientrecommended dietary intakes are discussed and several indices (chemical score, amino acid score, essential amino acid index, biological value, nutritional index, retention values, atherogenic index, thrombogenic index and hypercholesterolaemic potential) are presented. The valorisation of these products may stimulate the development of aquaculture production and consumers' interest in Europe.

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Rapid and Simple Method for Determination of Cholesterol in Processed Food

Cited by 77 publications

References 0 publications

Dose response of rumen‐protected conjugated linoleic acid supplementation to fattening bulls and heifers on growth, and carcass and meat quality

Dose response of rumen‐protected conjugated linoleic acid supplementation to fattening bulls and heifers on growth, and carcass and meat quality

In vitro bioaccessibility and physicochemical properties of phytosterol linoleic ester synthesized from soybean sterol and linoleic acid

Nutritional quality of African catfish Clarias gariepinus (Burchell 1822): a positive criterion for the future development of the European production of Siluroidei

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