Rapid and sensitive methods for detection of Allorhizobium vitis, causal agent of grapevine crown gall

Habbadi, Khaoula; Benbrahim, Basma; Benbouazza, Abdellatif; Benkirane, Rachid; Achbani, El Hassan

doi:10.22161/ijeab/2.4.45

Cited by 3 publications

(2 citation statements)

References 18 publications

(26 reference statements)

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“…Of these different secretion systems, the type VI secretion system (T6SS) is present in nearly 25% of Gram-negative bacteria with at least one T6SS encoded in their genome. T6SS is known to perform a wide range of functions in host-associated commensals, pathogens, as well as free-living bacteria ( 5 – 14 ). The T6SS is a needle-like contact-dependent molecular nanomachine that injects toxic effectors directly into cognate eukaryotic and prokaryotic cells ( 15 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome profiling of type VI secretion system core gene tssM mutant of Xanthomonas perforans highlights regulators controlling diverse functions ranging from virulence to metabolism

Ramamoorthy,

Pena,

Ghosh

et al. 2024

Microbiol Spectr

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Type VI secretion system (T6SS) is a versatile, contact-dependent contractile nano-weapon in Gram-negative bacteria that fires proteinaceous effector molecules directly into prokaryotic and eukaryotic cells aiding in manipulation of the host and killing of competitors in complex niches. In plant pathogenic xanthomonads, T6SS has been demonstrated to play these diverse roles in individual pathosystems. However, the molecular network underlying the regulation of T6SS is still elusive in Xanthomonas spp. To bridge this knowledge gap, we conducted an in vitro transcriptome screen using plant apoplast mimicking minimal medium, XVM2 medium, to decipher the effect of tssM deletion, a core gene belonging to T6SS-cluster i3*, on the regulation of gene expression in Xanthomonas perforans strain AL65. Transcriptomic data revealed that a total of 277 and 525 genes were upregulated, while 307 and 392 genes were downregulated in the mutant strain after 8 and 16 hours of growth in XVM2 medium. The transcript abundance of several genes associated with flagellum and pilus biogenesis as well as type III secretion system was downregulated in the mutant strain. Deletion of tssM of cluster-i3* resulted in upregulation of several T6SS genes belonging to cluster-i3*** and genes involved in biofilm and cell wall biogenesis. Similarly, transcription regulators like rpoN , Pho regulon, rpoE , and csrA were identified to be upregulated in the mutant strain. Our results suggest that T6SS modulates the expression of global regulators like csrA , rpoN , and pho regulons, triggering a signaling cascade, and co-ordinates the expression of suite of virulence factors, stress response genes, and metabolic genes. IMPORTANCE T6SS has received attention due to its significance in mediating interorganismal competition through contact-dependent release of effector molecules into prokaryotic and eukaryotic cells. Reverse-genetic studies have indicated the role of T6SS in virulence in a variety of plant pathogenic bacteria, including the one studied here, Xanthomonas . However, it is not clear whether such effect on virulence is merely due to a shift in the microbiome-mediated protection or if T6SS is involved in a complex virulence regulatory network. In this study, we conducted in vitro transcriptome profiling in minimal medium to decipher the signaling pathways regulated by tssM-i3* in X. perforans AL65. We show that TssM-i3* regulates the expression of a suite of genes associated with virulence and metabolism either directly or indirectly by altering the transcription of several regulators. These findings further expand our knowledge on the intricate molecular circuits regulated by T6SS in phytopathogenic bacteria.

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Section: Introductionmentioning

confidence: 99%

Transcriptome profiling of type VI secretion system core gene tssM mutant of Xanthomonas perforans highlights regulators controlling diverse functions ranging from virulence to metabolism

Ramamoorthy,

Pena,

Ghosh

et al. 2024

Microbiol Spectr

View full text Add to dashboard Cite

show abstract

“…Of these different secretion systems, the type VI secretion system (T6SS) is present in nearly 25% of Gram-negative bacteria with at least one T6SS encoded in their genome. T6SS is known to perform a wide range of functions in host-associated commensals, pathogens as well as free-living bacteria (5)(6)(7)(8)(9)(10)(11)(12)(13)(14). The T6SS is a needle-like contact dependent molecular nanomachine that injects toxic effectors directly into cognate eukaryotic and prokaryotic cells (15).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome profiling of type VI secretion system core genetssMmutant ofXanthomonas perforanshighlights regulators controlling diverse functions ranging from virulence to metabolism

Sivakumar

Pena

Ghosh

et al. 2023

Preprint

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Type VI secretion system (T6SS) is a contact dependent contractile nano-weapon in Gram-negative bacteria that fires effector molecules directly into prokaryotic and eukaryotic cells aiding in manipulation of the host and killing of competitors in complex niches. In plant pathogenic xanthomonads, T6SS has been demonstrated to play diverse roles in individual pathosystems. However, the regulatory circuit involved in mediating biological functions carried out by T6SS are still elusive. To bridge this knowledge gap, we conducted anin vitrotranscriptome screen using plant apoplast mimicking minimal medium, XVM2 medium, to decipher the effect oftssMdeletion, a core gene belonging to T6SS-cluster i3*, on the regulation of gene expression inXanthomonas perforansstrain AL65. Transcriptomic data revealed that a total of 277 and 525 genes were upregulated, while 307 and 392 genes were downregulated in the mutant strain post 8 and 16 hours of growth in XVM2 medium. The transcript abundance of several genes associated with flagellum and pilus biogenesis as well as type III secretion system were downregulated in the mutant strain. Deletion oftssMof cluster-i3* resulted in upregulation of several T6SS genes belonging to cluster-i3*** and genes involved in biofilm and cell wall biogenesis. Transcription regulators likerpoN, Pho regulon,rpoEandcsrAwere identified to be upregulated in the mutant strain. Our results suggest that T6SS modulates the expression of global regulators likecsrA,rpoNandphoregulons triggering a signaling cascade and co-ordinates the expression of suite of virulence factors, stress response genes and metabolic genes.

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Antagonistic effect of Leuconostoc mesenteroides on grapevine crown gall and fire blight

Sabri

Habbadi²,

Achbani³

et al. 2022

Journal of Crop Improvement

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Rapid and sensitive methods for detection of Allorhizobium vitis, causal agent of grapevine crown gall

Abstract: Abstract-

Cited by 3 publications

References 18 publications

Transcriptome profiling of type VI secretion system core gene tssM mutant of Xanthomonas perforans highlights regulators controlling diverse functions ranging from virulence to metabolism

Transcriptome profiling of type VI secretion system core gene tssM mutant of Xanthomonas perforans highlights regulators controlling diverse functions ranging from virulence to metabolism

Transcriptome profiling of type VI secretion system core genetssMmutant ofXanthomonas perforanshighlights regulators controlling diverse functions ranging from virulence to metabolism

Antagonistic effect of Leuconostoc mesenteroides on grapevine crown gall and fire blight

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