Rapid and sensitive quantitation of major constituents in Danggui Buxue Tang by ultra-fast HPLC–TOF/MS

Qi, Lian‐Wen; Cao, Jun; Li, Ping; Wang, Yuxia

doi:10.1016/j.jpba.2008.10.026

Cited by 28 publications

(13 citation statements)

References 16 publications

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“…As a method for quality control, 1 H-NMR metabolic profiling is a faster method (~10 mins) compared to mass spectrometric (GC-MS or LC-MS) approach (~20 mins) [17, 18]. Also, a 1 H-NMR metabolic profile of a biological sample could at once measure different metabolites in an unbiased manner, without the need to define, beforehand, the chemical markers to measure.…”

Section: Discussionmentioning

confidence: 99%

Quality Control of Danggui Buxue Tang, a Traditional Chinese Medicine Decoction, by ¹H‐NMR Metabolic Profiling

Chan

Zhang

Cheung

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Danggui Buxue Tang (DBT) is one of the simplest traditional Chinese medicine (TCM) decoctions, first described in China in 1247 AD. DBT is composed of 2 herbs, Astragali Radix (AR) and Angelica Sinensis Radix (ASR), boiled together in a 5 : 1 ratio. Clinically, DBT is prescribed to women as a remedy for menopausal symptoms. Here, H-NMR metabolic profiling was conducted for DBT and the water extracts of AR or ASR, to evaluate the potential of this chemical profiling method for quality control of the herbal decoction. Principal component analysis (PCA) showed that DBT could be readily distinguished from the water extracts of its constituent herbs by the metabolic profiles. More interestingly, the metabolic profile of DBT was not a simple sum of that of AR and ASR. Asparagine was found at significantly higher concentration in DBT than that in either AR or ASR extract, contributing mainly to the discrimination of DBT sample. In addition, we employed the same method to profile a commercial DBT powder, verifying its authenticity as compared to our prepared DBT. This study is the first to employ H-NMR metabolic profiling for the quality control of traditional Chinese medicine decoctions.

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Section: Discussionmentioning

confidence: 99%

Quality Control of Danggui Buxue Tang, a Traditional Chinese Medicine Decoction, by ¹H‐NMR Metabolic Profiling

Chan

Zhang

Cheung

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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“…Peaks 1, 4, 11, 14, 15 and 17 were, respectively attributed to morroniside, paeoniforin, physcion, ginsenoside-R 0 , astragaloside A and 20(S)-ginsenoside-Rg 3 , by comparison with the retention times and mass spectral data of the reference standards. Utilizing Agilent MassHunter Qualitative Analysis software and searching the Spectral Database for Organic Compounds (SDBS) 5 , or comparing with the literature data [6][7][8][9][10][11][12][13][14] , fourteen peaks were identified. They were loganin 6 (peak 2), echinacoside 7 (peak 3), cistanoside A 8 (peak 5), verbascoside 9 (peak 6), hesperidin 10 (peak 7), ginsenoside 20 (S)-Rf 2 11 (peak 8), tenuifoliside A 12 (peak 9), 2'-acetyl acteoside 13 (peak 10), pseudoginsenoside F11 11 (peak 12), ginsenoside Rg 2 11 (peak 13), astragaloside II 14 (peak 16), 20(R)-ginsenoside-Rg 3 11 (peak 18), ginsenoside-Rk 1 11 (peak 19) and ginsenoside-Rg 5 11 (peak 20).…”

Section: Hplc-q-tof/ms Analysis Of Lbpmentioning

confidence: 99%

Characterization of the Constituents in Rat Biological Fluids After Oral Administration of Longshen Buyi Paste by High-Performance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry

Li¹,

Cong²,

Yao³

et al. 2014

Asian J. Chem.

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“…Calycosin-7-O-β-D-glucoside and ononin (a 7-O-β-D-glucopyranoside of formononetin) are two major avonoid glucosides in DBT [17], and which are reported to have potential e cacy associated with diabetes [18]. The pharmacological e cacy of avonoid glucosides is usually ascribed to their corresponding aglycones that are absorbed much easier than their glycoside forms [19,20].…”

Section: Introductionmentioning

confidence: 99%

Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

Guo

Gao

et al. 2020

Preprint

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Background: Danggui Buxue Tang (DBT), an ancient Chinese herbal decoction containing Astragali Radix and Angelicae Sinensis Radix at a ratio of 5: 1, is prescribed for menopausal women. Flavonoids and its flavonoid glycosides are considered as the major active ingredients within the herbal decoction; however, their amount is not controllable during the preparation. Besides, the aglycons within DBT are believed to have better gut absorption and pharmacological efficacy. Methods: The herbal extract of DBT was fermented with Lactobacillus plantarum. The amounts of flavonoid glucosides and its aglycones in the fermented product were analyzed by using UPLC-MS/MS. In addition, in vitro assays were employed to evaluate the efficacy of the fermented DBT in regulating the activities of α-glucosidase, α-amylase and lipase, as well as their antioxidant capacity (DPPH and T-OAC assays) and anti-glycation property (BSA-methylglyoxal, BSA-fructose, and arginine-methylglyoxal models).Results: The fermentation of DBT with L. plantarum drove a completed conversion of calycosin-7-O-β-D-glucoside and ononin to calycosin and formononetin, respectively. The chemical transformation could be probably mediated by β-glycosidase within the fermented product. Several in vitro assays corresponding to anti-diabetic functions were compared between parental DBT against its fermented product, which included the activities against α-glucosidase, α-amylase and lipase, as well as anti-oxidation and anti-glycation. The fermented DBT showed increased activities in inhibiting α-glycosidase, suppressing DPPH radical-scavenging and anti-glycation, as compared to the original herbal product. Conclusion: These results suggested that DBT being fermented with the probiotic L. plantarum could pave a new direction for fermentation of herbal extract, as to strengthen its pharmacological properties in providing health benefits.

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Rapid and sensitive quantitation of major constituents in Danggui Buxue Tang by ultra-fast HPLC–TOF/MS

Cited by 28 publications

References 16 publications

Quality Control of Danggui Buxue Tang, a Traditional Chinese Medicine Decoction, by ¹H‐NMR Metabolic Profiling

Quality Control of Danggui Buxue Tang, a Traditional Chinese Medicine Decoction, by ¹H‐NMR Metabolic Profiling

Characterization of the Constituents in Rat Biological Fluids After Oral Administration of Longshen Buyi Paste by High-Performance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry

Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

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Rapid and sensitive quantitation of major constituents in Danggui Buxue Tang by ultra-fast HPLC–TOF/MS

Cited by 28 publications

References 16 publications

Quality Control of Danggui Buxue Tang, a Traditional Chinese Medicine Decoction, by 1H‐NMR Metabolic Profiling

Quality Control of Danggui Buxue Tang, a Traditional Chinese Medicine Decoction, by 1H‐NMR Metabolic Profiling

Characterization of the Constituents in Rat Biological Fluids After Oral Administration of Longshen Buyi Paste by High-Performance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry

Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

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Quality Control of Danggui Buxue Tang, a Traditional Chinese Medicine Decoction, by ¹H‐NMR Metabolic Profiling

Quality Control of Danggui Buxue Tang, a Traditional Chinese Medicine Decoction, by ¹H‐NMR Metabolic Profiling