Rapid and sensitive electrophoresis of urinary protein clearly reveals the pathophysiological feature of renal diseases

Sakatsume, Minoru; Kubota, Ryo; Ogawa, Asa; Narita, Ichiei; T, Matsuda; Shiba, Kiyoko; Gejyo, Fumitake

doi:10.1111/j.1440-1797.2006.00739.x

Cited by 20 publications

(17 citation statements)

References 15 publications

(21 reference statements)

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“…On the other hand, increased urinary cystatin C has been recognized as a marker of renal tubular dysfunction (16,17). In addition, urinary leakage of proteins other than albumin (nonalbumin protein [NAP]) can also indicate tubular damage rather than glomerular damage (18). …”

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Urinary Cystatin C and Tubular Proteinuria Predict Progression of Diabetic Nephropathy

et al. 2013

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OBJECTIVEThe aim of this study was to evaluate the association of urinary cystatin C, a tubular damage marker, with the progression of type 2 diabetic nephropathy.RESERCH DESIGN AND METHODSThe baseline values of serum and urinary cystatin C were measured as primary parameters and those of urinary nonalbumin protein (NAP) were measured as secondary parameters. In this prospective observational study, a total of 237 type 2 diabetic patients were followed up for 29 months (13–44 months).RESULTSBoth the urinary cystatin C-to-creatinine ratio (CCR) and NAP-to-creatinine ratio (NAPCR) were significantly different according to the degree of albuminuria. Both markers had strongly positive correlations at baseline. After adjusting for several clinical factors, both urinary CCR and NAPCR had significant associations with the decline of the estimated glomerular filtration rate (eGFR) (r = 0.160, P = 0.021; r = 0.412, P < 0.001, respectively). Urinary CCR had positive correlations with the decline of eGFR in the subpopulation of patients with eGFR ≥60 mL/min/1.73 m2. In patients with eGFR ≥60 mL/min/1.73 m2 and normoalbuminuria, only urinary NAPCR showed a significant association with the decline of eGFR; urinary CCR did not. In multivariate regression analysis, the number of patients who progressed to chronic kidney disease stage 3 or greater was higher in those in the upper tertiles of both the urinary levels of cystatin C and NAP than in those in the lower tertiles.CONCLUSIONSThe results of this study suggest that urinary cystatin C and NAP may be predictors of the progression of type 2 diabetic nephropathy.

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Urinary Cystatin C and Tubular Proteinuria Predict Progression of Diabetic Nephropathy

et al. 2013

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“…RBP and/or β 2 m with Alb and Tf appeared in the mixed pattern. Since the protein profiles classified with this technique exhibited good agreement with the histological findings of the subsequent renal biopsy , the urinary protein profile based on CAME is expected to become a useful method to predict the regional site and provide supportive data for histopathological diagnosis of renal diseases.…”

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confidence: 97%

Cellulose Acetate Membrane Electrophoresis Based Urinary Proteomics for the Identification of Characteristic Proteins

Nakayama

Kubota

Sakatsume

et al. 2015

Clinical Laboratory Analysis

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“…In addition, it is not clear whether the decrease in glomeru-with tubular toxicity being implicated in the majority of cases (11). Urinary leakage of proteins other than albu-lar filtration rate (GFR) observed in some ITx cohorts is due to the progression of a pretransplant subclinical dia-min can indicate tubular instead of glomerular damage (14) and this nonalbumin pattern of proteinuria has been betic nephropathy (DN) or due to the effect of chronic immunosuppressive therapy or a combination of both. recently described as a risk factor for graft loss and mortality after kidney transplantation (7).…”

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confidence: 99%

Nonalbumin Proteinuria in Islet Transplant Recipients

et al. 2010

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The aim of this study was to evaluate the importance of nonalbumin-predominant proteinuria on kidney function (KF) after islet transplantation (ITx). Twenty-four-hour proteinuria and albuminuria were available in 27 recipients. KF was assessed by serum creatinine and estimated glomerular filtration rate (eGFR) was calculated by Modification of Diet in Renal Disease formula. Correlations between eGFR and albuminuria (r = -0.422, p < 0.001) were higher than with proteinuria (r = -0.223, p < 0.001; p = 0.006 for comparison between correlations). Nineteen (70%) subjects had proteinuria >or= 300 mg/24 h during the follow-up. Subjects were divided into three groups according to urinary protein excretion patterns: no proteinuria (n = 8), nonalbumin-predominant (n = 8), and albumin-predominant (n = 11) proteinuria. Proteinuria >or= 500 mg/24 h was observed only among patients with albumin-predominant proteinuria (64%; p = 0.002) and these patients had the lowest eGFR means post-ITx (no proteinuria: 84.2 +/- 16.4 vs. nonalbumin: 69.1 +/- 13.8 vs. albumin-predominant proteinuria: 65.5 +/- 16.6 ml/min/1.73 m(2), p = 0.044 for first vs. last group). In conclusion, high frequency of proteinuria was observed after ITx. However, it seems to be milder and have less impact on KF when albumin is not the major source of proteinuria. Prospective evaluation of proteinuria, including tubular function markers, should be performed to elucidate the mechanisms of kidney damage in this population.

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Rapid and sensitive electrophoresis of urinary protein clearly reveals the pathophysiological feature of renal diseases

Abstract: The recognition of damaged portions in kidney through the profiles of proteinuria by this system could be practically effective for understanding the kidney disease at bedside.

Cited by 20 publications

References 15 publications

Urinary Cystatin C and Tubular Proteinuria Predict Progression of Diabetic Nephropathy

Urinary Cystatin C and Tubular Proteinuria Predict Progression of Diabetic Nephropathy

Cellulose Acetate Membrane Electrophoresis Based Urinary Proteomics for the Identification of Characteristic Proteins

Nonalbumin Proteinuria in Islet Transplant Recipients

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