Rapid and sensitive detection of superoxide dismutase in serum of the cervical cancer by 4-aminothiophenol-functionalized bimetallic Au-Ag nanoboxs array

Abstract

Early, efficient and sensitive detection of serum markers in cervical cancer is very important for the treatment and prognosis to cervical cancer patients. In this paper, a SERS platform based on surface enhanced Raman scattering technology was proposed to quantitatively detect superoxide dismutase in serum of cervical cancer patients. Au-Ag nanoboxs array was made by oil-water interface self-assembly method as the trapping substrate. The single-layer Au-AgNBs array was verified by SERS for possessing excellent uniformity, selectivity and reproducibility. 4-aminothiophenol (4-ATP) was used as Raman signal molecule, it will be oxidized to dithiol azobenzene under the surface catalytic reaction with the condition of PH = 9 and laser irradiation. The quantitative detection of SOD could be achieved by calculating the change of characteristic peak ratio. When the concentration was from 10 U mL−1–160 U mL−1, the concentration of SOD could be accurately and quantitatively detected in human serum. The whole test was completed within 20 min and the limit of quantitation was 10 U mL−1. In addition, serum samples from the cervical cancer, the cervical intraepithelial neoplasia and healthy people were tested by the platform and the results were consistent with those of ELISA. The platform has great potential as a tool for early clinical screening of cervical cancer in the future.

Superoxide Dismutase Detection on Silver Nanostructured Substrates through Surface-Enhanced Spectroscopic Techniques

“…The type of substrate and label used, as well as the detection limit, dynamic range and duration of the SOD assays reported in the literature are summarized in Table 1 One of these reports presents the detection of SOD on an Au-Ag nanoboxes array modified with the Raman label 4-aminothiophenol (4-ATP). In the presence of SOD, the oxidation of 4-ATP to dithiol azobenzene (DMAB) upon irradiation of the surface at 785 nm in basic conditions (pH 9) was inhibited, enabling the detection of SOD at concentrations of 10-160 U/mL in 20 min [9]. Since the conversion of U to ng of SOD is not provided in this report, to facilitate comparison with the method developed, the limit of detection and dynamic range for our method is provided in Table 1.…”

“…Although ELISA is a highly sensitive technique, the fact that is laboratory bound and not appropriate for point-of-care determinations led to the development of spectroscopic methods for SOD detection. These methods rely on either surface-enhanced Raman spectroscopy (SERS) [6][7][8][9][10] or surface-enhanced fluorescence (SEF) [11], employing aptamers [6][7][8] or antibodies [10,11] as biorecognition molecules for the determination of SOD levels.…”

Fabrication of Au–Ag Bimetallic Nanoparticles Using Pulsed Laser Ablation for Medical Applications: A Review

“…Among metal nanoparticles, gold, silver, and their composite materials at the nanoscale have garnered significant interest due to their utility in single electron transistors [11], gas sensors [12], bio detectors [13,14], and catalytic processes [15]. Bimetallic nanostructures, which combine both gold (Au) and silver (Ag), have emerged as superior materials.…”