Rapid and sensitive determination of fentanyl in dog plasma by on-line solid-phase extraction integrated with a hydrophilic column coupled to tandem mass spectrometry

Chang, Yu-Hao; Yao, Hsien-Tsung; Chao, Yu‐Sheng; Yeh, Teng‐Kuang

doi:10.1016/j.jchromb.2007.07.014

Cited by 29 publications

(18 citation statements)

References 23 publications

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“…This, of course, may result in poor retention of highly hydrophilic analytes [64], but still provides a mechanism by which complex matrices can be fractionated. Both online [65,66] and offline [67 -79] sample preparation with HILIC-ESI-MS, primarily for the analysis of biological fluids, but also for tissues has been reported. For example, a small gel-filtration column has been employed for prepurification of samples [80,81].…”

Section: Pharmacokineticsmentioning

confidence: 99%

The advantages of ESI‐MS detection in conjunction with HILIC mode separations: Fundamentals and applications

Nguyen

Schug²

2008

J of Separation Science

256

133

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The analysis of highly hydrophilic, ionic, and polar compounds has been performed by HILIC-ESI-MS for the last few years. The use of low aqueous/high polar organic solvent content in HILIC separation mobile phase is almost ideal for ESI-MS detection in many cases, resulting in increased sensitivity. Although the addition of modifiers such as acids or salts is necessary in some circumstances for a good separation, the optimum concentrations used are still highly amenable for ESI-MS analysis, showing few deleterious effects. In this review, the mechanism of HILIC separation and ESI ion generation will be briefly discussed, followed by a summary of method development and applications in several fields of research including pharmaceutical, biomolecular, food, metabolic, and environmental analysis.

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Section: Pharmacokineticsmentioning

confidence: 99%

The advantages of ESI‐MS detection in conjunction with HILIC mode separations: Fundamentals and applications

Nguyen

Schug²

2008

J of Separation Science

256

133

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“…Methods using HPLC-UV, [12][13][14][15] GC-MS, 16) and LC-MS, [17][18][19][20][21][22] have been used to determine the fentanyl concentration in plasma [18][19][20]22) and breast milk. 21) Liquid chromatographytandem mass spectrometry (LC-MS/MS) provides a rapid, sensitive, and specific analysis.…”

mentioning

confidence: 99%

A Simple Liquid Chromatography–Tandem Mass Spectrometry Method for Determination of Plasma Fentanyl Concentration in Rats and Patients with Cancer Pain

Hisada

Katoh

Hitoshi

et al. 2013

Biological & Pharmaceutical Bulletin

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A fentanyl patch is widely used for the treatment of cancer pain. Its few adverse effects include constipation and drowsiness. The absorption volume of transdermally applied fentanyl may differ according to its site of application and variability in patch adhesion. Since fentanyl is predominantly metabolized by the drug-metabolizing enzyme cytochrome P450 (CYP) 3A4 in the liver, its concentration may vary in cases of physiologically reduced CYP3A4 activity in the liver (liver disease and aging) or on co-administration of drugs. The clinical significance of measuring plasma concentration of fentanyl is high, but conventional methods require complicated processes such as solid-phase extraction and liquid-liquid extraction before the sample is injected into an HPLC system. In this study, a simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for determining plasma fentanyl concentrations by deproteinization with acetonitrile. A recovery test was conducted using an absolute calibration curve to confirm the method's linearity and inter-and intra-day reproducibility. The required plasma volume for detection was reduced from 1 mL in the conventional method to 20 µL in the present study, and a good calibration curve was obtained in the concentration range from 0.05 to 5 ng/mL. These findings suggest that the method for sample preparation and quantification developed in this study are appropriate for measuring fentanyl concentration in human plasma in clinical settings.

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“…Moreover, among many of analytical techniques, HPLC-UV is the most abundant technique and the least costly, especially when combined to simple liquid extraction unlike SPE [9].…”

Section: Resultsmentioning

confidence: 99%

“…These methods included different types of extraction procedures such as liquid-liquid extraction (LLE) utilizing hexane to extract target analytes [6,7], solid phase extraction (SPE) [9] and single-drop liquid-liquid-liquid micro-extraction (LLME) [10].…”

Section: Introductionmentioning

confidence: 99%

HPLC-UV method development for fentanyl determination in rat plasma and its application to elucidate pharmacokinetic behavior after i.p. administration to rats

Almousa

Ikeda

Wada

et al. 2011

Journal of Chromatography B

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A simple, rapid and validated high performance liquid chromatography method with UV detection for the quantification of an opioid agonist, fentanyl (FEN), in rat plasma was developed. The assay procedure involved chromatographic separation using a ZIC-HILIC SeQUANT column (250 × 4.6 mm, i.d., 5 µm) and a mobile phase of acetonitrile and acetate buffer (pH 3.4, 20 mM) of ratio (= 65:35, v/v) at a flow rate of 1.2 mL/min and detection wavelength of 201 nm. Plasma sample (100 μL) pretreatment was based on simple deprotienization by acetonitrile spiked with clonidine as an internal standard (I.S.) of 20 ng/mL followed by extraction with tert-butyl methyl ether and centrifugation. The organic layer was evaporated under N 2 gas and reconstituted with 100 μL of acetate buffer (pH 3.4, 20 mM), and 50-μL portions of reconstituted sample were injected onto the column. Sample analysis including sample pretreatment was achieved within 35 min. Calibration curve was linear (r ≥ 0.998) from 5 to 100 ng/mL. Both intra-and inter-day assay precisions that are presented through R.S.D were lower than 12.6% for intra-day and lower than 12.0% for inter-day assessment. Limit of detection was 0.8 ng/mL at S/N of 3. This method was omitting the use of expensive solid phase extraction and time consuming liquid extraction procedures. Moreover, the present method was successfully applied to study pharmacokinetic parameters of FEN after intraperitoneal administration to male Wistar rat. Pharmacokinetic parameters estimated by using moment analysis were; T 1/2 198.3 ± 44.7 min, T max 28.3 ± 2.9 min and AUC 0-180 15.6 ± 2.9 (×10 2 ) ng·min/mL.

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Rapid and sensitive determination of fentanyl in dog plasma by on-line solid-phase extraction integrated with a hydrophilic column coupled to tandem mass spectrometry

Cited by 29 publications

References 23 publications

The advantages of ESI‐MS detection in conjunction with HILIC mode separations: Fundamentals and applications

The advantages of ESI‐MS detection in conjunction with HILIC mode separations: Fundamentals and applications

A Simple Liquid Chromatography–Tandem Mass Spectrometry Method for Determination of Plasma Fentanyl Concentration in Rats and Patients with Cancer Pain

HPLC-UV method development for fentanyl determination in rat plasma and its application to elucidate pharmacokinetic behavior after i.p. administration to rats

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