Rapid and sensitive quantification of paraquat and diquat in human serum by liquid chromatography/time-of-flight mass spectrometry using atmospheric pressure photoionization

Yoshioka, Naoki; Asano, Masaharu; Kuse, Azumi; Matsuoka, Tomofumi; Akiyama, Yumi; Mitsuhashi, Takao; Nagasaki, Yasushi; Ueno, Yoshio

doi:10.1007/s11419-012-0138-5

Cited by 22 publications

(15 citation statements)

References 27 publications

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“…In a study by Wang et al, ESI, APCI, and APPI were compared in the LC‐MS analysis of 17 illicit drugs and their metabolites from oral fluid, and APPI showed the lowest LODs for most of the analytes; APCI and APPI were however observed to be more susceptible to matrix effects than ESI (Wang, Feng, & Chen, ). APPI has also been used for the LC‐MS analysis of paraquat and diquat, which are widespread herbicides and popular suicide agents, in human serum (Yoshioka et al, ). When compared with ESI, APPI showed 7–10 times better sensitivity.…”

Section: Recent Appi Applicationsmentioning

confidence: 99%

Recent developments in atmospheric pressure photoionization‐mass spectrometry

Kauppila

Syage

Benter

2015

Mass Spectrometry Reviews

108

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Recent developments in atmospheric pressure photoionization (APPI), which is one of the three most important ionization techniques in liquid chromatography-mass spectrometry, are reviewed. The emphasis is on the practical aspects of APPI analysis, its combination with different separation techniques, novel instrumental developments - especially in gas chromatography and ambient mass spectrometry - and the applications that have appeared in 2009-2014. © 2015 Wiley Periodicals, Inc. Mass Spec Rev 36:423-449, 2017.

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Section: Recent Appi Applicationsmentioning

confidence: 99%

Recent developments in atmospheric pressure photoionization‐mass spectrometry

Kauppila

Syage

Benter

2015

Mass Spectrometry Reviews

108

View full text Add to dashboard Cite

show abstract

“…Also, the sensitivity of the proposed assay is almost same as those of LC-time of flight-MS [14], LC-electrospray ionization-MS [15] and LC-tandem MS [17]. Although the proposed assay is less sensitive than voltammetry [9] and GC-ion trap-MS [10], these methods required special and complex equipment.…”

Section: Optimization Of Chemiluminescence Conditionsmentioning

confidence: 94%

“…Several methods have been reported for the analysis of paraquat using colorimetry [6], spectrophotometry [7], voltammetry [8], capillary electrophoresis (CE) [9], gas chromatography (GC) [10,11], high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection [12], post-column colorimetric detection [13] and mass spectrometry (MS) [14][15][16][17]. The colorimetry using hydrosulfite as a reagent is very simple and traditional assay for paraquat and has been used for on-site analysis.…”

Section: Introductionmentioning

confidence: 99%

A simple and rapid chemiluminescence assay for on-site analysis of paraquat using a portable luminometer

et al. 2013

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Paraquat (N,N´-dimethyl-4,4´-bipyridinium dichloride) is one of the most widely used herbicides owing to its high efficacy and low environmental persistence. However, since paraquat has significant acute toxicity, fatalities are often caused by accidental or voluntary ingestion of paraquat. In consideration of the strong toxicity and fast-acting property of paraquat, on-site analysis in accident scenes should be effective for the immediate medical treatment.In this study, a simple and rapid chemiluminescence assay for on-site analysis of paraquat was developed using a portable luminometer.The proposed assay is based on luminol chemiluminescence detection of superoxide anion radical resulting from the redox reaction between paraquat and dithiothreitol. An intense chemiluminescence was observed by mixing of paraquat and dithiothreitol in the presence of luminol. Since the chemiluminescence intensity was proportional to the concentration of paraquat, a quantitative measurement of paraquat was possible.The calibration curve for standard paraquat solution was linear from 0.025 to 2.5 µM with the correlation coefficient of 0.992, and the detection limit (blank + 3SD) was 22 nM. The proposed assay could be applied to determine paraquat in beverage samples with a cation exchange clean-up procedure. Since the portable luminometer used in this study is small and lightweight, the proposed assay should be useful to on-site analysis of paraquat.

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“…PQ DQ HPLC-UV 10 11 LC-TOF-MS 12 GC-MS 13 14 HPLC-UV Paraquat and diquat are most widely used herbicides, but they have significant acute toxicity to humans. The aim of the present study is to develop a high-performance liquid chromatography (HPLC)-chemiluminescence (CL) detection system that allows simultaneous determinations of paraquat and diquat.…”

Section: Pq Dq Pq Dqmentioning

confidence: 99%