Rapid and sensitive liquid chromatography–tandem mass spectrometry method for the estimation of nicorandil in human plasma

Abstract

A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the estimation of nicorandil in human plasma. Nicorandil was extracted from human plasma using solid-phase extraction technique. Imipramine was used as the internal standard. A Betasil C18 column provided chromatographic separation of analytes followed by detection with mass spectrometry. The method involves a rapid solid-phase extraction from plasma, simple isocratic chromatography conditions and mass spectrometric detection that enables detection at nanogram levels. The proposed method has been validated for a linear range of 1.0-500.0 ng/mL with a correlation coefficient of > or =0.9993. The intra-run and inter-run precision and accuracy was within 10.0%. The overall recovery for nicorandil was 63.81%. The total run time was just 3.0 min.

Simultaneous quantitation of nicorandil and its denitrated metabolite in plasma by LC‐MS/MS: application for a pharmacokinetic study

“…Previous reported works for quantitating nicorandil in plasma by LC‐MS/MS employed expensive or time‐consuming methods for sample preparation, such as solid‐phase extraction or plasma deproteinization followed by two‐step liquid–liquid extraction . The sample preparation method developed in the present work was rapid, simple and low cost, besides provide high recovery rates for the analytes.…”

“…Some methods have been reported for quantitating nicorandil in plasma, using high performance liquid chromatography (HPLC) coupled to ultraviolet (UV) detection . Due to the low sensitivity and selectivity of UV detection, mass spectrometry (MS) was employed in some studies . However, as far as we know, no analytical method has been reported for the simultaneous quantitation of nicorandil and its major active metabolite, NHN, in plasma, using LC‐ESI‐MS/MS.…”