Rapid and simple detection of Ebola virus by reverse transcription-loop-mediated isothermal amplification

Kurosaki, Yohei; Takada, Ayato; Ebihara, Hideki; Grolla, Allen; Kamo, Naoki; Feldmann, Heinz; Kawaoka, Yoshihiro; Yasuda, Jiro

doi:10.1016/j.jviromet.2006.11.031

Cited by 91 publications

(62 citation statements)

References 27 publications

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“…Though RT-PCR is more effective in detecting EBOV, the disadvantage of this technique is the need for sophisticated instruments that cannot be afforded by most parts of Africa where the disease is reported the most [47]. For the rapid and simple detection of Ebola virus, an efficient reverse-transcription loopmediated isothermal amplification (LAMP) method with high specificity and rapidity has been reported to have been developed, targeting the trailer region of the viral genome [55]. The developed RT-LAMP assay has been reported to be fast that it could detect the virus within 26 minutes.…”

Section: Antigen/virus Detectionmentioning

confidence: 99%

Ebola from emergence to epidemic: the virus and the disease, global preparedness and perspectives

Dhama

Malik

et al. 2015

J Infect Dev Ctries

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Humans constantly encounter threats from many infectious, zoonotic, and devastating pathogens. Outbreaks of severe acute respiratory syndrome (SARS), bird flu, and swine flu posing pandemic threats have compelled health agencies to follow global preparedness for combating the emerging deadly pathogens. The outbreak in West Africa of highly contagious Ebola viral disease (EVD) that started in Guinea in December 2013, assumed global proportions to become the largest outbreak of EVD and the most prominent international health concern. With fatality rates of nearly 50%-90%, it has claimed, as of 11 April 2015, 10,619 human lives out of a total of 25,626 cases reported worldwide. Ebola virus (EBOV), a member of Filoviridae family, is associated with severe, often lethal, hemorrhagic fever disease in humans and animals. The animal hosts, including non-human primates and reservoir hosts (fruit bats), play a significant role in transmission and maintenance of EBOV in nature. Although no approved vaccine for the prevention of EVD currently exists, disease control can be greatly enhanced by timely laboratory confirmation through blood tests using enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR). Adherence to strict sanitary and hygienic measures, monitoring and surveillance of EBOV, as well as quarantine checks on international trade, transport, and visitors from affected countries are mandatory to prevent and control the spread of EVD. This review describes the salient properties of EBOV and the development of novel diagnostics, vaccines, and control strategies for this emerging disease of high public health concern and international emergency.

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Section: Antigen/virus Detectionmentioning

confidence: 99%

Ebola from emergence to epidemic: the virus and the disease, global preparedness and perspectives

Dhama

Malik

et al. 2015

J Infect Dev Ctries

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“…The use of this method has recently been demonstrated for the diagnosis of several human pathogenic RNA viruses (11,14,18). This technique is based on the principle of strand-displacing DNA synthesis by the Bst DNA polymerase with six distinct primers that recognize a total of eight independent sites.…”

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confidence: 99%

Development and Evaluation of a Simple Assay for Marburg Virus Detection Using a Reverse Transcription-Loop-Mediated Isothermal Amplification Method

et al. 2010

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“…RT-LAMP methods for EBOV detection have been previously reported [7,[24][25][26], but all these methods require extraction of RNA from blood samples. Because the reaction developed in the present study is performed at high temperature, lysis and Figure 1.…”

Section: Discussionmentioning

confidence: 99%

Molecular Diagnostic Field Test for Point-of-Care Detection of Ebola Virus Directly From Blood

et al. 2016

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A molecular diagnostic method for robust detection of Ebola virus (EBOV) at the point of care (POC) directly from blood samples is described. This assay is based on reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) of the glycoprotein gene of EBOV. Complete reaction formulations were lyophilized in 0.2-mL polymerase chain reaction tubes. RT-LAMP reactions were performed on a battery-operated isothermal instrument. Limit of detection of this RT-LAMP assay was 2.8 × 10 2 plaque-forming units (PFU)/test and 1 × 10 3 PFU/test within 40 minutes for EBOV-Kikwit and EBOV-Makona, respectively. This assay was found to be specific for the detection of EBOV, as no nonspecific amplification was detected in blood samples spiked with closely related viruses and other pathogens. These results showed that this diagnostic test can be used at the point of care for rapid and specific detection of EBOV directly from blood with high sensitivity within 40 minutes.

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Rapid and simple detection of Ebola virus by reverse transcription-loop-mediated isothermal amplification

Cited by 91 publications

References 27 publications

Ebola from emergence to epidemic: the virus and the disease, global preparedness and perspectives

Ebola from emergence to epidemic: the virus and the disease, global preparedness and perspectives

Development and Evaluation of a Simple Assay for Marburg Virus Detection Using a Reverse Transcription-Loop-Mediated Isothermal Amplification Method

Molecular Diagnostic Field Test for Point-of-Care Detection of Ebola Virus Directly From Blood

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