Rapid and simple analysis of paraquat in tissue homogenate by ultra-high performance liquid chromatography

Merritt, Thomas J.S.; Douglas, Leisa Antoinette; Rzezniczak, Teresa Z.; Watterson, James H.

doi:10.1039/c0ay00759e

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…The amount of PQ recovered supports the underlying mechanism of poor absorption and localization of the PQ in Drosophila brain similar to mammalian models [43], [44].…”

Section: Discussionsupporting

confidence: 65%

“…A non-significant difference in the amount of PQ recovered from different genotypes of exposed Drosophila suggests a nearly equivalent feeding pattern of all tested strains. The amount of PQ recovered supports the underlying mechanism of poor absorption and localization of the PQ in Drosophila brain similar to mammalian models [43] , [44] .…”

Section: Discussionsupporting

confidence: 65%

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Heat Shock Protein-70 (Hsp-70) Suppresses Paraquat-Induced Neurodegeneration by Inhibiting JNK and Caspase-3 Activation in Drosophila Model of Parkinson's Disease

et al. 2014

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Parkinson's disease (PD) is one of the most common neurodegenerative disorders with limited clinical interventions. A number of epidemiological as well as case-control studies have revealed an association between pesticide exposure, especially of paraquat (PQ) and occurrence of PD. Hsp70, a molecular chaperone by function, has been shown as one of the modulators of neurological disorders. However, paucity of information regarding the protective role of Hsp70 on PQ-induced PD like symptoms led us to hypothesize that modulation of hsp70 expression in the dopaminergic neurons would improve the health of these cells. We took advantage of Drosophila, which is a well-established model for neurological research and also possesses genetic tools for easy manipulation of gene expression with limited ethical concern. Over-expression of hsp70 was found to reduce PQ-induced oxidative stress along with JNK and caspase-3 mediated dopaminergic neuronal cell death in exposed organism. Further, anti-apoptotic effect of hsp70 was shown to confer better homeostasis in the dopaminergic neurons of PQ-exposed organism as evidenced by their improved locomotor performance and survival. The study has merit in the context of human concern since we observed protection of dopaminergic neurons in PQ-exposed organism by over-expressing a human homologue of hsp70, HSPA1L, in these cells. The effect was parallel to that observed with Drosophila hsp70. These findings reflect the potential therapeutic applicability of hsp70 against PQ-induced PD like symptoms in an organism.

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“…The amount of PQ recovered supports the underlying mechanism of poor absorption and localization of the PQ in Drosophila brain similar to mammalian models [43], [44].…”

Section: Discussionsupporting

confidence: 65%

Section: Discussionsupporting

confidence: 65%

Heat Shock Protein-70 (Hsp-70) Suppresses Paraquat-Induced Neurodegeneration by Inhibiting JNK and Caspase-3 Activation in Drosophila Model of Parkinson's Disease

et al. 2014

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“…For the accurate qualitative and quantitative concentration determination of paraquat and diquat, various analytical methods have been developed, including high-performance liquid chromatography–ultraviolet detection (HPLC–UV) [ 15 , 18 , 21 , 30 , 35 , 44 , 45 , 46 , 47 ], gas chromatography–mass spectrometry (GC–MS) [ 26 , 28 , 29 , 48 ], liquid chromatography–mass spectrometry (LC–MS/MS and LC–HRMS) [ 13 , 20 , 25 , 27 , 32 , 34 , 49 , 50 , 51 , 52 , 53 , 54 , 55 ], capillary electrophoresis (CE) [ 56 , 57 , 58 ], surface-enhanced Raman spectroscopy (SERES) [ 59 , 60 , 61 ], and electrochemical methods [ 62 , 63 , 64 , 65 ], which we summarize in Figure 2 .…”

Section: Methodsmentioning

confidence: 99%

Paraquat and Diquat: Recent Updates on Their Pretreatment and Analysis Methods since 2010 in Biological Samples

Guo

Gao

2023

Molecules

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Paraquat (PQ) and diquat (DQ) are quaternary ammonium herbicides which have been used worldwide for controlling the growth of weeds on land and in water. However, PQ and DQ are well known to be toxic. PQ is especially toxic to humans. Moreover, there is no specific antidote for PQ poisoning. The main treatment for PQ poisoning is hemoperfusion to reduce the PQ concentration in blood. Therefore, it is essential to be able to detect PQ and DQ concentrations in biological samples. This critical review summarizes the articles published from 2010 to 2022 and can help researchers to understand the development of the sample treatment and analytical methods for the determination of PQ and DQ in various types of biological samples. The sample preparation includes liquid–liquid extraction, solid-phase extraction based on different novel materials, microextration methods, and other methods. Analytical methods for quantifying PQ and DQ, such as different chromatography and spectroscopy methods, electrochemical methods, and immunological methods, are illustrated and compared. We focus on the latest advances in PQ and DQ treatment and the application of new technologies for these analyses. In our opinion, tandem mass spectrometry is a good choice for the determination of PQ and DQ, due to its high sensitivity, high selectivity, and high accuracy. As far as we are concerned, the best LOD of 4 pg/mL for PQ in serum can be obtained.

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Paraquat administration in Drosophila for use in metabolic studies of oxidative stress

Rzezniczak

Douglas

Watterson

et al. 2011

Analytical Biochemistry

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Rapid and simple analysis of paraquat in tissue homogenate by ultra-high performance liquid chromatography

Cited by 4 publications

References 17 publications

Heat Shock Protein-70 (Hsp-70) Suppresses Paraquat-Induced Neurodegeneration by Inhibiting JNK and Caspase-3 Activation in Drosophila Model of Parkinson's Disease

Heat Shock Protein-70 (Hsp-70) Suppresses Paraquat-Induced Neurodegeneration by Inhibiting JNK and Caspase-3 Activation in Drosophila Model of Parkinson's Disease

Paraquat and Diquat: Recent Updates on Their Pretreatment and Analysis Methods since 2010 in Biological Samples

Paraquat administration in Drosophila for use in metabolic studies of oxidative stress

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