Rapid and sensitive lateral flow immunoassay method for determining alpha fetoprotein in serum using europium (III) chelate microparticles-based lateral flow test strips

Liang, Rong-Liang; Xu, Xuping; Liu, Tiancai; Zhou, Jianwei; Wang, Xianguo; Ren, Zhi-Qi; Hao, Fen; Wu, Yixuan

doi:10.1016/j.aca.2015.07.053

Cited by 44 publications

(35 citation statements)

References 35 publications

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“…Thus, the sensitivity of LFA is increased. As compared to conventional fluorescent labels and quantum dots, lanthanide chelates possess distinctive and attractive fluorescent characteristics, such as long fluorescence lifetimes, narrow emission spectra, large stokes shifts and outstanding photostabilities, all of which contribute to high assay sensitivity and accuracy [198]. Antibodies on the conjugation pad were bound to lanthanide-labeled nanoparticles.…”

Section: Lanthanidementioning

confidence: 99%

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Lateral flow assays: Principles, designs and labels

Bahadır

Sezgintürk

2016

TrAC Trends in Analytical Chemistry

481

315

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Section: Lanthanidementioning

confidence: 99%

“…Eu(III) chelate microparticles were used as a label ochratoxin A (OTA) [196], prostate specific antigen (PSA) [197], AFP [198], and cardiac troponin I (cTnI) [191] detection in LFA.…”

Section: Lanthanidementioning

confidence: 99%

Lateral flow assays: Principles, designs and labels

Bahadır

Sezgintürk

2016

TrAC Trends in Analytical Chemistry

481

315

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“…First, we selected a desirable nitrocellulose (NC) membrane which may best fit the design requirement through the specification provided by manufacturers and the experience from our laboratory team [25,26,27]. Different membranes have different physical and chemical attributes, which affect its capillary flow properties.…”

Section: Discussionmentioning

confidence: 99%

Rapid and Sensitive Lateral Flow Immunoassay Method for Procalcitonin (PCT) Based on Time-Resolved Immunochromatography

Shao

Wang

Xie

et al. 2017

Sensors

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Procalcitonin (PCT) is a current, frequently-used marker for severe bacterial infection. The aim of this study was to develop a cost-effective detection kit for rapid quantitative and on-site detection of PCT. To develop the new PCT quantitative detecting kit, a double-antibody sandwich immunofluorescent assay was employed based on time-resolved immunofluorescent assay (TRFIA) combined with lateral flow immunoassay (LFIA). The performance of the new developed kit was evaluated in the aspects of linearity, precision, accuracy, and specificity. Two-hundred thirty-four serum samples were enrolled to carry out the comparison test. The new PCT quantitative detecting kit exhibited a higher sensitivity (0.08 ng/mL). The inter-assay coefficient of variation (CV) and the intra-assay CV were 5.4%–7.7% and 5.7%–13.4%, respectively. The recovery rates ranged from 93% to 105%. Furthermore, a high correlation (n = 234, r = 0.977, p < 0.0001) and consistency (Kappa = 0.875) were obtained when compared with the PCT kit from Roche Elecsys BRAHMS. Thus, the new quantitative method for detecting PCT has been successfully established. The results indicated that the newly-developed system based on TRFIA combined with LFIA was suitable for rapid and on-site detection for PCT, which might be a useful platform for other biomarkers in point-of-care tests.

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“…These excellent properties rendered fluorescent NPs playing an important role for developing highly sensitive LFICS. Different kinds of fluorescence LFICS were studied, such as testing chloramphenicol, nitrated ceruloplasmin, ochratoxin, alpha fetoprotein [16–20]. …”

Section: Introductionmentioning

confidence: 99%

Smartphone-Based Dual-Modality Imaging System for Quantitative Detection of Color or Fluorescent Lateral Flow Immunochromatographic Strips

et al. 2017

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Nowadays, lateral flow immunochromatographic assays are increasingly popular as a diagnostic tool for point-of-care (POC) test based on their simplicity, specificity, and sensitivity. Hence, quantitative detection and pluralistic popular application are urgently needed in medical examination. In this study, a smartphone-based dual-modality imaging system was developed for quantitative detection of color or fluorescent lateral flow test strips, which can be operated anywhere at any time. In this system, the white and ultra-violet (UV) light of optical device was designed, which was tunable with different strips, and the Sobel operator algorithm was used in the software, which could enhance the identification ability to recognize the test area from the background boundary information. Moreover, this technology based on extraction of the components from RGB format (red, green, and blue) of color strips or only red format of the fluorescent strips can obviously improve the high-signal intensity and sensitivity. Fifty samples were used to evaluate the accuracy of this system, and the ideal detection limit was calculated separately from detection of human chorionic gonadotropin (HCG) and carcinoembryonic antigen (CEA). The results indicated that smartphone-controlled dual-modality imaging system could provide various POC diagnoses, which becomes a potential technology for developing the next-generation of portable system in the near future.

show abstract

Rapid and sensitive lateral flow immunoassay method for determining alpha fetoprotein in serum using europium (III) chelate microparticles-based lateral flow test strips

Cited by 44 publications

References 35 publications

Lateral flow assays: Principles, designs and labels

Lateral flow assays: Principles, designs and labels

Rapid and Sensitive Lateral Flow Immunoassay Method for Procalcitonin (PCT) Based on Time-Resolved Immunochromatography

Smartphone-Based Dual-Modality Imaging System for Quantitative Detection of Color or Fluorescent Lateral Flow Immunochromatographic Strips

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