Rapid and sensitive determination of levofloxacin in microsamples of human plasma by high‐performance liquid chromatography and its application in a pharmacokinetic study

Abstract

A rapid, sensitive and simple high-performance liquid chromatographic assay with ultraviolet detection was developed for the quantification of levofloxacin in microsamples (100 μL) of human plasma. The extraction procedure included a protein precipitation technique and a short chromatographic running time (4.5 min). Analyses were carried out on a Symmetry C18 column using a mixture of acetonitrile and 0.01 m potassium dihydrogen aqueous solution (pH 3.4; 14:86 v/v) as mobile phase. The method provided specificity and was linear (r ≥ 0.9992) over the concentration range 0.1-12 µg/mL. The average absolute recovery was 93.59%. The intra- and inter-day coefficients of variation were <6%. Additionally, levofloxacin was stable in all evaluations. The usefulness of this method was demonstrated in a pharmacokinetic study of levofloxacin in healthy adult volunteers. The present method offers two main advantages: (a) the use of microsamples reduces the total volume of blood to be collected from patients; and (b) it provides a good cost-effectiveness ratio. It is concluded that the method is rapid, simple, sensitive, economical and suitable for the determination of levofloxacin in human plasma using a small volume of sample.

Ultra-high performance liquid chromatographic determination of levofloxacin in human plasma and prostate tissue with use of experimental design optimization procedures

“…Such described methods include traditional techniques like high performance liquid chromatography with UV detection [21][22][23][27][28][29][30]32,34,36,41] and high performance liquid chromatography with fluorescence (FL) detection [24,26,31,33,37,40,42] as well as new techniques as high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) [25,38,39], ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) [35]. Not all of these assays are appropriate for rapid pharmaceutical analysis of LVF in clinical and research practice.…”

Analytical methods for the determination of certain antibiotics used in critically ill patients

“…HPLC method with ultraviolet detection was developed for the quantification of Levofloxacin in human plasma utilizing a protein precipitation technique and a short chromatographic running time (4.5 min). The separation was achieved on a Symmetry C18 column using a mobile phase consisted of acetonitrile and 0.01 M potassium dihydrogen aqueous solution (pH 3.4; 14:86 v/v) [55] .…”

Personalised dosing of medicines for children

“…Advances in bioanalytical techniques have opened up the potential to use smaller blood volumes (microsamples) to assess drug and biomarker levels in blood or plasma. It is now possible to assess drug concentrations in either dry or wet micro‐blood samples of 5–20 μl . Novel microsampling methods have shown real promise in preclinical and clinical drug development studies and have the potential to revolutionise blood sampling in the home or healthcare environment.…”