Rapid and Sensitive Detection of Ebola Rna in an Unamplified Sample Based on Crispr-Cas13a and DNA Roller Machine

Hang, Xiao-Min; Liu, Pengfei; Wang, Hui-Yi; Zhao, Kai-Ren; Wang, Li

doi:10.2139/ssrn.4058791

Cited by 1 publication

(3 citation statements)

References 35 publications

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“…Nowadays, the outbreak caused by viruses -e.g. Zika virus, african swine fever virus (ASFV), Ebola virus and SARS-CoV-2 -is a persistent threat to public health and global security [72][73][74]. The mostly recently viral epidemic outbreak is the COVID-19 caused by pathogenic SARS-CoV-2.…”

Section: Viral Infection Diagnosismentioning

confidence: 99%

“…Therefore, CRISPR/Cas-based nucleic acid diagnostics have frequently reported for SARS-CoV-2, and other reported target viruses include ASFV [59,76], Zika virus [6,73,77], Dengue virus [6], Ebola virus [74] and hepatitis B virus [78].…”

Section: Viral Infection Diagnosismentioning

confidence: 99%

“…By combining with a DNA walker machine for amplifying the output signal, a preamplification-free Cas13a assay allowed to detect Ebola RNAs with a LOD of 291 aM (~175 copies RNA/μL). [74]. Nevertheless, design of efficient DNA walker could complicate the nucleic acid assay.…”

Section: Viral Infection Diagnosismentioning

confidence: 99%

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CRISPR-based nucleic acid diagnostics for pathogens

Yang

Zhang

Teng

et al. 2022

Preprint

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Pathogenic infections remain the primary threat for human health, especially the global COVID-19 pandemic. It is important to develop rapid, sensitive and multiplexed tools for detecting pathogens and their mutations, particularly the tailor-made strategies for point-of-care diagnosis allowing for use in resource-constrained settings. The rapidly evolving CRISPR/Cas systems have provided a powerful toolbox for pathogenic diagnostics via nucleic acid tests. In this review, we first describe the resultant promising class 2 (single, multidomain effector) and recently explored class 1 (multisubunit effector complexes) CRISPR tools. We present the diverse engineering nucleic acid diagnostics based on CRISPR/Cas systems for pathogenic viruses, bacteria and fungi, and highlight the application for detecting viral variants and drug-resistant bacteria enabled by CRISPR-based mutation profiling. Finally, we discuss the challenges such as the development of preamplification-free diagnostic assays and present the emerging CRISPR systems and CRISPR cascade that potentially enable multiplexed and preamplification-free pathogenic diagnostics.

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Section: Viral Infection Diagnosismentioning

confidence: 99%